We have previously reported successful insulation and cryopreservation of human intestinal mucosa (CHIM) with the viability retention and activity of drug metabolism enzymes. Here we report the results of quantification of drug metabolism activities in Chim from various regions in the small intestine of 14 individual donors. Chim isolated from the duodenum, jejunum, and ileum of 10 individuals, and from 10 consecutive 12-inch segments ranging from pyloric sfingter from the small human intestine of four additional individuals.

P450 and non-P450 medicine metabolization enzymes (CYP1A2, CYP2C8, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1, CYP3A, UGT, Sust, AO, NAT1, and NAT2) are measured through selective substrate incubation. Activities that can be measured are observed for all lanes except for CYP2A6. Comparison of duodenal, jejunum, and ileum in 10 donors showing Jejunum has a higher activity for CYP2C9, CYP3A, UGT, SULT, MAO, and NAT1. More definitions of regional variations with Chim from ten proximal small intestinal segments show that the segment immediately after the first 12-inch segment (duodenum) has the highest activity for most drug metabolism enzymes but with a substantial difference in four donors. Our overall results show that there are substantial individual differences in drug metabolism enzymes and that Jejunum, especially the region as soon as the duodenum, has the highest drug metabolic enzyme activity.

The effects of leukocyte anti-human antigen immunity sensitization from allographic cryopreserved and blood transfusions

Allotransplansis of vascular composite (VCA) is the most sophisticated reconstructive technique available for patients who suffer devastating burns into legs or faces. However, VCA requires matching donors. Burn patients have reportedly experienced sensitization, or the development of anti-human antigen antigens leukocyte, during resuscitation and wound coverage, potentially blocking them from future VCA. This study strives to investigate blood and allographical contributions to sensitization in burn patients. Four groups compared: burns of burns that accept blood products and allograft (group 1), burn patients who only accept blood products (group 2), trauma patients only accept blood products (group 3), and healthy volunteer control (Group 4). The average calculation of antibodies of the reactive panel (shows sensitization) is higher in group 1 compared to group 4 (p = 0.035).

In addition, the incidence of severe severe sensitization in group 1 was relative to other groups (p = 0.049). When comparing groups of patients who do not have sensitization, minor sensitization, moderate sensitization, and severe sensitization, there is no significant difference in age, gender, blood products received, the total body surface area burned, or allograft used between groups, though Very sensitive patients tend to have the involvement of a larger body surface area and receive more packaged red blood cell units and allograft (p = 0.079, p = 0.196, and p = 0.072, respectively). Therefore we conclude that while burning patients who receive allograft and blood show a higher incidence of the sensitization of leukocyte anti-human antigens relative to healthy control, this difference cannot be only associated with the use of allograft or transfusions.

Application of Cryopreserved Human Intestinal Mucosa and Human Enterocyt CryoPreserved in the Evaluation of Herb-Drug Interaction: Evaluation of the Potential inhibition of CYP3A Juice Balinese Juice and Twenty-Nine Commercial Formulation Herbal Supplements

Commercial Formulations 29 Herbal supplements commonly used (HS) and grapefruit juice are evaluated for potential drug interactions through the quantification of their CYP3A inhibition potential in two experimental models in vitro from the small intestine of humans, cryopreserved human intestinal mucosa and the Human Enterocyte CryoPreserved (CHE). Two CYP3A substrates are used – in studies with Chim, CYP3A activity is quantified through the quantification of LC / MS MSZolam 1′-hydroxylation while in CHE, Luciferin Science metabolism to Luciferin is quantified by luminescence.

After Chim’s treatment with the estimated concentration of HS lumen in every oral administration (the suggested dose was dissolved in 200 ml of culture media),> 80% inhibition of CYP3A was observed for green tea extract, Valeria roots, St. John, horehound juice and grapefruit. Obstacles of less than 50% observed for fenugreek, aloe vera, guarana, soybean isoflavone, maca, echinacea, spirulina, evening primrose, milk thistle, cranberry, ria yeast, rhodiola, ginkgo biloba, turmeric, curcumin, white beans, garlic, garlic, Cinnamon, see palmetto berry fruit, panax ginseng, black grass juice, wheat grass juice, flaxseed oil, black cohosh, and ginger root. The results are confirmed in the study of dose-responses with HS obtained from three suppliers for four HS inhibition (green tea extract, horehound, St. John’s Wort, Root Valerian), and three HS Black Elderberry (Black Cohosh, Black Elderberry), Echinacea).

Similar results were obtained with HS inhibitors in Che. The results illustrate that Chim and Che represent an experimental model in vitro which is physiologically relevant to evaluate the potential for herbal supplements drug interactions. Significance Statement in Evaluation in Vitro 29 Popular Herbal Supplements in Cryopreserved Human Intestinal Mucosa Identifying Green Tea Extract, Horehound, St. John’s Wort, Root Valerian to have the potential for inhibition of CYP3A similar to grapefruit juice, indicates their potential to have a clinically significant pharmacokinetics. Interaction with orally managed drugs is the CYP3A substrate. The results showed that Chim could be used for evaluating in vitro drug interactions involving enteric drug metabolism.

Extract Vulgare Origanum Aqueous improves the quality of human spermatozoa cryopreserved through its antioxidant effects

Excessive production of reactive oxygen species (ROS) during cement cryopreservation can cause structural and functional changes in spermatozoa. It is known that antioxidants can reduce the ROS effect. In addition, the application of antioxidants in media freezing is the right strategy to protect spermatozoa to the adverse effects of ROS during the cryopreservation process. For example, Oregano is a medicinal plant with important activities, with antiseptic properties, antibacterial, antitrombotic, and antioxidants.

Human Airway PrimaCell2: Normal Bronchial and Tracheal Epithelial Cells Growth Medium
9-46002	CHI Scientific	5 x 100 ml	Ask for price

Immortalized Human Bronchial Smooth Muscle Cells
T0493	ABM	1x106 cells / 1.0 ml	Ask for price

Human Airway Tissue Preparation Buffer 2: Normal Bronchial and Tracheal Epithelial Cells
9-80002	CHI Scientific	1 x 100 ml	Ask for price

Rat Airway PrimaCell1: Normal Bronchial and Tracheal Epithelial Cells Growth Medium
9-25001	CHI Scientific	5 x 100 ml	Ask for price

Mouse Airway PrimaCell1: Normal Bronchial and Tracheal Epithelial Cells Growth Medium
9-32001	CHI Scientific	5 x 100 ml	Ask for price

Mouse Airway Tissue Preparation Buffer 1: Normal Bronchial and Tracheal Epithelial Cells
9-80115	CHI Scientific	1 x 100 ml	Ask for price

Rat Airway Tissue Preparation Buffer 1: Normal Bronchial and Tracheal Epithelial Cells
9-80208	CHI Scientific	1 x 100 ml	Ask for price

Immortalized Human Mammary Epithelial Cells
T0450	ABM	1x106 cells / 1.0 ml	EUR 1243.2

Immortalized Human Lung Epithelial Cells
T0488	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Tracheal Epithelial Cells
T0489	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Corneal Epithelial Cells
T0572	ABM	1x106 cells / 1.0 ml	EUR 895.2

Immortalized Human Prostate Epithelial Cells
T0600	ABM	1x106 cells / 1.0 ml	EUR 756

Immortalized Human Renal Epithelial Cells
T0616	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Urethral Epithelial Cells
T0629	ABM	1x106 cells / 1.0 ml	Ask for price

Fibroblasts/Epithelial Cells Antibody
abx413035-02mg	Abbexa	0.2 mg	EUR 777.6

Fibroblasts/Epithelial Cells Antibody
abx413036-25ug	Abbexa	25 ug	EUR 360

Retinal Pigment Epithelial Cells
RPE001	Neuromics	500,000 Cells	EUR 1545.6

Human Bronchial CAFs
CAF111	Neuromics	1,000,000 cells	Ask for price

Human Airway PrimaCell2: Normal Bronchial and Tracheal Epithelial Cells Growth Supplements with Serum (for 500 ml medium)
9-47002	CHI Scientific	1 Set	Ask for price

Human Airway PrimaCell3: Normal Bronchial and Tracheal Smooth Muscle Cells
2-96003	CHI Scientific	1 Kit	Ask for price

Human Thyroid PrimaCell: Thyroid Epithelial Cells
2-96124	CHI Scientific	1 Kit	Ask for price

Human Renal Proximal Tubule Epithelial Cells
SC00A1-PTEC	Neuromics	500,000 Cells	EUR 1585.2

Immortalized Human Pterygium Epithelial Cells - SV40
T0360	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Conjuctival Epithelial Cells - SV40
T0361	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Nasal Epithelial Cells - SV40
T0440	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Placental Epithelial Cells - SV40
T0530	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Amniotic Epithelial Cells - SV40
T0531	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Retinal Pigment Epithelial Cells
T0571	ABM	1x106 cells / 1.0 ml	EUR 756

Immortalized Human Lens Epithelial Cells - SV40
T0573	ABM	1x106 cells / 1.0 ml	EUR 895.2

Immortalized Human Renal Cortical Epithelial Cells
T0614	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Renal Medullary Epithelial Cells
T0615	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Nasopharyngeal Epithelial Cells (NP69SV40T)
T0634	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Ovarian Epithelial Cells - SV40
T1074	ABM	1x106 cells / 1.0 ml	Ask for price

Fibroblasts/Epithelial Cells Antibody (RPE)
abx413037-100tests	Abbexa	100 tests	EUR 811.2

Fibroblasts/Epithelial Cells Antibody (RPE)
abx413038-25tests	Abbexa	25 tests	EUR 427.2

Rat Airway PrimaCell1: Normal Bronchial and Tracheal Epithelial Cells Growth Supplements with Serum (for 500 ml medium)
9-26001	CHI Scientific	1 Set	Ask for price

Mouse Airway PrimaCell1: Normal Bronchial and Tracheal Epithelial Cells Growth Supplements with Serum (for 500 ml medium)
9-33001	CHI Scientific	1 Set	Ask for price

Mouse Airway PrimaCell2: Normal Bronchial and Tracheal Smooth Muscle Cells
2-82002	CHI Scientific	1 Kit	Ask for price

Rat Airway PrimaCell2: Normal Bronchial and Tracheal Smooth Muscle Cells
2-82502	CHI Scientific	1 Kit	Ask for price

Human Airway PrimaCell1: Normal Airway Epithelial Cells
2-96001	CHI Scientific	1 Kit	Ask for price

Human Bladder PrimaCell1: Normal Bladder Epithelial Cells
2-96007	CHI Scientific	1 Kit	Ask for price

Human Cervix PrimaCell1: Normal Cervical Epithelial Cells
2-96044	CHI Scientific	1 Kit	Ask for price

Human Cervix PrimaCell4: Normal Endometrial Epithelial Cells
2-96047	CHI Scientific	1 Kit	Ask for price

Human Esophagus PrimaCell1: Normal Esophageal Epithelial Cells
2-96055	CHI Scientific	1 Kit	Ask for price

Human Eye PrimaCell2: Normal Corneal Epithelial Cells
2-96058	CHI Scientific	1 Kit	Ask for price

Human Eye PrimaCell4: Normal Lens Epithelial Cells
2-96060	CHI Scientific	1 Kit	Ask for price

Human Kidney PrimaCell2: Normal Kidney Epithelial Cells
2-96075	CHI Scientific	1 Kit	Ask for price

Human Ovary PrimaCell1: Normal Ovarian Epithelial Cells
2-96094	CHI Scientific	1 Kit	Ask for price

Human Prostate PrimaCell: Normal Prostate Epithelial Cells
2-96099	CHI Scientific	1 Kit	Ask for price

Human Ureter PrimaCell2: Normal Ureter Epithelial Cells
2-96112	CHI Scientific	1 Kit	Ask for price

Human Urethral PrimaCell: Normal Urethral Epithelial Cells
2-96113	CHI Scientific	1 Kit	Ask for price

Human Colon PrimaCell: Normal Colorectal Epithelial Cells
2-96115	CHI Scientific	1 Kit	Ask for price

Human Pancreas PrimaCell 2: Pancreatic Epithelial Cells
2-96123	CHI Scientific	1 Kit	Ask for price

Immortalized Human Mammary MUC+ESA+ Epithelial Cells
T0453	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Mammary Epithelial Cells (HMEC 2.6)
T0454	ABM	1x106 cells / 1.0 ml	EUR 1243.2

Immortalized Human Small Airway Epithelial Cells - SV40
T0485	ABM	1x106 cells / 1.0 ml	EUR 1243.2

Immortalized Human Esophageal Epithelial Cells (NE2-hTERT)
T0632	ABM	1x106 cells / 1.0 ml	EUR 1243.2

Immortalized Human Nasopharyngeal Epithelial Cells (NPEC1/Bmi1)
T0765	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Nasopharyngeal Epithelial Cells (NPEC2/Bmi1)
T0766	ABM	1x106 cells / 1.0 ml	Ask for price

Epithelial Dissociation System 11 (Epithelial Cells), Mouse and Rat
4-20261	CHI Scientific	ea	Ask for price

Human Airway PrimaCell3: Normal Bronchial and Tracheal Smooth Muscle Cells Growth Medium
9-46003	CHI Scientific	5 x 100 ml	Ask for price

Mouse Thyroid PrimaCell: Thyroid Epithelial Cells
2-82103	CHI Scientific	1 Kit	Ask for price

Rat Thyroid PrimaCell: Thyroid Epithelial Cells
2-82598	CHI Scientific	1 Kit	Ask for price

Immortalized Mouse Mammary Epithelial Cells (iMMEC)
T0451	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Mouse Prostate Epithelial Cells (iMPEC)
T0607	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Ovarian Surface Epithelial Cells (iMOSEC)
T0608	ABM	1x106 cells / 1.0 ml	Ask for price

Human Breast PrimaCell: Normal Mammary Epithelial Primary Cells
2-96023	CHI Scientific	1 Kit	Ask for price

Human Eye PrimaCell5: Normal Retinal Pigment Epithelial Cells
2-96061	CHI Scientific	1 Kit	Ask for price

Human Intestine PrimaCell1: Normal Intestinal Mucosa Epithelial Cells
2-96071	CHI Scientific	1 Kit	Ask for price

Human Kidney PrimaCell7: Normal Renal Tubular Epithelial Cells
2-96080	CHI Scientific	1 Kit	Ask for price

Human Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells
2-96104	CHI Scientific	1 Kit	Ask for price

Human Lung PrimaCell 7: Alveolar Epithelial Cells II
2-96122	CHI Scientific	1 Kit	Ask for price

Immortalized Human Mammary Epithelial Cells (HMEC 2.6) - SV40
T0455	ABM	1x106 cells / 1.0 ml	EUR 1243.2

Immortalized Human Tonsillar Epithelial Cells (E6/E7 HTE)
T0660	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Cystic Fibrosis Tracheobronchial Epithelial Cells (CFT1)
T0681	ABM	1x106 cells / 1.0 ml	EUR 686.4

Human Airway Tissue Preparation Buffer 3: Normal Bronchial and Tracheal Smooth Muscle Cells
9-80003	CHI Scientific	1 x 100 ml	Ask for price

Rat Airway PrimaCell2: Normal Bronchial and Tracheal Smooth Muscle Cells Growth Medium
9-25002	CHI Scientific	5 x 100 ml	Ask for price

Mouse Airway PrimaCell2: Normal Bronchial and Tracheal Smooth Muscle Cells Growth Medium
9-32002	CHI Scientific	5 x 100 ml	Ask for price

Mouse Bladder PrimaCell1: Normal Bladder Epithelial Cells
2-82006	CHI Scientific	1 Kit	Ask for price

Mouse Cervix PrimaCell1: Normal Cervical Epithelial Cells
2-82023	CHI Scientific	1 Kit	Ask for price

Mouse Cervix PrimaCell4: Normal Endometrial Epithelial Cells
2-82026	CHI Scientific	1 Kit	Ask for price

Mouse Colon PrimaCell: Normal Colorectal Epithelial Cells
2-82028	CHI Scientific	1 Kit	Ask for price

Mouse Esophagus PrimaCell1: Normal Esophageal Epithelial Cells
2-82035	CHI Scientific	1 Kit	Ask for price

Mouse Eye PrimaCell2: Normal Corneal Epithelial Cells
2-82038	CHI Scientific	1 Kit	Ask for price

Mouse Eye PrimaCell4: Normal Lens Epithelial Cells
2-82040	CHI Scientific	1 Kit	Ask for price

Mouse Kidney PrimaCell2: Normal Kidney Epithelial Cells
2-82057	CHI Scientific	1 Kit	Ask for price

Mouse Ovary PrimaCell1: Normal Ovarian Epithelial Cells
2-82077	CHI Scientific	1 Kit	Ask for price

Mouse Prostate PrimaCell: Normal Prostate Epithelial Cells
2-82083	CHI Scientific	1 Kit	Ask for price

Mouse Ureter PrimaCell: Normal Ureter Epithelial Cells
2-82092	CHI Scientific	1 Kit	Ask for price

Mouse Urethral PrimaCell: Normal Urethral Epithelial Cells
2-82093	CHI Scientific	1 Kit	Ask for price

Mouse Pancreas PrimaCell 2: Pancreatic Epithelial Cells
2-82102	CHI Scientific	1 Kit	Ask for price

Rat Bladder PrimaCell1: Normal Bladder Epithelial Cells
2-82506	CHI Scientific	1 Kit	Ask for price

Rat Cervix PrimaCell1: Normal Cervical Epithelial Cells
2-82524	CHI Scientific	1 Kit	Ask for price

Rat Cervix PrimaCell4: Normal Endometrial Epithelial Cells
2-82527	CHI Scientific	1 Kit	Ask for price

Rat Colon PrimaCell1: Normal Colorectal Epithelial Cells
2-82529	CHI Scientific	1 Kit	Ask for price

Rat Esophagus PrimaCell1: Normal Esophageal Epithelial Cells
2-82536	CHI Scientific	1 Kit	Ask for price

Rat Eye PrimaCell2: Normal Corneal Epithelial Cells
2-82539	CHI Scientific	1 Kit	Ask for price

Rat Eye PrimaCell4: Normal Lens Epithelial Cells
2-82541	CHI Scientific	1 Kit	Ask for price

Rat Kidney PrimaCell2: Normal Kidney Epithelial Cells
2-82556	CHI Scientific	1 Kit	Ask for price

Rat Ovary PrimaCell1: Normal Ovarian Epithelial Cells
2-82576	CHI Scientific	1 Kit	Ask for price

Rat Prostate PrimaCell: Normal Prostate Epithelial Cells
2-82580	CHI Scientific	1 Kit	Ask for price

Rat Ureter PrimaCell: Normal Ureter Epithelial Cells
2-82588	CHI Scientific	1 Kit	Ask for price

Rat Urethral PrimaCell: Normal Urethral Epithelial Cells
2-82589	CHI Scientific	1 Kit	Ask for price

Rat Pancreas PrimaCell 2: Pancreatic Epithelial Cells
2-82597	CHI Scientific	1 Kit	Ask for price

Immortalized Baby Mouse Kidney Epithelial Cells (iBMK)
T0082	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Mouse Pancreas Epithelial Cells-Conditionally (IMPE)
T0156	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Rat Conjunctival Epithelial Cells (CJ4.1A) - SV40
T0368	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Rat Conjunctival Epithelial Cells (CJ4.3) - SV40
T0369	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Mouse Lung Epithelial Cells (MLE-15)
T0495	ABM	1x106 cells / 1.0 ml	EUR 686.4

Immortalized Mouse Colonic Epithelial Cells-Conditionally (YAMC)
T0567	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Mouse Stomach Epithelial Cells-Conditionally (ImSt)
T0569	ABM	1x106 cells / 1.0 ml	Ask for price

Mouse Anti Human Fibroblasts/Epithelial Cells Monoclonal Antibody,RPE
DMABT-49595MH	Creative Diagnostics	25 TEST	EUR 514.8

Human Airway PrimaCell1: Normal Airway Epithelial Cells Growth Medium
9-46001	CHI Scientific	5 x 100 ml	Ask for price

Human Bladder PrimaCell1: Normal Bladder Epithelial Cells Growth Medium
9-46007	CHI Scientific	5 x 100 ml	Ask for price

Human Cervix PrimaCell1: Normal Cervical Epithelial Cells Growth Medium
9-46044	CHI Scientific	5 x 100 ml	Ask for price

Human Cervix PrimaCell4: Normal Endometrial Epithelial Cells Growth Medium
9-46047	CHI Scientific	5 x 100 ml	Ask for price

Human Esophagus PrimaCell1: Normal Esophageal Epithelial Cells Growth Medium
9-46055	CHI Scientific	5 x 100 ml	Ask for price

Human Eye PrimaCell2: Normal Corneal Epithelial Cells Growth Medium
9-46058	CHI Scientific	5 x 100 ml	Ask for price

Human Eye PrimaCell4: Normal Lens Epithelial Cells Growth Medium
9-46060	CHI Scientific	5 x 100 ml	Ask for price

Human Kidney PrimaCell2: Normal Kidney Epithelial Cells Growth Medium
9-46075	CHI Scientific	5 x 100 ml	Ask for price

Human Ovary PrimaCell1: Normal Ovarian Epithelial Cells Growth Medium
9-46094	CHI Scientific	5 x 100 ml	Ask for price

Human Prostate PrimaCell: Normal Prostate Epithelial Cells Growth Medium
9-46099	CHI Scientific	5 x 100 ml	Ask for price

This study aims to evaluate the antioxidant effects of oregano extract in the human spermatozoa cryopreserved. In the first phase, 13 cement samples with different oregano extract concentrations (0.0, 50, 100, 150, 300, and 500 μg / mL) were cryopreserved to achieve the optimal dose of oregano extract. Then, the motility, feasibility, and integrity of plasma membranes is evaluated. In the second phase, 20 cryopreserved samples in frozen media were equipped with or without Optimal Oregano concentration (100 μg / ml). After melting, motility, ROS levels, lipid peroxidation, and phosphatid berserin (PS) translocation are evaluated. The results showed that oregano extract 100 μg / mL significantly increased total motility in the freezing spermatzoa compared to