miR-17-5p reduces inflammation and bone erosions in collagen induced arthritis mice and directly targets the JAK-STAT pathway in rheumatoid arthritis fibroblast-like synoviocytes

miR-17-5p reduces inflammation and bone erosions in collagen induced arthritis mice and directly targets the JAK-STAT pathway in rheumatoid arthritis fibroblast-like synoviocytes

Objective: Our objective was to study miR expression in rheumatoid arthritis (RA) phenotype, along with the effects and mechanism of action of miR-17-5p.


Methods:
An array mir done in synovial tissue biopsy of n = 3 naive erosive and non-erosive RA patients. miR-17 lipoplex delivered intra-articularly in a murine model of collagen-induced arthritis. Clinical, histological and structural effects studied for arthritis. In-depth study of miR-17 mechanism-of-action is carried out in SD RA-FLS isolated from synovial tissue.


Results: 55 miRNAs including miR-17 reduced in erosive RA. miR-17 transfection at the foot of clinical rheumatoid reduces inflammation from day 2 to 7 (score 2.8 vs 1.9, p = 0.03). synovial B cells, T cells, macrophages and polynuclear neutrophils infiltrate was significantly reduced. structural damage also decreased as shown by the decrease in the number of osteoclasts (TRAP surface / total surface of 32 vs 18%, p = 0.005) and a score of erosion with the analysis of CT (score 2.9 vs. 1.7, p = 0.023). pro-inflammatory cytokines IL-6 family, and the expression of IL-1β was also reduced significantly, but not TNF. miR17 directly targeting the 3′-translated region of STAT3 and JAK1. STAT3 and JAK1 mRNA and protein expression was reduced in RA-FLS follows miR-17 transfection. STAT3 and JAK1 mRNA and activation of STAT3 assessed by immunohistochemistry was also reduced in the foot injected (% area stained 93 vs 62, p = 0.035).


Conclusions: We demonstrate the role of anti-inflammatory and anti-erosive of miR-17 in vivo. This effect involves the suppression of IL-6 autocrine loop strengthen families through direct targeting JAK1 and STAT3.

 miR-17-5p reduces inflammation and bone erosions in collagen induced arthritis mice and directly targets the JAK-STAT pathway in rheumatoid arthritis fibroblast-like synoviocytes
miR-17-5p reduces inflammation and bone erosions in collagen induced arthritis mice and directly targets the JAK-STAT pathway in rheumatoid arthritis fibroblast-like synoviocytes

The emerging role of fibroblast-like synoviocytes-mediated synovitis in osteoarthritis: Updates

Osteoarthritis (OA), a degenerative disease of the most ubiquitous affecting all the joints, characterized by cartilage degradation and synovial inflammation. Although the pathogenesis of OA is still poorly understood, synovial inflammation is known to play an important role in the development of OA. However, studies in OA pathophysiology have focused more on the degeneration of cartilage and osteophytes and not in the synovium is inflamed and thickened.

Fibroblast like synoviocytes (FLS) produces a series of pro-inflammatory regulator, such as inflammatory cytokines, nitric oxide (NO) and prostaglandin E2 (PGE2). The regulator is positively associated with clinical symptoms of OA, such as inflammatory pain, joint swelling and disease progression. A better understanding of the inflammatory immune response in OA-FLS could provide a new approach for a comprehensive strategy for the treatment of OA.

Here, we have summarized the literature recently published referring to the epigenetic modification, activated signaling pathways and related inflammatory factors involved in inflammation-mediated OA-FLS. In addition, clinical trials related to current and future perspectives are also summarized.

Normal Human Serum

90R-1002 10 ml
EUR 304.8
Description: Normal Human Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Human Serum (filtered)

90-1013 100 ml
EUR 657.6
Description: Human Serum Off The Clot from Normal Donors

Human Bone PrimaCell: Normal Osteoblasts

2-96012 1 Kit Ask for price

Human Fat PrimaCell2: Normal Preadipocyte

2-96063 1 Kit Ask for price

Human Skin PrimaCell2: Normal Melanocytes

2-96102 1 Kit Ask for price

Human Normal Tissue Blot II

1522 One blot
EUR 544.2
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are brain, heart, kidney, liver, lung, pancreas, skeletal muscle, skin, and spleen.

Human Normal Tissue Blot VI

1526 One blot
EUR 544.2
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The human normal tissues included in the blot are left cerebellum, right cerebellum, frontal lobe, occipital lobe, parietal lobe, whole eye, spinal cord, temporal lobe, and thalamus.

Multi-normal human tissues array

FDA999w3 each
EUR 266
Description: Multi-normal human tissues array, representing FDA guideline for antibody cross-reactivity testing, 32 types of human organs, 99 cores, each type taken from 3 different individuals (core size 1.5mm).

Multi-normal human tissue array

FDA999y3 each
EUR 355
Description: Multi-normal human tissue array,representing FDA guideline for antibody cross-reactivity testing, 99 cases/99 cores (core size 1.5mm).

Normal human respiratory tract larynx

RS321 each
EUR 306
Description: Normal human respiratory tract larynx, trachea, bronchi and lung tissue array, 32 cases/32 cores, 2.0 mm core size.

Normal kidney and normal adjacent kidney tissue array

KDN241a each
EUR 138
Description: Normal kidney and normal adjacent kidney tissue array, 24 cases/ 24 cores

Normal Goat Serum

9067 50 ml
EUR 120.9
Description: Normal Goat Serum

Normal Bovine Serum

abx098263-100ml 100 ml
EUR 309.6

Normal Goat Serum

88-B9999G000-S0 10 ml
EUR 159.6
Description: Control Normal Goat serum

Normal Canine Serum

88-NC25 100 ml
EUR 727.2
Description: Normal Canine Serum

Normal Horse Serum

88-NE21 100 ml
EUR 36
Description: Control Normal Horse Serum

Normal Goat Serum

88-NG22 500 ml
EUR 36
Description: Control Normal Goat Serum

Normal Goat Serum

88-NG22S 500 ml
EUR 229.2
Description: Control Normal Goat Serum

Normal Mouse Serum

88-NM35 50 ml
EUR 619.2
Description: Normal Mouse Serum

Normal Pig Serum

88-NP 500 ml
EUR 291.6
Description: Normal Pig Serum collected from pigs in the USA

Normal Rabbit Serum

88-NR50 100 ml
EUR 180
Description: Normal Rabbit Serum

Normal Rat Serum

88-NR51 50 ml
EUR 718.8
Description: Normal Rat Serum

Normal Sheep Serum

88-NS55 100 ml
EUR 151.2
Description: Normal Sheep Serum

Normal Mouse Serum

88R-1002 5 ml
EUR 243.6
Description: Normal Mouse Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Rabbit Serum

88R-1004 10 ml
EUR 247.2
Description: Normal Rabbit Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Goat Serum

88R-1006 10 ml
EUR 218.4
Description: Normal Goat Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Sheep Serum

88R-1008 10 ml
EUR 218.4
Description: Normal Sheep Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Rat Serum

88R-1010 5 ml
EUR 242.4
Description: Normal Rat Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Chicken Serum

88R-1014 5 ml
EUR 211.2
Description: Normal Chicken Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Horse Serum

88R-1020 10 ml
EUR 218.4
Description: Normal Horse Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Donkey Serum

88R-1022 10 ml
EUR 218.4
Description: Normal Donkey Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Pig Serum

88R-1023 2 ml
EUR 139.2
Description: Normal Pig (Swine) Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Cat Serum

88R-1026 10 ml
EUR 300
Description: Normal Cat Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Dog Serum

88R-1028 10 ml
EUR 301.2
Description: Normal Dog Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Bovine Serum

88R-B001 100 ml
EUR 198
Description: Control Normal Bovine Serum

Normal Donkey Serum

88R-D001 100 ml
EUR 214.8
Description: Control Normal Donkey Serum

Normal Donkey Serum

88R-D003 10 ml
EUR 178.8
Description: Control Normal Donkey Serum

Normal Goat Serum

88R-NG001 50 ml
EUR 140.4
Description: Normal Goat Serum for use as a control or blocking reagent

Normal Pig Serum

88R-P004 1 liter
EUR 807.6
Description: Control Normal Pig Serum

Normal Sheep Serum

88R-S002 100 ml
EUR 198
Description: Control Normal Sheep Serum

Normal Rabbit Serum

89R-RM001 20 mg
EUR 470.4
Description: Normal Rabbit Serum (Mouse cell adsorbed)

Normal Rabbit Serum

89R-RM002 5 ml
EUR 289.2
Description: Normal Rabbit Serum (Mouse cell adsorbed)

Normal Rabbit Serum

89R-RR001 20 mg
EUR 418.8
Description: Normal Rabbit Serum (Rat cell adsorbed)

Normal Rabbit Serum

89R-RR002 5 ml
EUR 289.2
Description: Normal Rabbit Serum (Rat cell adsorbed)

Normal prostate tissue

PR1002 each
EUR 563
Description: Normal prostate tissue, hyperplasia, cancer adjacent and adjacent normal prostate tissue array, 100 cases/100 cores

Normal male gonad

TEN1201 each
EUR 354
Description: Normal male gonad, seminal fluid pipeline, accessory gland tissue array, 60cases/ 120 cores

Human Bladder PrimaCell2: Normal Bladder Fibroblasts

2-96008 1 Kit Ask for price

Human Brain PrimaCell1: Normal Brain Fibroblasts

2-96013 1 Kit Ask for price

Human Brain PrimaCell7: Normal Meningeal Cells

2-96019 1 Kit Ask for price

Human Brain PrimaCell8: Normal Nerve Astrocytes

2-96020 1 Kit Ask for price

Human Brain PrimaCell9: Normal Nerve Microglia

2-96021 1 Kit Ask for price

Human Brain PrimaCell10: Normal Neuron Cell

2-96022 1 Kit Ask for price

Human Cartilage PrimaCell: Normal Articular Cartilage

2-96043 1 Kit Ask for price

Human Cervix PrimaCell2: Normal Cervical Fibroblasts

2-96045 1 Kit Ask for price

Human Embryo PrimaCell4: Normal Trophoblast Cells

2-96053 1 Kit Ask for price

Human Eye PrimaCell3: Normal Corneal Fibroblasts

2-96059 1 Kit Ask for price

Human Fat PrimaCell1: Normal Adipose Cells

2-96062 1 Kit Ask for price

Human Heart PrimaCell2: Normal Atrial Myocytes

2-96067 1 Kit Ask for price

Human Heart PrimaCell3: Normal Heart Fibroblasts

2-96068 1 Kit Ask for price

Human Heart PrimaCell5: Normal Ventricular Myocytes

2-96070 1 Kit Ask for price

Human Kidney PrimaCell4: Normal Renal Firbroblasts

2-96077 1 Kit Ask for price

Human Kidney PrimaCell6: Normal Renal Podocytes

2-96079 1 Kit Ask for price

Human Lung PrimaCell1: Normal Lung Fibroblasts

2-96081 1 Kit Ask for price

Human Lymph PrimaCell1: Normal Endothelial Cells

2-96087 1 Kit Ask for price

Human Lymph PrimaCell3: Normal Lymphatic Fibroblasts

2-96089 1 Kit Ask for price

Human Mouth PrimaCell: Normal Periodontal Fibroblasts

2-96090 1 Kit Ask for price

Human Ovary PrimaCell2: Normal Ovarian Firbroblasts

2-96095 1 Kit Ask for price

Human Skin PrimaCell1: Normal Epidermal Keratinocytes

2-96101 1 Kit Ask for price

Human Skin PrimaCell: Normal Skin Fibroblasts

2-96103 1 Kit Ask for price

Human Testis PrimaCell: Normal Sertoli Cells

2-96105 1 Kit Ask for price

Universal Protein Lysate: Human Normal Tissues

P4234565 4x0.5 mg
EUR 483.6
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Universal Protein Lysate: Human Normal Tissues

P4234565-1 2x0.5 mg
EUR 342
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Normal human tissues, 19 organs (2mm)

MNO381 1
EUR 228
Description: Normal tissues from 19 anatomic sites duplicates from 38 individuals, most frequently used for tissue profiling.

Universal RNA from Human Normal Tissues

R4234565 4x100 ug
EUR 753.6
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Universal RNA from Human Normal Tissues

R4234565-1 2x100 ug
EUR 468
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Prostate cancer with adjacent normal tissue and normal tissue array

PR1921c each
EUR 594
Description: Prostate cancer with adjacent normal tissue and normal tissue array, including pathology grade, Gleason grade, Gleason score, TNM and clinical stage, PSA Marker result, 96 cases/192 cores, (core size 1.0 mm), replacing PR1921b

Stomach cancer with adjacent normal tissue and normal tissue array

ST2082a each
EUR 474
Description: Stomach cancer with adjacent normal tissue and normal tissue array, including pathology grade, TNM and clinical stage, 96 cases/192 cores, replacing ST2082

Buffered Normal Goat Serum

9066 50 ml
EUR 197.46
Description: Buffered Normal Goat Serum

Testis Tissue Slide (Normal)

11-701-10um 10 um
EUR 241.8

Testis Tissue Slide (Normal)

11-701-4um 4 um
EUR 216.6

Cervix Tissue Slide (Normal)

11-301-10um 10 um
EUR 241.8

Cervix Tissue Slide (Normal)

11-301-4um 4 um
EUR 216.6

Uterus Tissue Slide (Normal)

11-401-10um 10 um
EUR 241.8

Uterus Tissue Slide (Normal)

11-401-4um 4 um
EUR 216.6

Kidney Tissue Lysate (Normal)

1706-02 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-03 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-04 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-05 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Breast Tissue Lysate (Normal)

1710-01 0.1 mg
EUR 260.7
Description: Breast tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Breast Tissue Lysate (Normal)

1710-03 0.1 mg
EUR 260.7
Description: Breast tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Breast Tissue Lysate (Normal)

1710-04 0.1 mg
EUR 260.7
Description: Breast tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Breast Tissue Lysate (Normal)

1710-05 0.1 mg
EUR 260.7
Description: Breast tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Breast Tissue Lysate (Normal)

1710-06 0.1 mg
EUR 260.7
Description: Breast tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Breast Tissue Lysate (Normal)

1710-07 0.1 mg
EUR 260.7
Description: Breast tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Breast Tissue Lysate (Normal)

1710-08 0.1 mg
EUR 260.7
Description: Breast tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Breast Tissue Lysate (Normal)

1710-10 0.1 mg
EUR 260.7
Description: Breast tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Breast Tissue Lysate (Normal)

1710-11 0.1 mg
EUR 260.7
Description: Breast tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Breast Tissue Lysate (Normal)

1710-12 0.1 mg
EUR 260.7
Description: Breast tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-01 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-02 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-03 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-04 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-05 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-06 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-07 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-08 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-10 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-11 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-12 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-13 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-14 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-15 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-16 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-17 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-19 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-20 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-21 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Colon Tissue Lysate (Normal)

1715-22 0.1 mg
EUR 260.7
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Stomach Tissue Lysate (Normal)

1717-01 0.1 mg
EUR 260.7
Description: Stomach tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Liver Tissue Lysate (Normal)

1718-01 0.1 mg
EUR 260.7
Description: Liver tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Liver Tissue Lysate (Normal)

1718-02 0.1 mg
EUR 260.7
Description: Liver tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Bladder Tissue Lysate (Normal)

1721-01 0.1 mg
EUR 260.7
Description: Bladder tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Brain Tissue Lysate (Normal)

1731-01 0.1 mg
EUR 260.7
Description: Brain tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Brain Tissue Lysate (Normal)

1731-02 0.1 mg
EUR 260.7
Description: Brain tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Brain Tissue Lysate (Normal)

1731-03 0.1 mg
EUR 260.7
Description: Brain tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Pancreas Tissue Lysate (Normal)

1736-02 0.1 mg
EUR 260.7
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Pancreas Tissue Lysate (Normal)

1736-05 0.1 mg
EUR 260.7
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Cervix Tissue Lysate (Normal)

1746-01 0.1 mg
EUR 260.7
Description: Cervix tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Cervix Tissue Lysate (Normal)

1746-02 0.1 mg
EUR 260.7
Description: Cervix tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Cervix Tissue Lysate (Normal)

1746-03 0.1 mg
EUR 260.7
Description: Cervix tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Pancreas Tissue Slide (Normal)

11-101-10um 10 um
EUR 241.8

Pancreas Tissue Slide (Normal)

11-101-4um 4 um
EUR 216.6

Ovary Tissue Slide (Normal)

11-201-10um 10 um
EUR 241.8

Ovary Tissue Slide (Normal)

11-201-4um 4 um
EUR 216.6

Lung Tissue Lysate (Normal)

1701-01 0.1 mg
EUR 260.7
Description: Lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Lung Tissue Lysate (Normal)

1701-02 0.1 mg
EUR 260.7
Description: Lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Lung Tissue Lysate (Normal)

1701-03 0.1 mg
EUR 260.7
Description: Lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Lung Tissue Lysate (Normal)

1701-04 0.1 mg
EUR 260.7
Description: Lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Lung Tissue Lysate (Normal)

1701-05 0.1 mg
EUR 260.7
Description: Lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Breast Tissue Slide (Normal)

10-001-10um 10 um
EUR 241.8

Breast Tissue Slide (Normal)

10-001-4um 4 um
EUR 216.6

Lung Tissue Slide (Normal)

10-101-10um 10 um
EUR 241.8

Lung Tissue Slide (Normal)

10-101-4um 4 um
EUR 216.6

Liver Tissue Slide (Normal)

10-201-10um 10 um
EUR 241.8

Liver Tissue Slide (Normal)

10-201-4um 4 um
EUR 216.6

Brain Tissue Slide (Normal)

10-301-10um 10 um
EUR 241.8

Brain Tissue Slide (Normal)

10-301-4um 4 um
EUR 216.6

Kidney Tissue Slide (Normal)

10-401-10um 10 um
EUR 241.8

Kidney Tissue Slide (Normal)

10-401-4um 4 um
EUR 216.6

Heart Tissue Slide (Normal)

10-501-10um 10 um
EUR 241.8

Heart Tissue Slide (Normal)

10-501-4um 4 um
EUR 216.6

Colorectal Tissue Slide (Normal)

10-701-10um 10 um
EUR 241.8

Colorectal Tissue Slide (Normal)

10-701-4um 4 um
EUR 216.6

Stomach Tissue Slide (Normal)

10-801-10um 10 um
EUR 241.8

Stomach Tissue Slide (Normal)

10-801-4um 4 um
EUR 216.6

Spleen Tissue Slide (Normal)

10-901-10um 10 um
EUR 241.8

Spleen Tissue Slide (Normal)

10-901-4um 4 um
EUR 216.6

Gallbladder Tissue Slide (Normal)

12-401-10um 10 um
EUR 241.8

Gallbladder Tissue Slide (Normal)

12-401-4um 4 um
EUR 216.6

Bladder Tissue Slide (Normal)

12-601-10um 10 um
EUR 241.8

Bladder Tissue Slide (Normal)

12-601-4um 4 um
EUR 216.6

Skin Tissue Slide (Normal)

12-701-10um 10 um
EUR 241.8

Skin Tissue Slide (Normal)

12-701-4um 4 um
EUR 216.6

Normal Feline Plasma (EDTA)

abx098254-50ml 50 ml
EUR 844.8

Normal Feline Plasma (Heparin)

abx098255-50ml 50 ml
EUR 878.4

Normal Guinea Pig Serum

88-NP25 50 ml
EUR 264
Description: Normal Guinea Pig Serum

Normal Guinea Pig Serum

88R-1016 5 ml
EUR 170.4
Description: Normal Guinea Pig Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Syrian Hamster Serum

88R-1018 5 ml
EUR 207.6
Description: Normal Syrian Hamster Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Donkey Serum filtered

88R-D1052 100 ml
EUR 267.6
Description: Control Normal Donkey Serum filtered

Normal goat serum(blocking)

AR1009 100mL
EUR 157.2

Spleen Tissue Slide (Normal)

RF10002-03-10um 10 um
EUR 241.8

Spleen Tissue Slide (Normal)

RF10002-03-4um 4 um
EUR 216.6

Trout Liver (Normal) Lysate

THUL-50 50 ug
EUR 255.6

Saline Normal 10 Ml

297753 PK100
EUR 123.12

Glisseal Grease Normal 60g

VAC1020 EACH
EUR 84.36

Normal stomach tissue array

BN01011b each
EUR 222
Description: Normal stomach tissue array, 24 cases/72 cores, replacing BN01011

Normal bladder tissue array

BNC12011 each
EUR 222
Description: Normal bladder tissue array, including TNM, clinical stage and pathology grade, 40 cases/ 80 cores

Normal brain tissue array

BNC17011a each
EUR 306
Description: Normal brain tissue array, 25 cases/ 80 cores, replaced by BNC17011b

Normal prostate tissue array

BNS19011 each
EUR 306
Description: Normal prostate tissue array, 24 cases/ 48 cores,

Normal cerebrum tissue array

GLN241a each
EUR 198
Description: Normal cerebrum tissue array, 24 cases/24 cores, replacing GLN241

Normal heart tissue array

HEN241 each
EUR 198
Description: Normal heart tissue array, 24 cases/24 cores, replaced by HEN241a

Normal heart tissue array

HEN241a each
EUR 198
Description: Normal heart tissue array, 24 cases/24 cores (1.5mm), replacing HEN241

Normal kidney tissue array

KD803 each
EUR 354
Description: Normal kidney tissue array, replacing BN07012, 80 cases/80 cores

Normal kidney tissue array

KDN242 each
EUR 72
Description: Normal kidney tissue array, 24 cases/24 cores

Normal lung tissue array

LC725 each
EUR 270
Description: Normal lung tissue array, 24 cases/72 cores, replacing BN04011

Normal lung tissue array

LCN241 each
EUR 114
Description: Normal lung tissue array, 24 cases/24 cores

Normal liver tissue array

LVN241 each
EUR 198
Description: Normal liver tissue array, 24 cases/24 cores, replaced by LVN241a

Normal liver tissue array

LVN241a each
EUR 198
Description: Normal liver tissue array, 24 cases/24 cores, replacing LVN241

Normal spleen tissue array

SPN481 each
EUR 168
Description: Normal spleen tissue array, 24 cases/ 48 cores

Normal testis tissue array

TEN241 each
EUR 198
Description: Normal testis tissue array, 24 cases/24 cores


Collecting evidence suggests that miR-483-3p involved in maintaining the biological properties of human cancers. However, the biological role in rheumatoid arthritis (RA) remains unknown. levels of miR-483-3p in synovial tissue samples and fibroblast-like synoviocytes (FLSs) is determined by using quantitative real-time PCR. The CCK-8 assay and Edu staining was performed to assess cell proliferation in RA FLSs after transfection with miR-483-3p mimics or inhibitors. Flow cytometry with Annexin V-FITC staining or PI staining was performed to assess apoptosis or cell cycle progress in FLSs RA, respectively.

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