miR-17-5p reduces inflammation and bone erosions in collagen induced arthritis mice and directly targets the JAK-STAT pathway in rheumatoid arthritis fibroblast-like synoviocytes

miR-17-5p reduces inflammation and bone erosions in collagen induced arthritis mice and directly targets the JAK-STAT pathway in rheumatoid arthritis fibroblast-like synoviocytes

Objective: Our objective was to study miR expression in rheumatoid arthritis (RA) phenotype, along with the effects and mechanism of action of miR-17-5p.


Methods:
An array mir done in synovial tissue biopsy of n = 3 naive erosive and non-erosive RA patients. miR-17 lipoplex delivered intra-articularly in a murine model of collagen-induced arthritis. Clinical, histological and structural effects studied for arthritis. In-depth study of miR-17 mechanism-of-action is carried out in SD RA-FLS isolated from synovial tissue.


Results: 55 miRNAs including miR-17 reduced in erosive RA. miR-17 transfection at the foot of clinical rheumatoid reduces inflammation from day 2 to 7 (score 2.8 vs 1.9, p = 0.03). synovial B cells, T cells, macrophages and polynuclear neutrophils infiltrate was significantly reduced. structural damage also decreased as shown by the decrease in the number of osteoclasts (TRAP surface / total surface of 32 vs 18%, p = 0.005) and a score of erosion with the analysis of CT (score 2.9 vs. 1.7, p = 0.023). pro-inflammatory cytokines IL-6 family, and the expression of IL-1β was also reduced significantly, but not TNF. miR17 directly targeting the 3′-translated region of STAT3 and JAK1. STAT3 and JAK1 mRNA and protein expression was reduced in RA-FLS follows miR-17 transfection. STAT3 and JAK1 mRNA and activation of STAT3 assessed by immunohistochemistry was also reduced in the foot injected (% area stained 93 vs 62, p = 0.035).


Conclusions: We demonstrate the role of anti-inflammatory and anti-erosive of miR-17 in vivo. This effect involves the suppression of IL-6 autocrine loop strengthen families through direct targeting JAK1 and STAT3.

 miR-17-5p reduces inflammation and bone erosions in collagen induced arthritis mice and directly targets the JAK-STAT pathway in rheumatoid arthritis fibroblast-like synoviocytes
miR-17-5p reduces inflammation and bone erosions in collagen induced arthritis mice and directly targets the JAK-STAT pathway in rheumatoid arthritis fibroblast-like synoviocytes

The emerging role of fibroblast-like synoviocytes-mediated synovitis in osteoarthritis: Updates

Osteoarthritis (OA), a degenerative disease of the most ubiquitous affecting all the joints, characterized by cartilage degradation and synovial inflammation. Although the pathogenesis of OA is still poorly understood, synovial inflammation is known to play an important role in the development of OA. However, studies in OA pathophysiology have focused more on the degeneration of cartilage and osteophytes and not in the synovium is inflamed and thickened.

Fibroblast like synoviocytes (FLS) produces a series of pro-inflammatory regulator, such as inflammatory cytokines, nitric oxide (NO) and prostaglandin E2 (PGE2). The regulator is positively associated with clinical symptoms of OA, such as inflammatory pain, joint swelling and disease progression. A better understanding of the inflammatory immune response in OA-FLS could provide a new approach for a comprehensive strategy for the treatment of OA.

Here, we have summarized the literature recently published referring to the epigenetic modification, activated signaling pathways and related inflammatory factors involved in inflammation-mediated OA-FLS. In addition, clinical trials related to current and future perspectives are also summarized.

Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Cryopreserved

PBCD4-25-C2M - Ask for price

Positively Selected Normal Peripheral Blood CD19+ B Cells, Cryopreserved

PBCD19P-C10M - Ask for price

Human Synoviocytes

ABC-TC3829 1 vial Ask for price
Description: Human synoviocytes (HS), the predominant cell type of healthy synovial tissue, are fibroblast-like cells. The Synoviocytes form a distinct structure called the synovial lining layer. Electron microscopy revealed extensive cell-to-cell contacts within the lining layer. The Synoviocytes produce synovial fluid components and are responsible for absorption from the joint cavity, and for blood/synovial fluid exchanges. The synoviocytes proliferate, show anchorage-independent growth, and also secrete a variety of effector molecules that promote inflammation and joint destruction and, themselves are part of a complex network of autocrine and paracrine acting factors. The synoviocytes express cadherin-11 providing new insight into synovial tissue organization and morphogenesis and of interest as a therapeutic target.HS from Gentaur Research Laboratories are isolated from human synovium. HS are cryopreserved at passage one cultures and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HS are characterized by their fibroblast-like morphology, growth pattern and immunocytochemistry of CD 90 and fibronectin. HS are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HS are guaranteed for 15 population doublings at the conditions provided by Gentaur Research Laboratories.

Pooled Human Semen Cryopreserved

IRHUSMCRYP0.5ML each
EUR 1170
Description: Pooled Human Semen Cryopreserved

Human CD3+ T Cells (Pan T Cells), Cryopreserved

M1299-10 each Ask for price

Human CD3+ T Cells (Pan T Cells), Cryopreserved

M1299-20 each Ask for price

Human CD3+ T Cells (Pan T Cells), Cryopreserved

M1299-30 each Ask for price

Human CD3+ T Cells (Pan T Cells), Cryopreserved

M1299-40 each Ask for price

Human Bone Marrow CD34+ Stem Cells, Cryopreserved

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Human Bone Marrow CD34+ Stem Cells, Cryopreserved

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Human Bone Marrow CD34+ Stem Cells, Cryopreserved

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Human Peripheral Blood CD3+ T Cells, Cryopreserved

PBCD3-C10M - Ask for price

Human Peripheral Blood CD3+ T Cells, Cryopreserved

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Human Cord Blood CD34+ Cells, Cryopreserved, single donor

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Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 

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Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 

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Human Cord Blood CD34+ Cells, Cryopreserved, mixed donor 

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Human Cord Blood CD34+ Cells, Cryopreserved, single donor 

CBCD34-C1M - Ask for price

Human fibroblast-like synoviocytes (HFLS)

P0004018 One Frozen vial
EUR 700

Human  Peripheral  Blood  CD4+  T  Lymphocytes, Cryopreserved

PBCD4-C10M - Ask for price

Human  Peripheral  Blood  CD14+ Monocytes, Cryopreserved / Untouched

PBCD14U-C10M - Ask for price

Human Fibroblast-Like Synoviocyte Normal Tissue, Adult

ABC-TC3619 1 vial Ask for price
Description: Human Fibroblast-Like Synoviocytes (HFLS) are isolated from normal synovial tissues. They are cryopreserved at second passage and can be cultured and propagated at least 5 population doublings.

Human  Peripheral  Blood  CD14+ Monocytes, Cryopreserved / Positive selection

PBCD14P-C10M - Ask for price

Human fibroblast-like synoviocytes: Rheumatoid Arthritis (HFLS-RA)

P0004019 One Frozen vial
EUR 700

Endothelial Cryopreserve Media

FM300 10 ml
EUR 308.4

Human Synoviocyte Growth Medium

C0013-44 RT 500 mL Bottle
EUR 98

CRYOPRESERVATION BAG, 50ML

91-200-88 1/pk
EUR 97.2
Description: Flexible Containers; Bioprocess Bags and Parts

CRYOPRESERVATION BAG, 250ML

91-200-89 1/pk
EUR 100.8
Description: Flexible Containers; Bioprocess Bags and Parts

CRYOPRESERVATION BAG, 500ML

91-200-90 1/pk
EUR 104.4
Description: Flexible Containers; Bioprocess Bags and Parts

CRYOPRESERVATION BAG, 750ML

91-200-91 1/pk
EUR 108
Description: Flexible Containers; Bioprocess Bags and Parts

Cryopreservation Medium For Human Embryonic Stem Cells

HUXES-07031 20/50mL Ask for price

Cryopreservation Medium

TM024 100ml
EUR 175

Human Fibroblast-Like Synoviocyte Osteo Arthritis, Adult

ABC-TC3620 1 vial Ask for price
Description: Human Fibroblast-Like Synoviocytes (HFLS) are isolated from synovial tissues obtained from patients with Rheumatoid Arthritis (RA) / Osteoarthritis (OA). They are cryopreserved at second passage and can be cultured and propagated at least 5 population doubling.

Serum Free Cryopreservation Medium

TM026 25 ml
EUR 50

AddexBio Cryopreservation Medium

C0002-20mL RT vial
EUR 124.8

AddexBio Cryopreservation Medium

C0002-01-20mL RT vial
EUR 25

Human Fibroblast-Like Synoviocyte Rheumatoid Arthritis, Adult

ABC-TC3621 1 vial Ask for price
Description: Human Fibroblast-Like Synoviocytes (HFLS) are isolated from synovial tissues obtained from patients with Rheumatoid Arthritis (RA) / Osteoarthritis (OA). They are cryopreserved at second passage and can be cultured and propagated at least 5 population doubling.

Cryopreservation Medium For General Use

CYRO-10001 20/50mL Ask for price

NCR Cryopreservation Medium For General Use

NCRC-10001 20/50mL Ask for price

Cryopreservation Medium For Neural Stem Cells

GUXNX-07031 20/50mL Ask for price

Serum-free cell cryopreservation solution

EGQA0073 100ml
EUR 80

NCR Protein-Free Cryopreservation Medium For General Use

NCPF-10001 20/50mL Ask for price

Chemical Defined Xeno-free Cell Cryopreservation Medium

abx298009-20ml 20 ml
EUR 292.8

NCR Protein-Free Cryopreservation Medium For Neural Stem Cells

GUXNX-07021 20/50mL Ask for price

QuickFreezing-I Insect cell cryopreservation solution

E28X0310043-100 100ml
EUR 361.9

QuickFreezing-M Mammalian cell cryopreservation solution

E28X0310041-100 100ml
EUR 184.13

QuickFreezing-M Mammalian cell cryopreservation solution

E28X0310041-30 30ml
EUR 69.84

QuickFreezing-2×M Mammalian cell cryopreservation solution

E28X0310042-100 100ml
EUR 361.9

QuickFreezing-2×M Mammalian cell cryopreservation solution

E28X0310042-30 30ml
EUR 133.33

COOLCELL SV2 STEMCELL CRYOPRESERVATION SYSTEM INCLUDES THE XT STARTER BENCHTOP COOLER, COOLRACK SV2 AND COOLCELL SV2 CELL FREEZING CONTAINER FOR 12 INJECTABLE VIALS

432012 1/pk
EUR 1024.8
Description: BioCision; BioCision CoolCell

COOLCELL SV10 STEMCELL CRYOPRESERVATION SYSTEM INCLUDES THE XT STARTER BENCHTOP COOLER, COOLRACK SV10 AND COOLCELL SV10 CELL FREEZING CONTAINER FOR 6 INJECTABLE VIALS

432013 1/pk
EUR 1024.8
Description: BioCision; BioCision CoolCell

Human Bone Joint PrimaCell: Normal Synovial Cell

2-96011 1 Kit Ask for price

Human Bone Joint PrimaCell: Normal Synovial Cells Growth Medium

9-46011 5 x 100 ml Ask for price

Human Bone Joint Tissue Preparation Buffer: Normal Synovial Cells

9-80011 1 x 100 ml Ask for price

Rat Bone Joint PrimaCell: Normal Synovial Cells

2-82509 1 Kit Ask for price

Mouse Bone Joint PrimaCell: Normal Synovial Cells

2-82008 1 Kit Ask for price

Rat Bone Joint PrimaCell: Normal Synovial Cells Growth Medium

9-25009 5 x 100 ml Ask for price

Rat Bone Joint Tissue Preparation Buffer: Normal Synovial Cell

9-80216 1 x 100 ml Ask for price

Mouse Bone Joint PrimaCell: Normal Synovial Cells Growth Medium

9-32008 5 x 100 ml Ask for price

Mouse Bone Joint Tissue Preparation Buffer: Normal Synovial Cells

9-80122 1 x 100 ml Ask for price

Human Bone Joint PrimaCell: Normal Synovial Cells Growth Supplements with Serum (for 500 ml medium)

9-47011 1 Set Ask for price

Rat Bone Joint PrimaCell: Normal Synovial Cells Growth Supplements with Serum (for 500 ml medium)

9-26009 1 Set Ask for price

Mouse Bone Joint PrimaCell: Normal Synovial Cells Growth Supplements with Serum (for 500 ml medium)

9-33008 1 Set Ask for price

Normal Human Serum

90R-1002 10 ml
EUR 304.8
Description: Normal Human Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal human exosome RNA

P240 - Ask for price

Human Normal Pooled Serum

HPNS 1 ml
EUR 15

Normal Human Serum (filtered)

90-1013 100 ml
EUR 657.6
Description: Human Serum Off The Clot from Normal Donors

Human Normal Tissue Blot II

1522 One blot
EUR 544.2
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are brain, heart, kidney, liver, lung, pancreas, skeletal muscle, skin, and spleen.

Human Normal Tissue Blot VI

1526 One blot
EUR 544.2
Description: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 µg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The human normal tissues included in the blot are left cerebellum, right cerebellum, frontal lobe, occipital lobe, parietal lobe, whole eye, spinal cord, temporal lobe, and thalamus.

Human Normal Pooled Plasma (EDTA)

HPNPE 1 ml
EUR 15

Universal RNA - Human Normal Tissues

R4234565 4x100 ug
EUR 766

Universal RNA - Human Normal Tissues

R4234565-1 2x100 ug
EUR 450

Human Normal Pooled Plasma (Heparin)

HPNPH 1 ml
EUR 15

Human synovial apoptosis inhibitor 1, synoviolin(SYVN1) ELISA Kit

NSL2294Hu 96 wells
EUR 468

HighQC™ Human IPS Cell (Normal)

ABC-SC0001T 1 vial Ask for price
Description: Induced Pluriopotent Stem Cells (iPSCs) are a type of pluripotent stem cells that can be derived directly from adult somatic cells . The derived iPSCs can propagate indefinitely, as well as give rise to other cell types in the body. iPS cells, thus, hold great promise in the field of regenerative medicine by representing a single source of cells that could be used to replace those damaged/diseased cells.

Normal Human Primary Lung Fibroblast

T5516 5x10^5 cells / 1.0 ml
EUR 725

Normal human respiratory tract larynx

RS321 each
EUR 306
Description: Normal human respiratory tract larynx, trachea, bronchi and lung tissue array, 32 cases/32 cores, 2.0 mm core size.

Normal Human Primary Kidney Podocytes

T5519 2x10^5 cells / 1.0 ml Ask for price

Normal human tissues, 19 organs (2mm)

MNO381 1
EUR 228
Description: Normal tissues from 19 anatomic sites duplicates from 38 individuals, most frequently used for tissue profiling.

Total RNA - Human Tumor Tissue: Synoviosarcoma

R1235257-10 10 ug
EUR 400

Human Bone PrimaCell: Normal Osteoblasts

2-96012 1 Kit Ask for price

Human Normal Peripheral Blood Basophils

PBB-F03M 0.3 million
EUR 1228.8

cDNA - Human Adult Normal Tissue: Lung

C1234152 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Skin

C1234218 40 reactions
EUR 385

Human Fat PrimaCell2: Normal Preadipocyte

2-96063 1 Kit Ask for price

Human Skin PrimaCell2: Normal Melanocytes

2-96102 1 Kit Ask for price

cDNA - Human Adult Normal Tissue: Brain

C1234035 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Colon

C1234090 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Heart

C1234122 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Liver

C1234149 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Ovary

C1234183 40 reactions
EUR 1049

cDNA - Human Adult Normal Tissue: Theca

C1234261-10 10 reactions
EUR 215

Human Normal Peripheral Blood Eosinophils

PBE-F1M 1 million
EUR 1210.8

cDNA - Human Adult Normal Tissue: Breast

C1234086 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Kidney

C1234142 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Rectum

C1234206 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Spleen

C1234246 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Testis

C1234260 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Throat

C1234263-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Thymus

C1234264 40 reactions
EUR 1049

cDNA - Human Adult Normal Tissue: Tongue

C1234267-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Tonsil

C1234268-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Uterus

C1234274 40 reactions
EUR 385

Normal Human Primary Plateable Hepatocytes

T5539 1x10^6 cells / 1.0 ml
EUR 925

Normal Human Primary Plateable Hepatocytes

T5541 1x10^6 cells / 1.0 ml Ask for price

cDNA - Human Adult Normal Tissue: Adipose

C1234003-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Adrenal

C1234004 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Bladder

C1234010 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Trachea

C1234160-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Stomach

C1234248 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Thyroid

C1234265 40 reactions
EUR 385

Normal Human Primary Suspension Hepatocytes

T5538 1x10^6 cells / 1.0 ml Ask for price

cDNA - Human Adult Normal Tissue: Pancreas

C1234188 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Placenta

C1234200 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Prostate

C1234201 40 reactions
EUR 385

Human Normal Peripheral Blood Leuko Pak

FP-001-1 Quarter Pak (1/4 bag)
EUR 2158.8

Human Normal Peripheral Blood Leuko Pak

FP-001-2 Half Pak (1/2 bag)
EUR 2347.2

Human Normal Peripheral Blood Leuko Pak

FP-001-3 Full Pak (1 bag)
EUR 3478.8

Human Lung Slide (Normal) (5 slides/pk)

HTS-10101 1 pk
EUR 343.2

Total Protein - Human Tumor Tissue: Synoviosarcoma

P1235257 1 mg
EUR 471

cDNA - Human Adult Normal Tissue: Esophagus

C1234106 40 reactions
EUR 385

Human Liver Slide (Normal) (5 slides/pk)

HTS-10201 1 pk
EUR 343.2

Human Brain Slide (Normal) (5 slides/pk)

HTS-10301 1 pk
EUR 343.2

Human Heart Slide (Normal) (5 slides/pk)

HTS-10501 1 pk
EUR 343.2

Human Fat PrimaCell1: Normal Adipose Cells

2-96062 1 Kit Ask for price

Human Normal Pooled Plasma (Sodium Citrate)

HPNPC 1 ml
EUR 15

Normal human tissues, FDA, 33 organs (1.5mm)

MNO341 1
EUR 154.8
Description: 33 types of normal tissues based on the FDA recommendation for antibody cross-reactivity testing.

Human Breast Slide (Normal) (5 slides/pk)

HTS-10001 1 pk
EUR 343.2

Human Kidney Slide (Normal) (5 slides/pk)

HTS-10401 1 pk
EUR 343.2

Human Cervix Slide (Normal) (5 slides/pk)

HTS-11301 1 pk
EUR 343.2

Human Brain PrimaCell10: Normal Neuron Cell

2-96022 1 Kit Ask for price

Normal Human Small Airway Epithelial Cells

T5514 5x10^5 cells / 1.0 ml
EUR 875

Normal Human Primary Kidney Cortical Cells

T5517 5x10^5 cells / 1.0 ml
EUR 875

Normal Human Primary Hepatic Kupffer Cells

T5549 1x10^6 cells / 1.0 ml
EUR 1115

Human Bladder Slide (Normal) (5 slides/pk)

HTS-12601 1 pk
EUR 343.2

Normal kidney and normal adjacent kidney tissue array

KDN241a each
EUR 138
Description: Normal kidney and normal adjacent kidney tissue array, 24 cases/ 24 cores

Human Testis PrimaCell: Normal Sertoli Cells

2-96105 1 Kit Ask for price

Normal Human Primary Kidney Mesangial Cells

T5521 5x10^5 cells / 1.0 ml
EUR 875

Normal Human Primary Hepatic Stellate Cells

T5546 1x10^6 cells / 1.0 ml
EUR 1175

cDNA - Human Adult Normal Tissue: Whole Eye

C1234108-10 10 reactions
EUR 468

Total RNA - Human Adult Normal Tissue: Lung

R1234152-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Skin

R1234218-50 50 ug
EUR 284

Human Skin PrimaCell: Normal Skin Fibroblasts

2-96103 1 Kit Ask for price

Human Normal Peripheral Blood CD14+ Monocytes

PBCD14P-C20M 20 million
EUR 1114.8

Human Normal Peripheral Blood CD14+ Monocytes

PBCD14P-C40M 40 million
EUR 1614

Human Normal Peripheral Blood CD14+ Monocytes

PBCD14P-F20M 20 million
EUR 1402.8

Human Normal Peripheral Blood CD14+ Monocytes

PBCD14P-F40M 40 million
EUR 1678.8

cDNA - Human Adult Normal Tissue: Brain: Pons

C1234071-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Eye: Retina

C1234110-10 10 reactions
EUR 468

cDNA - Human Adult Normal Tissue: Lymph Node

C1234161-10 10 reactions
EUR 215

Total RNA - Human Adult Normal Tissue: Brain

R1234035-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Cecum

R1234089-10 10 ug
EUR 183

Total RNA - Human Adult Normal Tissue: Colon

R1234090-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Heart

R1234122-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Liver

R1234149-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Ovary

R1234183-10 10 ug
EUR 643

Total RNA - Human Adult Normal Tissue: Penis

R1234194-50 50 ug
EUR 170

Human Brain PrimaCell7: Normal Meningeal Cells

2-96019 1 Kit Ask for price

Human Brain PrimaCell9: Normal Nerve Microglia

2-96021 1 Kit Ask for price

Human Heart PrimaCell2: Normal Atrial Myocytes

2-96067 1 Kit Ask for price

Human Lung PrimaCell1: Normal Lung Fibroblasts

2-96081 1 Kit Ask for price

Universal Protein Lysate: Human Normal Tissues

P4234565 4x0.5 mg
EUR 405

Universal Protein Lysate: Human Normal Tissues

P4234565-1 2x0.5 mg
EUR 270

cDNA - Human Adult Normal Tissue: Bone Marrow

C1234024-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Spinal Cord

C1234234 40 reactions
EUR 385

Human Adult Eye whole (Normal) tissue lysate

HAL-1385 100 ug
EUR 285.6

Human Colorectal Slide (Normal) (5 slides/pk)

HTS-10701 1 pk
EUR 343.2

Total RNA - Human Adult Normal Tissue: Breast

R1234086-50 50 ug
EUR 284

Total RNA - Human Adult Normal Tissue: Kidney

R1234142-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Rectum

R1234206-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Spleen

R1234246-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Testis

R1234260-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Throat

R1234263-10 10 ug
EUR 191

Total RNA - Human Adult Normal Tissue: Thymus

R1234264-10 10 µg
EUR 643

Total RNA - Human Adult Normal Tissue: Tongue

R1234267-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Tonsil

R1234268-10 10 ug
EUR 191

Total RNA - Human Adult Normal Tissue: Uterus

R1234274-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Vagina

R1234283-10 10 ug
EUR 191

Human Brain PrimaCell8: Normal Nerve Astrocytes

2-96020 1 Kit Ask for price

Human Kidney PrimaCell6: Normal Renal Podocytes

2-96079 1 Kit Ask for price

Human Normal Apheresis Peripheral Blood Plasma

FP-003-10 10 mL
EUR 114

Human Normal Apheresis Peripheral Blood Plasma

FP-003-100 100 mL
EUR 478.8

Human Normal Apheresis Peripheral Blood Plasma

FP-003-20 20 mL
EUR 154.8

Human Normal Apheresis Peripheral Blood Plasma

FP-003-30 30 mL
EUR 201.6

Human Normal Apheresis Peripheral Blood Plasma

FP-003-40 40 mL
EUR 253.2

Human Normal Apheresis Peripheral Blood Plasma

FP-003-50 50 mL
EUR 274.8

Normal Human Primary Broncial Epithelial Cells

T5511 5x10^5 cells / 1.0 ml
EUR 875

Normal Human Primary Tracheal Epithelial Cells

T5512 5x10^5 cells / 1.0 ml
EUR 875

Normal Human Primary Tracheal Epithelial Cells

T5513 5x10^5 cells / 1.0 ml
EUR 875

Human Adult Lung (Normal) Whole tissue lysate

HAL-1302 1 mg
EUR 628.8


Collecting evidence suggests that miR-483-3p involved in maintaining the biological properties of human cancers. However, the biological role in rheumatoid arthritis (RA) remains unknown. levels of miR-483-3p in synovial tissue samples and fibroblast-like synoviocytes (FLSs) is determined by using quantitative real-time PCR. The CCK-8 assay and Edu staining was performed to assess cell proliferation in RA FLSs after transfection with miR-483-3p mimics or inhibitors. Flow cytometry with Annexin V-FITC staining or PI staining was performed to assess apoptosis or cell cycle progress in FLSs RA, respectively.

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