miR-17-5p reduces inflammation and bone erosions in collagen induced arthritis mice and directly targets the JAK-STAT pathway in rheumatoid arthritis fibroblast-like synoviocytes

miR-17-5p reduces inflammation and bone erosions in collagen induced arthritis mice and directly targets the JAK-STAT pathway in rheumatoid arthritis fibroblast-like synoviocytes

Objective: Our objective was to study miR expression in rheumatoid arthritis (RA) phenotype, along with the effects and mechanism of action of miR-17-5p.


Methods:
An array mir done in synovial tissue biopsy of n = 3 naive erosive and non-erosive RA patients. miR-17 lipoplex delivered intra-articularly in a murine model of collagen-induced arthritis. Clinical, histological and structural effects studied for arthritis. In-depth study of miR-17 mechanism-of-action is carried out in SD RA-FLS isolated from synovial tissue.


Results: 55 miRNAs including miR-17 reduced in erosive RA. miR-17 transfection at the foot of clinical rheumatoid reduces inflammation from day 2 to 7 (score 2.8 vs 1.9, p = 0.03). synovial B cells, T cells, macrophages and polynuclear neutrophils infiltrate was significantly reduced. structural damage also decreased as shown by the decrease in the number of osteoclasts (TRAP surface / total surface of 32 vs 18%, p = 0.005) and a score of erosion with the analysis of CT (score 2.9 vs. 1.7, p = 0.023). pro-inflammatory cytokines IL-6 family, and the expression of IL-1β was also reduced significantly, but not TNF. miR17 directly targeting the 3′-translated region of STAT3 and JAK1. STAT3 and JAK1 mRNA and protein expression was reduced in RA-FLS follows miR-17 transfection. STAT3 and JAK1 mRNA and activation of STAT3 assessed by immunohistochemistry was also reduced in the foot injected (% area stained 93 vs 62, p = 0.035).


Conclusions: We demonstrate the role of anti-inflammatory and anti-erosive of miR-17 in vivo. This effect involves the suppression of IL-6 autocrine loop strengthen families through direct targeting JAK1 and STAT3.

 miR-17-5p reduces inflammation and bone erosions in collagen induced arthritis mice and directly targets the JAK-STAT pathway in rheumatoid arthritis fibroblast-like synoviocytes
miR-17-5p reduces inflammation and bone erosions in collagen induced arthritis mice and directly targets the JAK-STAT pathway in rheumatoid arthritis fibroblast-like synoviocytes

The emerging role of fibroblast-like synoviocytes-mediated synovitis in osteoarthritis: Updates

Osteoarthritis (OA), a degenerative disease of the most ubiquitous affecting all the joints, characterized by cartilage degradation and synovial inflammation. Although the pathogenesis of OA is still poorly understood, synovial inflammation is known to play an important role in the development of OA. However, studies in OA pathophysiology have focused more on the degeneration of cartilage and osteophytes and not in the synovium is inflamed and thickened.

Fibroblast like synoviocytes (FLS) produces a series of pro-inflammatory regulator, such as inflammatory cytokines, nitric oxide (NO) and prostaglandin E2 (PGE2). The regulator is positively associated with clinical symptoms of OA, such as inflammatory pain, joint swelling and disease progression. A better understanding of the inflammatory immune response in OA-FLS could provide a new approach for a comprehensive strategy for the treatment of OA.

Here, we have summarized the literature recently published referring to the epigenetic modification, activated signaling pathways and related inflammatory factors involved in inflammation-mediated OA-FLS. In addition, clinical trials related to current and future perspectives are also summarized.

Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Cryopreserved

PBCD4-25-C2M - Ask for price

Positively Selected Normal Peripheral Blood CD19+ B Cells, Cryopreserved

PBCD19P-C10M - Ask for price

Human CD3+ T Cells (Pan T Cells), Cryopreserved

M1299-10 each Ask for price

Human CD3+ T Cells (Pan T Cells), Cryopreserved

M1299-20 each Ask for price

Human CD3+ T Cells (Pan T Cells), Cryopreserved

M1299-30 each Ask for price

Human CD3+ T Cells (Pan T Cells), Cryopreserved

M1299-40 each Ask for price

Human Bone Marrow CD34+ Stem Cells, Cryopreserved

BMCD34-C0.5M - Ask for price

Human Bone Marrow CD34+ Stem Cells, Cryopreserved

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Human Bone Marrow CD34+ Stem Cells, Cryopreserved

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Human Peripheral Blood CD3+ T Cells, Cryopreserved

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Human Cord Blood CD34+ Cells, Cryopreserved, single donor

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Human  Peripheral  Blood  CD4+  T  Lymphocytes, Cryopreserved

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Human  Peripheral  Blood  CD14+ Monocytes, Cryopreserved / Untouched

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Human  Peripheral  Blood  CD14+ Monocytes, Cryopreserved / Positive selection

PBCD14P-C10M - Ask for price

Endothelial Cryopreserve Media

FM300 10 ml
EUR 308.4

CRYOPRESERVATION BAG, 50ML

91-200-88 1/pk
EUR 97.2
Description: Flexible Containers; Bioprocess Bags and Parts

CRYOPRESERVATION BAG, 250ML

91-200-89 1/pk
EUR 100.8
Description: Flexible Containers; Bioprocess Bags and Parts

CRYOPRESERVATION BAG, 500ML

91-200-90 1/pk
EUR 104.4
Description: Flexible Containers; Bioprocess Bags and Parts

CRYOPRESERVATION BAG, 750ML

91-200-91 1/pk
EUR 108
Description: Flexible Containers; Bioprocess Bags and Parts

Cryopreservation Medium For Human Embryonic Stem Cells

HUXES-07031 20/50mL Ask for price

AddexBio Cryopreservation Medium

C0002-20mL RT vial
EUR 124.8

Cryopreservation Medium For General Use

CYRO-10001 20/50mL Ask for price

NCR Cryopreservation Medium For General Use

NCRC-10001 20/50mL Ask for price

Cryopreservation Medium For Neural Stem Cells

GUXNX-07031 20/50mL Ask for price

NCR Protein-Free Cryopreservation Medium For General Use

NCPF-10001 20/50mL Ask for price

Chemical Defined Xeno-free Cell Cryopreservation Medium

abx298009-20ml 20 ml
EUR 292.8

NCR Protein-Free Cryopreservation Medium For Neural Stem Cells

GUXNX-07021 20/50mL Ask for price

COOLCELL SV2 STEMCELL CRYOPRESERVATION SYSTEM INCLUDES THE XT STARTER BENCHTOP COOLER, COOLRACK SV2 AND COOLCELL SV2 CELL FREEZING CONTAINER FOR 12 INJECTABLE VIALS

432012 1/pk
EUR 1024.8
Description: BioCision; BioCision CoolCell

Human Bone Joint PrimaCell: Normal Synovial Cell

2-96011 1 Kit Ask for price

COOLCELL SV10 STEMCELL CRYOPRESERVATION SYSTEM INCLUDES THE XT STARTER BENCHTOP COOLER, COOLRACK SV10 AND COOLCELL SV10 CELL FREEZING CONTAINER FOR 6 INJECTABLE VIALS

432013 1/pk
EUR 1024.8
Description: BioCision; BioCision CoolCell

Human Bone Joint PrimaCell: Normal Synovial Cells Growth Medium

9-46011 5 x 100 ml Ask for price

Human Bone Joint Tissue Preparation Buffer: Normal Synovial Cells

9-80011 1 x 100 ml Ask for price

Rat Bone Joint PrimaCell: Normal Synovial Cells

2-82509 1 Kit Ask for price

Mouse Bone Joint PrimaCell: Normal Synovial Cells

2-82008 1 Kit Ask for price

Rat Bone Joint PrimaCell: Normal Synovial Cells Growth Medium

9-25009 5 x 100 ml Ask for price

Rat Bone Joint Tissue Preparation Buffer: Normal Synovial Cell

9-80216 1 x 100 ml Ask for price

Mouse Bone Joint PrimaCell: Normal Synovial Cells Growth Medium

9-32008 5 x 100 ml Ask for price

Mouse Bone Joint Tissue Preparation Buffer: Normal Synovial Cells

9-80122 1 x 100 ml Ask for price

Human Bone Joint PrimaCell: Normal Synovial Cells Growth Supplements with Serum (for 500 ml medium)

9-47011 1 Set Ask for price

Rat Bone Joint PrimaCell: Normal Synovial Cells Growth Supplements with Serum (for 500 ml medium)

9-26009 1 Set Ask for price

Mouse Bone Joint PrimaCell: Normal Synovial Cells Growth Supplements with Serum (for 500 ml medium)

9-33008 1 Set Ask for price

Total RNA - Human Tumor Tissue: Synoviosarcoma

R1235257-10 10 ug
EUR 400

Normal Human Serum

90R-1002 10 ml
EUR 304.8
Description: Normal Human Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Total Protein - Human Tumor Tissue: Synoviosarcoma

P1235257 1 mg
EUR 471

Normal human exosome RNA

P240 - Ask for price

Human E3 ubiquitin- protein ligase synoviolin, SYVN1 ELISA KIT

ELI-29339h 96 Tests
EUR 988.8

Normal Human Serum (filtered)

90-1013 100 ml
EUR 657.6
Description: Human Serum Off The Clot from Normal Donors

Human E3 ubiquitin-protein ligase synoviolin (SYVN1) ELISA Kit

abx383609-96tests 96 tests
EUR 1093.2

ELISA kit for Human E3 ubiquitin-protein ligase synoviolin (SYVN1)

KTE60446-48T 48T
EUR 398.4
Description: Quantitative sandwich ELISA for measuring Human E3 ubiquitin-protein ligase synoviolin (SYVN1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human E3 ubiquitin-protein ligase synoviolin (SYVN1)

KTE60446-5platesof96wells 5 plates of 96 wells
EUR 2538
Description: Quantitative sandwich ELISA for measuring Human E3 ubiquitin-protein ligase synoviolin (SYVN1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human E3 ubiquitin-protein ligase synoviolin (SYVN1)

KTE60446-96T 96T
EUR 646.8
Description: Quantitative sandwich ELISA for measuring Human E3 ubiquitin-protein ligase synoviolin (SYVN1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Human Normal Tissue Blot II

1522 One blot
EUR 544.2
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are brain, heart, kidney, liver, lung, pancreas, skeletal muscle, skin, and spleen.

Human Normal Tissue Blot VI

1526 One blot
EUR 544.2
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The human normal tissues included in the blot are left cerebellum, right cerebellum, frontal lobe, occipital lobe, parietal lobe, whole eye, spinal cord, temporal lobe, and thalamus.

Universal RNA - Human Normal Tissues

R4234565 4x100 ug
EUR 766

Universal RNA - Human Normal Tissues

R4234565-1 2x100 ug
EUR 450

Synovial Apoptosis Inhibitor 1, Synoviolin Antibody

20-abx115960
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Normal human respiratory tract larynx

RS321 each
EUR 306
Description: Normal human respiratory tract larynx, trachea, bronchi and lung tissue array, 32 cases/32 cores, 2.0 mm core size.

Normal human tissues, 19 organs (2mm)

MNO381 1
EUR 228
Description: Normal tissues from 19 anatomic sites duplicates from 38 individuals, most frequently used for tissue profiling.

cDNA - Human Adult Normal Tissue: Lung

C1234152 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Skin

C1234218 40 reactions
EUR 385

Human Bone PrimaCell: Normal Osteoblasts

2-96012 1 Kit Ask for price

cDNA - Human Adult Normal Tissue: Brain

C1234035 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Colon

C1234090 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Heart

C1234122 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Liver

C1234149 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Ovary

C1234183 40 reactions
EUR 1049

cDNA - Human Adult Normal Tissue: Theca

C1234261-10 10 reactions
EUR 215

Human Fat PrimaCell2: Normal Preadipocyte

2-96063 1 Kit Ask for price

Human Skin PrimaCell2: Normal Melanocytes

2-96102 1 Kit Ask for price

cDNA - Human Adult Normal Tissue: Breast

C1234086 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Kidney

C1234142 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Rectum

C1234206 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Spleen

C1234246 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Testis

C1234260 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Throat

C1234263-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Thymus

C1234264 40 reactions
EUR 1049

cDNA - Human Adult Normal Tissue: Tongue

C1234267-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Tonsil

C1234268-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Uterus

C1234274 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Adipose

C1234003-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Adrenal

C1234004 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Bladder

C1234010 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Trachea

C1234160-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Stomach

C1234248 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Thyroid

C1234265 40 reactions
EUR 385

Human Lung Slide (Normal) (5 slides/pk)

HTS-10101 1 pk
EUR 343.2

cDNA - Human Adult Normal Tissue: Pancreas

C1234188 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Placenta

C1234200 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Prostate

C1234201 40 reactions
EUR 385

Human Liver Slide (Normal) (5 slides/pk)

HTS-10201 1 pk
EUR 343.2

Human Brain Slide (Normal) (5 slides/pk)

HTS-10301 1 pk
EUR 343.2

Human Heart Slide (Normal) (5 slides/pk)

HTS-10501 1 pk
EUR 343.2

cDNA - Human Adult Normal Tissue: Esophagus

C1234106 40 reactions
EUR 385

Normal human tissues, FDA, 33 organs (1.5mm)

MNO341 1
EUR 154.8
Description: 33 types of normal tissues based on the FDA recommendation for antibody cross-reactivity testing.

Human Breast Slide (Normal) (5 slides/pk)

HTS-10001 1 pk
EUR 343.2

Human Kidney Slide (Normal) (5 slides/pk)

HTS-10401 1 pk
EUR 343.2

Human Cervix Slide (Normal) (5 slides/pk)

HTS-11301 1 pk
EUR 343.2

Human Fat PrimaCell1: Normal Adipose Cells

2-96062 1 Kit Ask for price

Human Bladder Slide (Normal) (5 slides/pk)

HTS-12601 1 pk
EUR 343.2

Human Brain PrimaCell10: Normal Neuron Cell

2-96022 1 Kit Ask for price

Normal kidney and normal adjacent kidney tissue array

KDN241a each
EUR 138
Description: Normal kidney and normal adjacent kidney tissue array, 24 cases/ 24 cores

cDNA - Human Adult Normal Tissue: Whole Eye

C1234108-10 10 reactions
EUR 468

Total RNA - Human Adult Normal Tissue: Lung

R1234152-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Skin

R1234218-50 50 ug
EUR 284

Human Testis PrimaCell: Normal Sertoli Cells

2-96105 1 Kit Ask for price

cDNA - Human Adult Normal Tissue: Brain: Pons

C1234071-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Eye: Retina

C1234110-10 10 reactions
EUR 468

cDNA - Human Adult Normal Tissue: Lymph Node

C1234161-10 10 reactions
EUR 215

Total RNA - Human Adult Normal Tissue: Brain

R1234035-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Cecum

R1234089-10 10 ug
EUR 183

Total RNA - Human Adult Normal Tissue: Colon

R1234090-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Heart

R1234122-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Liver

R1234149-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Ovary

R1234183-10 10 ug
EUR 643

Total RNA - Human Adult Normal Tissue: Penis

R1234194-50 50 ug
EUR 170

Nori® Human IL-12 ELISA Kit- Synovial Fluid

GR106141-SF 96-well
EUR 461

Human Skin PrimaCell: Normal Skin Fibroblasts

2-96103 1 Kit Ask for price

Human Brain PrimaCell7: Normal Meningeal Cells

2-96019 1 Kit Ask for price

Human Brain PrimaCell9: Normal Nerve Microglia

2-96021 1 Kit Ask for price

Human Heart PrimaCell2: Normal Atrial Myocytes

2-96067 1 Kit Ask for price

Human Lung PrimaCell1: Normal Lung Fibroblasts

2-96081 1 Kit Ask for price

Universal Protein Lysate: Human Normal Tissues

P4234565 4x0.5 mg
EUR 405

Universal Protein Lysate: Human Normal Tissues

P4234565-1 2x0.5 mg
EUR 270

cDNA - Human Adult Normal Tissue: Bone Marrow

C1234024-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Spinal Cord

C1234234 40 reactions
EUR 385

Human Adult Eye whole (Normal) tissue lysate

HAL-1385 100 ug
EUR 285.6

Human Colorectal Slide (Normal) (5 slides/pk)

HTS-10701 1 pk
EUR 343.2

Total RNA - Human Adult Normal Tissue: Breast

R1234086-50 50 ug
EUR 284

Total RNA - Human Adult Normal Tissue: Kidney

R1234142-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Rectum

R1234206-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Spleen

R1234246-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Testis

R1234260-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Throat

R1234263-10 10 ug
EUR 191

Total RNA - Human Adult Normal Tissue: Thymus

R1234264-10 10 µg
EUR 643

Total RNA - Human Adult Normal Tissue: Tongue

R1234267-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Tonsil

R1234268-10 10 ug
EUR 191

Total RNA - Human Adult Normal Tissue: Uterus

R1234274-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Vagina

R1234283-10 10 ug
EUR 191

Human Brain PrimaCell8: Normal Nerve Astrocytes

2-96020 1 Kit Ask for price

Human Kidney PrimaCell6: Normal Renal Podocytes

2-96079 1 Kit Ask for price

Human Adult Lung (Normal) Whole tissue lysate

HAL-1302 1 mg
EUR 628.8

Human Adult Skin (Normal) Whole tissue lysate

HAL-1376 1 mg
EUR 628.8

Human WI-38 (Lung Fibroblast, normal) lysate

HCL-1226 100ug
EUR 285.6

Total RNA - Human Adult Normal Tissue: Adipose

R1234003-10 10 ug
EUR 183

Total RNA - Human Adult Normal Tissue: Adrenal

R1234004-50 50 ug
EUR 199

Total RNA - Human Adult Normal Tissue: Bladder

R1234010-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Trachea

R1234160-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Parotid

R1234190-50 50 ug
EUR 199

Total RNA - Human Adult Normal Tissue: Stomach

R1234248-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Thyroid

R1234265-50 50 ug
EUR 170

Total RNA - Human Normal Lymphocyte Cell Line

R1254148-1 100 ug
EUR 235

Total RNA - Human Normal Lymphocyte Cell Line

R1254148-2 4 x 100 ug
EUR 787

Human Brain PrimaCell1: Normal Brain Fibroblasts

2-96013 1 Kit Ask for price

Human Eye PrimaCell3: Normal Corneal Fibroblasts

2-96059 1 Kit Ask for price

Human Heart PrimaCell3: Normal Heart Fibroblasts

2-96068 1 Kit Ask for price

Human Lymph PrimaCell1: Normal Endothelial Cells

2-96087 1 Kit Ask for price

cDNA - Human Adult Normal Tissue: Colon Sigmoid

C1234095 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Uterus: Cervix

C1234275 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Uterus: Corpus

C1234276-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Uterus: Fundus

C1234278-10 10 reactions
EUR 215

Total Protein - Human Adult Normal Tissue: Lung

P1234152 1 mg
EUR 188

Total Protein - Human Adult Normal Tissue: Skin

P1234218 1 mg
EUR 188

Human Adult Heart (Normal) Whole tissue lysate

HAL-1301 1 mg
EUR 628.8

Human Adult Brain (Normal) Whole tissue lysate

HAL-1303 1 mg
EUR 628.8

Human Adult Liver (Normal) Whole tissue lysate

HAL-1304 1 mg
EUR 628.8

Human Adult Ovary (Normal) Whole tissue lysate

HAL-1316 1 mg
EUR 628.8

Human Adult Colon (Normal) Whole tissue lysate

HAL-1320 1 mg
EUR 628.8

Total RNA - Human Adult Normal Tissue: Appendix

R1234006-10 10 ug
EUR 191

Total RNA - Human Adult Normal Tissue: Pancreas

R1234188-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Placenta

R1234200-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Prostate

R1234201-50 50 ug
EUR 170

Human Embryo PrimaCell4: Normal Trophoblast Cells

2-96053 1 Kit Ask for price

cDNA - Human Adult Normal Tissue: Brain: Amygdala

C1234036-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Fallopian Tube

C1234115-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Salivary Gland

C1234212-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Stomach: Cardia

C1234250-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Stomach: Corpus

C1234251-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Stomach: Fundus

C1234252-10 10 reactions
EUR 215

Total Protein - Human Adult Normal Tissue: Brain

P1234035 1 mg
EUR 188

Total Protein - Human Adult Normal Tissue: Cecum

P1234089 1 mg
EUR 188

Total Protein - Human Adult Normal Tissue: Colon

P1234090 1 mg
EUR 188

Total Protein - Human Adult Normal Tissue: Heart

P1234122 1 mg
EUR 188

Total Protein - Human Adult Normal Tissue: Liver

P1234149 1 mg
EUR 188

Total Protein - Human Adult Normal Tissue: Ovary

P1234183 1 mg
EUR 771

Total Protein - Human Adult Normal Tissue: Penis

P1234194 1 mg
EUR 188


Collecting evidence suggests that miR-483-3p involved in maintaining the biological properties of human cancers. However, the biological role in rheumatoid arthritis (RA) remains unknown. levels of miR-483-3p in synovial tissue samples and fibroblast-like synoviocytes (FLSs) is determined by using quantitative real-time PCR. The CCK-8 assay and Edu staining was performed to assess cell proliferation in RA FLSs after transfection with miR-483-3p mimics or inhibitors. Flow cytometry with Annexin V-FITC staining or PI staining was performed to assess apoptosis or cell cycle progress in FLSs RA, respectively.

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