miR-17-5p reduces inflammation and bone erosions in collagen induced arthritis mice and directly targets the JAK-STAT pathway in rheumatoid arthritis fibroblast-like synoviocytes

miR-17-5p reduces inflammation and bone erosions in collagen induced arthritis mice and directly targets the JAK-STAT pathway in rheumatoid arthritis fibroblast-like synoviocytes

Objective: Our objective was to study miR expression in rheumatoid arthritis (RA) phenotype, along with the effects and mechanism of action of miR-17-5p.


Methods:
An array mir done in synovial tissue biopsy of n = 3 naive erosive and non-erosive RA patients. miR-17 lipoplex delivered intra-articularly in a murine model of collagen-induced arthritis. Clinical, histological and structural effects studied for arthritis. In-depth study of miR-17 mechanism-of-action is carried out in SD RA-FLS isolated from synovial tissue.


Results: 55 miRNAs including miR-17 reduced in erosive RA. miR-17 transfection at the foot of clinical rheumatoid reduces inflammation from day 2 to 7 (score 2.8 vs 1.9, p = 0.03). synovial B cells, T cells, macrophages and polynuclear neutrophils infiltrate was significantly reduced. structural damage also decreased as shown by the decrease in the number of osteoclasts (TRAP surface / total surface of 32 vs 18%, p = 0.005) and a score of erosion with the analysis of CT (score 2.9 vs. 1.7, p = 0.023). pro-inflammatory cytokines IL-6 family, and the expression of IL-1β was also reduced significantly, but not TNF. miR17 directly targeting the 3′-translated region of STAT3 and JAK1. STAT3 and JAK1 mRNA and protein expression was reduced in RA-FLS follows miR-17 transfection. STAT3 and JAK1 mRNA and activation of STAT3 assessed by immunohistochemistry was also reduced in the foot injected (% area stained 93 vs 62, p = 0.035).


Conclusions: We demonstrate the role of anti-inflammatory and anti-erosive of miR-17 in vivo. This effect involves the suppression of IL-6 autocrine loop strengthen families through direct targeting JAK1 and STAT3.

 miR-17-5p reduces inflammation and bone erosions in collagen induced arthritis mice and directly targets the JAK-STAT pathway in rheumatoid arthritis fibroblast-like synoviocytes
miR-17-5p reduces inflammation and bone erosions in collagen induced arthritis mice and directly targets the JAK-STAT pathway in rheumatoid arthritis fibroblast-like synoviocytes

The emerging role of fibroblast-like synoviocytes-mediated synovitis in osteoarthritis: Updates

Osteoarthritis (OA), a degenerative disease of the most ubiquitous affecting all the joints, characterized by cartilage degradation and synovial inflammation. Although the pathogenesis of OA is still poorly understood, synovial inflammation is known to play an important role in the development of OA. However, studies in OA pathophysiology have focused more on the degeneration of cartilage and osteophytes and not in the synovium is inflamed and thickened.

Fibroblast like synoviocytes (FLS) produces a series of pro-inflammatory regulator, such as inflammatory cytokines, nitric oxide (NO) and prostaglandin E2 (PGE2). The regulator is positively associated with clinical symptoms of OA, such as inflammatory pain, joint swelling and disease progression. A better understanding of the inflammatory immune response in OA-FLS could provide a new approach for a comprehensive strategy for the treatment of OA.

Here, we have summarized the literature recently published referring to the epigenetic modification, activated signaling pathways and related inflammatory factors involved in inflammation-mediated OA-FLS. In addition, clinical trials related to current and future perspectives are also summarized.

Human Cryopreserved Bone Cells, Normal

CDD-H-2810-N 1 vial
EUR 600

Mesenchymal Stem Cells, Mouse, Normal, Cryopreserved (mMSC)

MBS652609-500KCells 500KCells
EUR 1145

Mesenchymal Stem Cells, Mouse, Normal, Cryopreserved (mMSC)

MBS652609-5x500KCells 5x500KCells
EUR 4930

Positively Selected Normal Peripheral Blood CD19+ B Cells, Cryopreserved

PBCD19P-C10M - Ask for price

Human Cryopreserved Tendon Cells

CDD-H-2465 1 vial
EUR 575

Pooled Human Semen Cryopreserved

IRHUSMCRYP0.5ML each
EUR 1170
Description: Pooled Human Semen Cryopreserved

Pooled Human Semen Cryopreserved

MBS8421299-05mL 0.5mL
EUR 1400

Pooled Human Semen Cryopreserved

MBS8421299-5x05mL 5x0.5mL
EUR 6075

Human Cryopreserved Bone Cells, OA

CDD-H-2810-OA 1 vial
EUR 600

Human Cryopreserved Bone Cells, RA

CDD-H-2810-RA 1 vial
EUR 600

Human Cryopreserved Chondrocytes, OA

CDD-H-2610-OA 1 vial
EUR 575

Human Cryopreserved Chondrocytes, RA

CDD-H-2610-RA 1 vial
EUR 575

Human Cryopreserved Ligament Cells, ACL

CDD-H-2475-ACL 1 vial
EUR 575

Human Cryopreserved Ligament Cells, PCL

CDD-H-2475-PCL 1 vial
EUR 575

Human Synoviocytes

ABC-TC3829 1 vial Ask for price
Description: Human synoviocytes (HS), the predominant cell type of healthy synovial tissue, are fibroblast-like cells. The Synoviocytes form a distinct structure called the synovial lining layer. Electron microscopy revealed extensive cell-to-cell contacts within the lining layer. The Synoviocytes produce synovial fluid components and are responsible for absorption from the joint cavity, and for blood/synovial fluid exchanges. The synoviocytes proliferate, show anchorage-independent growth, and also secrete a variety of effector molecules that promote inflammation and joint destruction and, themselves are part of a complex network of autocrine and paracrine acting factors. The synoviocytes express cadherin-11 providing new insight into synovial tissue organization and morphogenesis and of interest as a therapeutic target.HS from Gentaur Research Laboratories are isolated from human synovium. HS are cryopreserved at passage one cultures and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HS are characterized by their fibroblast-like morphology, growth pattern and immunocytochemistry of CD 90 and fibronectin. HS are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HS are guaranteed for 15 population doublings at the conditions provided by Gentaur Research Laboratories.

Human Bone Marrow MNCs, cryopreserved, 200 million

LO2M-125A each Ask for price

Human Bone Marrow MNCs, cryopreserved, 300 million

LO2M-125E each
EUR 5588.98

Human Cryopreserved Dermal Fibroblast Cells

CDD-H-2510 1 vial
EUR 575

Human CD3+ T Cells (Pan T Cells), Cryopreserved

M1299-10 each Ask for price

Human CD3+ T Cells (Pan T Cells), Cryopreserved

M1299-20 each Ask for price

Human CD3+ T Cells (Pan T Cells), Cryopreserved

M1299-30 each Ask for price

Human CD3+ T Cells (Pan T Cells), Cryopreserved

M1299-40 each Ask for price

Human Bone Marrow MNCs, cryopreserved, 25 million

LO2M-125C each
EUR 653.68

hum. Adult Melanocytes cryopreserved ,

LOCC-2586 each
EUR 1083.07

Cryopreserved Rat Microglia cells

LOR-G-535 each
EUR 1570.82

Rat Cryopreserved Chondrocytes, P2

CDD-R-2610 1 vial
EUR 575

InEpC (Human Intestinal Epithelial Cells), cryopreserved

LOCC-2931 each
EUR 1790.15

Human Bone Marrow MNCs, cryopreserved, 5 x 25 million

LO2M-125B each
EUR 2083.13

Human Bone Marrow MNCs, cryopreserved, 5 million cells

LO2S-101D each
EUR 667.34

S-ALI guaranteed SAEC, cryopreserved

LOCC-2547S each
EUR 1969.59

Human Osteoclast Precursors, cryopreserved, 1 million cells

LO2T-110 each
EUR 2034.39

Human NK Cells (neg. selection) cryopreserved , 5 million cells

LO2W-501 each
EUR 1874.06

Human NK Cells (pos. selection) cryopreserved , 5 million cells

LO2W-502 each
EUR 1650.78

Human Fibroblast-Like Synoviocytes

cAP-0141 1Frozen Vial
EUR 693

Immortalized Human Synoviocytes-SV40

CSC-I2279Z One Frozen vial Ask for price

Human fibroblast-like synoviocytes (HFLS)

P0004018 One Frozen vial
EUR 700

Synoviocytes Growth Medium

cAP-68 500ml
EUR 148.5

Cryopreserved Rat Embryonic Dorsal Root Ganglion Neurons

LOR-EDRG-515 each
EUR 1562.97

Human Cryopreserved Intervertebral Disc Cells, Healthy Donor: Annulus Fibrosus (AF)

CDD-H-2710-AF 1 vial
EUR 575

Human Cryopreserved Intervertebral Disc Cells, Healthy Donor: Nucleus Pulposus (NP)

CDD-H-2710-NP 1 vial
EUR 575

Semen - Cryopreserved; Cryogenically Preserved Semen (Motile Sperm)

MBS173696-05mL 0.5mL
EUR 1095

Semen - Cryopreserved; Cryogenically Preserved Semen (Motile Sperm)

MBS173696-5x05mL 5x0.5mL
EUR 4720

Human Fibroblast-Like Synoviocyte Normal Tissue, Adult

ABC-TC3619 1 vial Ask for price
Description: Human Fibroblast-Like Synoviocytes (HFLS) are isolated from normal synovial tissues. They are cryopreserved at second passage and can be cultured and propagated at least 5 population doublings.

Rat Cerebellar Neurons (Granule Cells), cryopreserved, >4 million cells

LOR-CB-503 each
EUR 965.79

Human fibroblast-like synoviocytes: Rheumatoid Arthritis (HFLS-RA)

P0004019 One Frozen vial
EUR 700

Immortalized Human Rheumatoid Arthritis Fibroblast-Like Synoviocytes-hTERT

CSC-I2074Z One Frozen vial Ask for price

Epithelial Cryopreserve Media

FM301 each
EUR 259.2

Endothelial Cryopreserve Media

MBS555799-10mL 10mL
EUR 275

Endothelial Cryopreserve Media

MBS555799-5x10mL 5x10mL
EUR 1090

Human Synoviocyte Growth Medium

C0013-44 RT 500 mL Bottle
EUR 98

Cryopreservation Medium For Human Embryonic Stem Cells

HUXES-07031 20/50mL Ask for price

CRYOPRESERVATION BAG, 50ML

91-200-88 1/pk
EUR 97.2
Description: Flexible Containers; Bioprocess Bags and Parts

CRYOPRESERVATION BAG, 250ML

91-200-89 1/pk
EUR 100.8
Description: Flexible Containers; Bioprocess Bags and Parts

CRYOPRESERVATION BAG, 500ML

91-200-90 1/pk
EUR 104.4
Description: Flexible Containers; Bioprocess Bags and Parts

CRYOPRESERVATION BAG, 750ML

91-200-91 1/pk
EUR 108
Description: Flexible Containers; Bioprocess Bags and Parts

Cryopreservation Media

MR1014 1 bottle
EUR 196.65
Description: Cryopreservation Media (100mL)

Cryopreservation Medium

TM024 100ml
EUR 175

Human Fibroblast-Like Synoviocyte Osteo Arthritis, Adult

ABC-TC3620 1 vial Ask for price
Description: Human Fibroblast-Like Synoviocytes (HFLS) are isolated from synovial tissues obtained from patients with Rheumatoid Arthritis (RA) / Osteoarthritis (OA). They are cryopreserved at second passage and can be cultured and propagated at least 5 population doubling.

Serum Free Cryopreservation Medium

TM026 25 ml
EUR 50

Cryopreservation Media Kit (BRFF GTC-80)

MBS653029-1Kit 1Kit
EUR 435

Cryopreservation Media Kit (BRFF GTC-80)

MBS653029-5x1Kit 5x1Kit
EUR 1735

Waymouth Cryopreservation Medium

0459 100 ml
EUR 21.8

AddexBio Cryopreservation Medium

C0002-01-20mL RT vial
EUR 25

AddexBio Cryopreservation Medium

C0002-20mL RT vial
EUR 124.8

L7 hPSC Cryopreservation Solution, 50ml

LOFP-5002 each Ask for price

Cryopreservation Medium For General Use

CYRO-10001 20/50mL Ask for price

NCR Cryopreservation Medium For General Use

NCRC-10001 20/50mL Ask for price

Synoviocyte Growth Medium, Ready-to-Use, 500 ml, 10% Human Serum Supplement

M2700-10HS 500 ml bottle
EUR 120

Human Fibroblast-Like Synoviocyte Rheumatoid Arthritis, Adult

ABC-TC3621 1 vial Ask for price
Description: Human Fibroblast-Like Synoviocytes (HFLS) are isolated from synovial tissues obtained from patients with Rheumatoid Arthritis (RA) / Osteoarthritis (OA). They are cryopreserved at second passage and can be cultured and propagated at least 5 population doubling.

Cryopreservation Medium For Neural Stem Cells

GUXNX-07031 20/50mL Ask for price

Serum-free cell cryopreservation solution

EGQA0073 100ml
EUR 80

Synoviocyte Growth Medium, Ready-to-Use, 500 ml, 2% Human Serum Supplement Factors

M2700-2HS 500 ml bottle
EUR 120

FreezeMe Two, Cryopreservation Medium Serum-free - 50 ml

CAFM2-F each
EUR 168.68

NCR Protein-Free Cryopreservation Medium For General Use

NCPF-10001 20/50mL Ask for price

Chemical Defined Xeno-free Cell Cryopreservation Medium

abx298009-20ml 20 ml
EUR 292.8

FreezeMe One, Cryopreservation Medium with Fetal Bovine Serum - 50 ml

CAFM1-F each
EUR 168.68

QuickFreezing-I Insect cell cryopreservation solution

E28X0310043-100 100ml
EUR 361.9

NCR Protein-Free Cryopreservation Medium For Neural Stem Cells

GUXNX-07021 20/50mL Ask for price

QuickFreezing-M Mammalian cell cryopreservation solution

E28X0310041-100 100ml
EUR 184.13

QuickFreezing-M Mammalian cell cryopreservation solution

E28X0310041-30 30ml
EUR 69.84

QuickFreezing-2×M Mammalian cell cryopreservation solution

E28X0310042-100 100ml
EUR 361.9

QuickFreezing-2×M Mammalian cell cryopreservation solution

E28X0310042-30 30ml
EUR 133.33

Human Synovial Fluid, Normal

CDD-H-5000-N 0.5 ml
EUR 300

COOLCELL SV2 STEMCELL CRYOPRESERVATION SYSTEM INCLUDES THE XT STARTER BENCHTOP COOLER, COOLRACK SV2 AND COOLCELL SV2 CELL FREEZING CONTAINER FOR 12 INJECTABLE VIALS

432012 1/pk
EUR 1024.8
Description: BioCision; BioCision CoolCell

Human Synovial Tissue, Normal, FFPE

CDD-H-3000-ST-N 1 H-E stained slide, 2 unstained slides
EUR 200

Human Synovial Tissue, Fresh, Normal

CDD-H-6300-N-5G-FRESH 5g
EUR 800

Human Synovial Tissue, Normal, RNALater

CDD-H-6300-N-1G-R 1g
EUR 250

COOLCELL SV10 STEMCELL CRYOPRESERVATION SYSTEM INCLUDES THE XT STARTER BENCHTOP COOLER, COOLRACK SV10 AND COOLCELL SV10 CELL FREEZING CONTAINER FOR 6 INJECTABLE VIALS

432013 1/pk
EUR 1024.8
Description: BioCision; BioCision CoolCell

Human Synovial Tissue, Normal, OCT frozen

CDD-H-3000-OCT-ST-N Flash frozen tissue in OCT medium
EUR 250

Human Synovial Tissue, Normal, Snap Frozen

CDD-H-6300-N-1G-F 1g
EUR 250

Human Synovial Tissue Explant Culture, Normal

CDD-H-2920-N Multiwell plate format
EUR 675

Human Bone Joint PrimaCell: Normal Synovial Cell

2-96011 1 Kit Ask for price

Human Bone Joint PrimaCell: Normal Synovial Cells Growth Medium

9-46011 5 x 100 ml Ask for price

Human Bone Joint Tissue Preparation Buffer: Normal Synovial Cells

9-80011 1 x 100 ml Ask for price

Rat Bone Joint PrimaCell: Normal Synovial Cells

2-82509 1 Kit Ask for price

Mouse Bone Joint PrimaCell: Normal Synovial Cells

2-82008 1 Kit Ask for price

Human Bone Joint PrimaCell: Normal Synovial Cells Growth Supplements with Serum (for 500 ml medium)

9-47011 1 Set Ask for price

Rat Bone Joint PrimaCell: Normal Synovial Cells Growth Medium

9-25009 5 x 100 ml Ask for price

Rat Bone Joint Tissue Preparation Buffer: Normal Synovial Cell

9-80216 1 x 100 ml Ask for price

Mouse Bone Joint PrimaCell: Normal Synovial Cells Growth Medium

9-32008 5 x 100 ml Ask for price

Mouse Bone Joint Tissue Preparation Buffer: Normal Synovial Cells

9-80122 1 x 100 ml Ask for price

NHA (Normal Human Astrocytes) Cryo

LOCC-2565 each
EUR 2142.05

NHDC (Normal Human Dendritic Cells) cryo

LOCC-2701 each
EUR 1626.21

Human Synovial Tissue Explant Culture, Normal with wet weight determination provided for each explant in Excel spreadsheet

CDD-H-2920-N-WW Multiwell plate format
EUR 875

NHOst (Normal Human Osteoblasts), OGM, cryo amp

LOCC-2538 each
EUR 1240.07

NHNP (Normal Human Neural Progenitor cells), cryo

LOPT-2599 each
EUR 2396.6

Rat Bone Joint PrimaCell: Normal Synovial Cells Growth Supplements with Serum (for 500 ml medium)

9-26009 1 Set Ask for price

Mouse Bone Joint PrimaCell: Normal Synovial Cells Growth Supplements with Serum (for 500 ml medium)

9-33008 1 Set Ask for price

NHLF (Normal human Lung Fibroblasts), FGM-2 medium, cryo amp

LOCC-2512 each
EUR 1361.54

NHAC-kn (Normal Human Articular Chondrocytes), from knee, CGM, cryo amp

LOCC-2550 each
EUR 1936.08

NHDF-Ad (Adult Normal Human Dermal Fibroblasts), FGM-2 media, cryo amp

LOCC-2511 each
EUR 686.37

NHDF-Neo (Neonatal Normal Human Dermal Fibroblasts), FGM-2 media, cryo amp

LOCC-2509 each
EUR 1206.76

Normal Human Serum

90R-1002 10 ml
EUR 237
Description: Normal Human Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Human Serum

NHS 1 ml/vial
EUR 50

Serum, Human, Normal

MBS638787-2mL 2mL
EUR 410

Serum, Human, Normal

MBS638787-5x2mL 5x2mL
EUR 1700

Normal Human Serum

MBS686120-2mL 2mL
EUR 150

Normal Human Serum

MBS686120-5x2mL 5x2mL
EUR 515

Normal Human Serum

MBS686347-10mL 10mL
EUR 195

Normal Human Serum

MBS686347-5x10mL 5x10mL
EUR 735

IgM, Human, Normal Plasma

MBS654983-1mg 1mg
EUR 445

IgM, Human, Normal Plasma

MBS654983-5mg 5mg
EUR 950

IgM, Human, Normal Plasma

MBS654983-5x5mg 5x5mg
EUR 4125

Pons Lysate Normal Human

GWB-D7E80B 0.15 mg Ask for price

Normal human exosome RNA

P240 -
EUR 1499

Vomit - Normal Human Donor

MBS170483-1Sample 1Sample
EUR 730

Vomit - Normal Human Donor

MBS170483-5Samples 5Samples
EUR 2745

Vomit - Normal Human Donor

MBS170483-5x5Samples 5x5Samples
EUR 12140

Buccal Swab - Normal Human

MBS170614-1Sample 1Sample
EUR 260

Buccal Swab - Normal Human

MBS170614-5Samples 5Samples
EUR 675

Buccal Swab - Normal Human

MBS170614-5x5Samples 5x5Samples
EUR 2820

Control Normal Human IgG

MBS153737-1mg 1mg
EUR 205

Control Normal Human IgG

MBS153737-5x1mg 5x1mg
EUR 900

Human Normal Pooled Serum

HPNS 1 ml
EUR 15

Sputum, Human Donor - Normal

MBS170472-10Samples 10Samples
EUR 1815

Sputum, Human Donor - Normal

MBS170472-1Sample 1Sample
EUR 310

Sputum, Human Donor - Normal

MBS170472-5x10Samples 5x10Samples
EUR 7950

Testis (human): normal tissue

GWB-SLI234 5 slide Ask for price

Normal Human Serum (filtered)

90-1013 100 ml Ask for price
Description: Human Serum Off The Clot from Normal Donors

Medulla Lysate Normal Human

GWB-EF7ADF 0.15 mg Ask for price

Human Cartilage, Normal, FFPE

CDD-H-3000-CART-N 1 H-E stained slide, 2 unstained slides
EUR 200

Thalamus Lysate Normal Human

GWB-40F442 0.15 mg Ask for price

Human Cartilage, Fresh, Normal

CDD-H-6000-N-10G-FRESH 10g
EUR 1400

Human Cartilage, Fresh, Normal

CDD-H-6000-N-5G-FRESH 5g
EUR 800

Human Fab of normal IgG

MBS572007-10mg 10mg
EUR 880

Human Fab of normal IgG

MBS572007-5x10mg 5x10mg
EUR 3805

Human normal serum (2 ml)

AS10-1541 2 ml
EUR 139

Human normal serum (10 ml)

AS10-1542 10 ml
EUR 179

Human Fat Pad, Normal, FFPE

CDD-H-3000-FP-N 1 H-E stained slide, 2 unstained slides
EUR 200

Human Cartilage, Normal, RNALater

CDD-H-6000-N-1G-R 1g
EUR 250

Human Fat Pad, Fresh, Normal

CDD-H-6400-N-5G-FRESH 5g
EUR 800

Human Normal Tissue Blot II

1522 One blot
EUR 544.2
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are brain, heart, kidney, liver, lung, pancreas, skeletal muscle, skin, and spleen.

Human Normal Tissue Blot VI

1526 One blot
EUR 544.2
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The human normal tissues included in the blot are left cerebellum, right cerebellum, frontal lobe, occipital lobe, parietal lobe, whole eye, spinal cord, temporal lobe, and thalamus.

Human Normal Tissue Blot II

MBS153744-1Blot 1Blot
EUR 460

Human Normal Tissue Blot II

MBS153744-5x1Blot 5x1Blot
EUR 2045

Human Normal Tissue Blot VI

MBS153747-1Blot 1Blot
EUR 460

Human Normal Tissue Blot VI

MBS153747-5x1Blot 5x1Blot
EUR 2045

Human Normal Tissue Blot V

MBS153755-1Blot 1Blot
EUR 460

Human Normal Tissue Blot V

MBS153755-5x1Blot 5x1Blot
EUR 2045

Human Normal Tissue Blot I

MBS153756-1Blot 1Blot
EUR 460

Human Normal Tissue Blot I

MBS153756-5x1Blot 5x1Blot
EUR 2045

Human Normal Tissue Blot IV

MBS153757-1Blot 1Blot
EUR 460

Human Normal Tissue Blot IV

MBS153757-5x1Blot 5x1Blot
EUR 2045

Human Normal Tissue Blot III

MBS153742-1Blot 1Blot
EUR 460

Human Normal Tissue Blot III

MBS153742-5x1Blot 5x1Blot
EUR 2045

Human Fat Pad, Normal, RNALater

CDD-H-6400-N-1G-R 1g
EUR 250

Human Tissue Lysate (Normal) Human Lung

GWB-8C353C 0.15 mg Ask for price

Normal Human Epidermal Keratinocytes 

CSC-7640W One Frozen vial Ask for price

Human Normal Pooled Plasma (EDTA)

HPNPE 1 ml
EUR 15

Normal Urine, Single Human Donor

MBS170319-100Samples 100Samples
EUR 4965

Normal Urine, Single Human Donor

MBS170319-10Samples 10Samples
EUR 740

Normal Urine, Single Human Donor

MBS170319-1Sample 1Sample
EUR 210

Normal Urine, Single Human Donor

MBS170319-5x100Samples 5x100Samples
EUR 22140

Human Tissue Lysate (Normal) Human Stomach

GWB-3BB116 0.15 mg Ask for price

Tissue Lysate (Normal) Human Heart

GWB-54C253 0.15 mg Ask for price

Normal Urine, Pooled Human Donors

MBS170281-1L 1L
EUR 250

Normal Urine, Pooled Human Donors

MBS170281-5x6L 5x6L
EUR 2970

Normal Urine, Pooled Human Donors

MBS170281-6L 6L
EUR 725

Normal Sweat, Pooled Human Donors

MBS170297-100mL 100mL
EUR 4800

Normal Sweat, Pooled Human Donors

MBS170297-10mL 10mL
EUR 1065

Normal Sweat, Pooled Human Donors

MBS170297-1mL 1mL
EUR 275

Normal Sweat, Pooled Human Donors

MBS170297-5x100mL 5x100mL
EUR 21400

Plasma - Normal, Single Human Donor

MBS173495-INQUIRE INQUIRE Ask for price

Human Tissue Lysate (Normal) Human Prostate

GWB-326D8C 0.15 mg Ask for price

Human Tissue Lysate (Normal) Human Pancreas

GWB-8C6670 0.15 mg Ask for price

Tissue Lysate (Normal) Human Kidney

GWB-FEAC28 0.15 mg Ask for price

Human Cartilage, Normal, OCT frozen

CDD-H-3000-OCT-CART-N Flash frozen tissue in OCT medium
EUR 250

Normal Human IgG2 isotype control

nAP-0015 1mg
EUR 459

Plasma - Normal, Pooled Human Donors

MBS170634-INQUIRE INQUIRE Ask for price

Human Normal Pooled Plasma (Heparin)

HPNPH 1 ml
EUR 15


Collecting evidence suggests that miR-483-3p involved in maintaining the biological properties of human cancers. However, the biological role in rheumatoid arthritis (RA) remains unknown. levels of miR-483-3p in synovial tissue samples and fibroblast-like synoviocytes (FLSs) is determined by using quantitative real-time PCR. The CCK-8 assay and Edu staining was performed to assess cell proliferation in RA FLSs after transfection with miR-483-3p mimics or inhibitors. Flow cytometry with Annexin V-FITC staining or PI staining was performed to assess apoptosis or cell cycle progress in FLSs RA, respectively.

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