We have previously reported successful insulation and cryopreservation of human intestinal mucosa (CHIM) with the viability retention and activity of drug metabolism enzymes. Here we report the results of quantification of drug metabolism activities in Chim from various regions in the small intestine of 14 individual donors. Chim isolated from the duodenum, jejunum, and ileum of 10 individuals, and from 10 consecutive 12-inch segments ranging from pyloric sfingter from the small human intestine of four additional individuals.

P450 and non-P450 medicine metabolization enzymes (CYP1A2, CYP2C8, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1, CYP3A, UGT, Sust, AO, NAT1, and NAT2) are measured through selective substrate incubation. Activities that can be measured are observed for all lanes except for CYP2A6. Comparison of duodenal, jejunum, and ileum in 10 donors showing Jejunum has a higher activity for CYP2C9, CYP3A, UGT, SULT, MAO, and NAT1. More definitions of regional variations with Chim from ten proximal small intestinal segments show that the segment immediately after the first 12-inch segment (duodenum) has the highest activity for most drug metabolism enzymes but with a substantial difference in four donors. Our overall results show that there are substantial individual differences in drug metabolism enzymes and that Jejunum, especially the region as soon as the duodenum, has the highest drug metabolic enzyme activity.

The effects of leukocyte anti-human antigen immunity sensitization from allographic cryopreserved and blood transfusions

Allotransplansis of vascular composite (VCA) is the most sophisticated reconstructive technique available for patients who suffer devastating burns into legs or faces. However, VCA requires matching donors. Burn patients have reportedly experienced sensitization, or the development of anti-human antigen antigens leukocyte, during resuscitation and wound coverage, potentially blocking them from future VCA. This study strives to investigate blood and allographical contributions to sensitization in burn patients. Four groups compared: burns of burns that accept blood products and allograft (group 1), burn patients who only accept blood products (group 2), trauma patients only accept blood products (group 3), and healthy volunteer control (Group 4). The average calculation of antibodies of the reactive panel (shows sensitization) is higher in group 1 compared to group 4 (p = 0.035).

In addition, the incidence of severe severe sensitization in group 1 was relative to other groups (p = 0.049). When comparing groups of patients who do not have sensitization, minor sensitization, moderate sensitization, and severe sensitization, there is no significant difference in age, gender, blood products received, the total body surface area burned, or allograft used between groups, though Very sensitive patients tend to have the involvement of a larger body surface area and receive more packaged red blood cell units and allograft (p = 0.079, p = 0.196, and p = 0.072, respectively). Therefore we conclude that while burning patients who receive allograft and blood show a higher incidence of the sensitization of leukocyte anti-human antigens relative to healthy control, this difference cannot be only associated with the use of allograft or transfusions.

Application of Cryopreserved Human Intestinal Mucosa and Human Enterocyt CryoPreserved in the Evaluation of Herb-Drug Interaction: Evaluation of the Potential inhibition of CYP3A Juice Balinese Juice and Twenty-Nine Commercial Formulation Herbal Supplements

Commercial Formulations 29 Herbal supplements commonly used (HS) and grapefruit juice are evaluated for potential drug interactions through the quantification of their CYP3A inhibition potential in two experimental models in vitro from the small intestine of humans, cryopreserved human intestinal mucosa and the Human Enterocyte CryoPreserved (CHE). Two CYP3A substrates are used – in studies with Chim, CYP3A activity is quantified through the quantification of LC / MS MSZolam 1′-hydroxylation while in CHE, Luciferin Science metabolism to Luciferin is quantified by luminescence.

After Chim’s treatment with the estimated concentration of HS lumen in every oral administration (the suggested dose was dissolved in 200 ml of culture media),> 80% inhibition of CYP3A was observed for green tea extract, Valeria roots, St. John, horehound juice and grapefruit. Obstacles of less than 50% observed for fenugreek, aloe vera, guarana, soybean isoflavone, maca, echinacea, spirulina, evening primrose, milk thistle, cranberry, ria yeast, rhodiola, ginkgo biloba, turmeric, curcumin, white beans, garlic, garlic, Cinnamon, see palmetto berry fruit, panax ginseng, black grass juice, wheat grass juice, flaxseed oil, black cohosh, and ginger root. The results are confirmed in the study of dose-responses with HS obtained from three suppliers for four HS inhibition (green tea extract, horehound, St. John’s Wort, Root Valerian), and three HS Black Elderberry (Black Cohosh, Black Elderberry), Echinacea).

Similar results were obtained with HS inhibitors in Che. The results illustrate that Chim and Che represent an experimental model in vitro which is physiologically relevant to evaluate the potential for herbal supplements drug interactions. Significance Statement in Evaluation in Vitro 29 Popular Herbal Supplements in Cryopreserved Human Intestinal Mucosa Identifying Green Tea Extract, Horehound, St. John’s Wort, Root Valerian to have the potential for inhibition of CYP3A similar to grapefruit juice, indicates their potential to have a clinically significant pharmacokinetics. Interaction with orally managed drugs is the CYP3A substrate. The results showed that Chim could be used for evaluating in vitro drug interactions involving enteric drug metabolism.

Extract Vulgare Origanum Aqueous improves the quality of human spermatozoa cryopreserved through its antioxidant effects

Excessive production of reactive oxygen species (ROS) during cement cryopreservation can cause structural and functional changes in spermatozoa. It is known that antioxidants can reduce the ROS effect. In addition, the application of antioxidants in media freezing is the right strategy to protect spermatozoa to the adverse effects of ROS during the cryopreservation process. For example, Oregano is a medicinal plant with important activities, with antiseptic properties, antibacterial, antitrombotic, and antioxidants.

Human Bronchial Epithelial Cells
ALHE47	Alphabioregen	1ml frozen Vial	EUR 620

Human Primary Pulmonary Fibrosis Bronchial Epithelial Cells
T5462	ABM	1 x 10^6 / 1.0 ml	Ask for price

Human Lobar Bronchial Epithelial Cells
ABC-TC3881	AcceGen	1 vial	Ask for price
Description: Special edition cells are isolated from the tissue types described. Cells are sterility and virus tested. All tissues used for cell isolation are obtained under informed consent. Gentaur complies with the U.S. Health Insurance Portability and Accountability Act (HIPAA). Characterization standards for special edition cells have not been established.

Human Bronchial Epithelial Cells with RA
ABC-TC3139	AcceGen	1 vial	Ask for price
Description: Normal Human Bronchial /Tracheal Epithelial Cells (NHBE) are available with or without retinoic acid. These cells are isolated from normal donor airway epithelial tissue located above the bifurcation of the lungs.

Human Primary ∆F508/∆1152H Cystic Fibrosis Bronchial Epithelial Cells
T5425	ABM	5 x 10^5 / 1.0 ml	Ask for price

Rat Bronchial Epithelial Cells
ABC-TC4284	AcceGen	1 vial	Ask for price
Description: Rat Primary Bronchial Epithelial Cells from Gentaur are isolated from bronchial tissue of 6-8 week old laboratory Sprague–Dawley rat. Rat Primary Bronchial Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur’ Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 0.5×10^6 cells and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.

Human Bronchial Epithelial Cells without RA
ABC-TC3140	AcceGen	1 vial	Ask for price
Description: Normal Human Bronchial /Tracheal Epithelial Cells (NHBE) are available with or without retinoic acid. These cells are isolated from normal donor airway epithelial tissue located above the bifurcation of the lungs.

Human Primary DF508/DF508 Cystic Fibrosis Bronchial Epithelial Cells
T5423	ABM	5 x 10^5 / 1.0 ml	Ask for price

Human Primary DF508/N1303K Cystic Fibrosis Bronchial Epithelial Cells
T5424	ABM	5 x 10^5 / 1.0 ml	Ask for price

Human Primary DF508/S549N Cystic Fibrosis Bronchial Epithelial Cells
T5427	ABM	1 x 10^6 / 1.0 ml	Ask for price

Human Bronchial/Tracheal Epithelial Cells
ABC-TC3448	AcceGen	1 vial	Ask for price
Description: Human Bronchial/Tracheal Epithelial Cells, when grown in LIBro Medium, provide an ideal serum-free culture model for the accurate studies of toxicity, cystic fibrosis, asthma, pathogenesis, pharmacology or airway wound healing. HBTEC are cultured without retinoic acid and cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our HBTEC are quality tested in LIBro B/T Medium to ensure optimal growth over a period of at least 15 population doublings at rates equal to or greater than other commercially available media. Cell Features:HBTEC are cryopreserved as primary cells. Cells are isolated from human bronchi/trachea and expanded once in culture vessels before being harvested for cryopreservation.HSAEC are cryopreserved after primary cells are isolated from human lung tissue and expanded once in culture vessels before being harvested for cryopreservation. Human Airway Epithelial Cells are not exposed to retinoic acid during isolation or expansion.Human Airway Epithelial Cells are extensively tested for quality and optimal performance.Gentaur guarantees performance and quality.

Human Bronchial Epithelial Cells for B-ALI
ABC-TC3138	AcceGen	1 vial	Ask for price
Description: Normal Human Bronchial /Tracheal Epithelial Cells (NHBE) are available with or without retinoic acid. These cells are isolated from normal donor airway epithelial tissue located above the bifurcation of the lungs.

Human Pulmonary Lobar Bronchial Epithelial Cells
cAP-0123	Angio Proteomie	1Frozen Vial	EUR 594

Immortalized Human Bronchial Epithelial Cells (HBE1)
T0680	ABM	1x106 cells / 1.0 ml	EUR 686.4

Immortalized Human Bronchial Epithelial Cells - SV40
T0486	ABM	1x106 cells / 1.0 ml	EUR 490

Human Primary DF508/621+1G>T Cystic Fibrosis Bronchial Epithelial Cells
T5426	ABM	5 x 10^5 / 1.0 ml	Ask for price

Immortalized Human Bronchial Epithelial Cells-hTERT
T0496	ABM	1x106 cells / 1.0 ml	EUR 1243.2

C57BL/6 Mouse Primary Tracheal and Bronchial Epithelial Cells
ABC-TC4285	AcceGen	1 vial	Ask for price
Description: C57BL/6 Mouse Primary Tracheal and Bronchial Epithelial Cells from Gentaur are isolated from tracheal and bronchial tissue of pathogen-free laboratory mice. C57BL/6 Mouse Primary Tracheal and Bronchial Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur’ Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5×106 cells per ml and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM-1 (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.

Immortalized Human Bronchial Epithelial Cells-hTERT/BMI
T0498	ABM	1x106 cells / 1.0 ml	EUR 490

Immortalized Human Bronchial Epithelial Cells-hTERT+CDK4
CSC-I9214L	Creative Bioarray	One Frozen vial	Ask for price

Immortalized Human Bronchial Epithelial Cells-hTERT+CDK4
T0497	ABM	1x106 cells / 1.0 ml	EUR 490

Human Pulmonary Bronchial/Tracheal Epithelial Cells
cAP-0124	Angio Proteomie	1Frozen Vial	EUR 594

Human Primary Idiopathic Pulmonary Arterial Hypertension (IPAH) Bronchial Epithelial Cells
T5472	ABM	5 x 10^5 / 1.0 ml	Ask for price

Human Airway PrimaCell™2: Normal Bronchial and Tracheal Epithelial Cells
2-96002	CHI Scientific	1 Kit	EUR 1132.11

DHBE-As (Diseased Human Bronchial Epithelial Cells - Asthma)
LO194911	Westburg	each	EUR 2405.7

DHBE-COPD (Diseased Human Bronchial Epithelial Cells - COPD)
LO195275	Westburg	each	EUR 2405.7

ACE2-GFP Human Pulmonary Lobar Bronchial Epithelial Cells
HPEC001	Neuromics	each	EUR 1165.32

Immortalized Mouse Bronchial Epithelial Cells-SV40
CSC-I2176Z	Creative Bioarray	One Frozen vial	Ask for price

DHBE-CF (Diseased Human Bronchial Epithelial Cells - Cystic Fibrosis)
LO196979	Westburg	each	EUR 2914.41

Immortalized Human Bronchial Epithelial Cells (HBEC3-KT) - Cdk4/hTERT
T0753	ABM	1x10^6 cells / 1.0 ml	EUR 490

NHBE (Normal Human Bronchial Epithelial Cells) w/ Retinoic Acid, BEGM
LOCC-2540	Westburg	each	EUR 1861.75

Rat Airway PrimaCell™1: Normal Bronchial and Tracheal Epithelial Cells
2-82501	CHI Scientific	1 Kit	EUR 943.11

ACE2-GFP Human Pulmonary Bronchial/Tracheal Epithelial Cells
HPEC003	Neuromics	each	EUR 1165.32

ACE2-GFP Expressing Human Pulmonary Lobar Bronchial Epithelial Cells
cAP-0123ACE2-GFP	Angio Proteomie	1Frozen Vial	EUR 841.5

Human Airway PrimaCell™2: Normal Bronchial and Tracheal Epithelial Cells Growth Medium
9-46002	CHI Scientific	5 x 100 ml	EUR 151.2

Mouse Airway PrimaCell™1: Normal Bronchial and Tracheal Epithelial Cells
2-82001	CHI Scientific	1 Kit	EUR 943.11

16HBE14o- Human Bronchial Epithelial Cell Line
ABI-TC4172	AcceGen	1 vial	Ask for price
Description: 16HBE14o- is a human bronchial epithelial cell line isolated from a 1-year old male heart-lung patient and immortalized with the origin-of-replication defective SV40 plasmid (pSVori-). The cell line retains characteristic features of normal differentiated bronchial epithelial cells including a cobblestone morphology, cytokeratin expression, the ability to form tight junctions, and directional ion transport. When grown with an air/liquid interface, cilia can be detected. In contrast to most other respiratory cell lines, 16HBE14o- expresses high levels of cystic fibrosis transmembrane conductance regulator (CFTR) mRNA and protein. Expression of CFTR is correlated to cAMP-dependent Cl- conductance in a variety of cells, including 16HE14o- epithelial cells.

ACE2-GFP Expressing Human Pulmonary Bronchial/Tracheal Epithelial Cells
cAP-0124ACE2-GFP	Angio Proteomie	1Frozen Vial	EUR 841.5

Rat Airway PrimaCell™1: Normal Bronchial and Tracheal Epithelial Cells Growth Medium
9-25001	CHI Scientific	5 x 100 ml	EUR 122.85

Mouse Airway PrimaCell™1: Normal Bronchial and Tracheal Epithelial Cells Growth Medium
9-32001	CHI Scientific	5 x 100 ml	EUR 122.85

Immortalized Human Bronchial Epithelial Cell Line-hTERT+CDK4
ABI-TC065D	AcceGen	1 vial	Ask for price
Description: Immortalised Human Bronchial Epithelial Cells are transducted with recombinant lentiviruses carrying hTERT and CDK4 genes

Bronchial Epithelial Medium Kit
TM044	ABM	1 Kit	EUR 385

Human Airway Tissue Preparation Buffer 2: Normal Bronchial and Tracheal Epithelial Cells
9-80002	CHI Scientific	1 x 100 ml	EUR 160.65

Human Airway PrimaCell™2: Normal Bronchial and Tracheal Epithelial Cells Growth Supplements with Serum (for 500 ml medium)
9-47002	CHI Scientific	1 Set	EUR 321.3

BEGM Bronchial epithelial SingleQuot Kit
LOCC-4175	Westburg	each	EUR 222.93

Rat Airway Tissue Preparation Buffer 1: Normal Bronchial and Tracheal Epithelial Cells
9-80208	CHI Scientific	1 x 100 ml	EUR 132.3

Rat Airway PrimaCell™1: Normal Bronchial and Tracheal Epithelial Cells Growth Supplements with Serum (for 500 ml medium)
9-26001	CHI Scientific	1 Set	EUR 283.5

Mouse Airway PrimaCell™1: Normal Bronchial and Tracheal Epithelial Cells Growth Supplements with Serum (for 500 ml medium)
9-33001	CHI Scientific	1 Set	EUR 283.5

Mouse Airway Tissue Preparation Buffer 1: Normal Bronchial and Tracheal Epithelial Cells
9-80115	CHI Scientific	1 x 100 ml	EUR 160.65

BEBM Bronchial Epithelial Basal Medium, 500 ml
LOCC-3171	Westburg	each	EUR 248.37

BEGM Bronchial epithelial BulletKit (CC-3171+CC-4175)
LOCC-3170	Westburg	each	EUR 389.7

Normal Human Primary Broncial Epithelial Cells
T5511	ABM	5x10^5 cells / 1.0 ml	EUR 875

Human Primary Bronchial Smooth Muscle Cells
T4012	ABM	5x10^5 cells / 1.0 ml	EUR 975

Human Bronchial Smooth Muscle Cells
ABC-TC3530	AcceGen	1 vial	Ask for price
Description: Human Bronchial Smooth Muscle Cells from Gentaur Research Laboratories are isolated from human bronchi and bronchioles. HBSMC are cryopreserved at secondary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HBSMC are characterized by immunofluorescent method with antibodies to smooth muscle actin and desmin. HBSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBSMC are guaranteed to further expand for 15 population doublings at the condition provided by Gentaur Research Laboratories.

Human Bronchial Smooth Muscle Cells
ALHE48	Alphabioregen	1ml frozen Vial	EUR 620

Human Primary Renal Epithelial Cells
T4090	ABM	5x10^5 cells / 1.0 ml	Ask for price

Human Primary Nasal Epithelial Cells
T4014	ABM	5x10^5 cells / 1.0 ml	EUR 1375

Human Primary Thymic Epithelial Cells
T4980	ABM	5x10^5 cells / 1.0 ml	EUR 975

Human Primary Mammary Epithelial Cells
T4036	ABM	5x10^5 cells / 1.0 ml	EUR 625

Human Primary Bladder Epithelial Cells
T4161	ABM	5x10^5 cells / 1.0 ml	Ask for price

Human Primary Corneal Epithelial Cells
T4058	ABM	5x10^5 cells / 1.0 ml	EUR 1475

Primary Human Prostate Epithelial Cells
CSC-C9039J	Creative Bioarray	One Frozen vial	Ask for price

Human Primary Prostate Epithelial Cells
T4078	ABM	5x10^5 cells / 1.0 ml	EUR 625

Human Primary Amniotic Epithelial Cells
T4109	ABM	5x10^5 cells / 1.0 ml	EUR 625

Human Primary Tracheal Epithelial Cells
T4007	ABM	5x10^5 cells / 1.0 ml	EUR 625

Human Primary Placental Epithelial Cells
T4076	ABM	5x10^5 cells / 1.0 ml	EUR 625

Human Primary Esophageal Epithelial Cells
T4230	ABM	5x10^5 cells / 1.0 ml	EUR 1375

Human Primary Small Airway Epithelial Cells
T4008	ABM	5x10^5 cells / 1.0 ml	EUR 625

Human Airway PrimaCell™3: Normal Bronchial and Tracheal Smooth Muscle Cells
2-96003	CHI Scientific	1 Kit	EUR 1228.5

Human Primary Mammary Epithelial Cells (HMEpC)
P0006038	Addexbio	One Frozen vial	EUR 600

Immortalized Human Bronchial Smooth Muscle Cells
T0493	ABM	1x106 cells / 1.0 ml	EUR 2750

Human Primary Renal Cortical Epithelial Cells
T4088	ABM	5x10^5 cells / 1.0 ml	EUR 975

Human Primary Colonic Epithelial cells (HCoEpC)
P0009028	Addexbio	One Frozen vial	EUR 650

Normal Human Primary Tracheal Epithelial Cells
T5512	ABM	5x10^5 cells / 1.0 ml	EUR 875

Normal Human Primary Tracheal Epithelial Cells
T5513	ABM	5x10^5 cells / 1.0 ml	EUR 875

Human Primary Cervical Epithelial Cells (HCxEpC)
P0008008	Addexbio	One Frozen vial	EUR 650

Human Primary Prostate Epithelial Cells (HPrEpC)
P0019010	Addexbio	One Frozen vial	EUR 700

Human Primary Ovarian Surface Epithelial Cells
T4198	ABM	5x10^5 cells / 1.0 ml	EUR 975

Human Primary Retinal Pigment Epithelial Cells
T4057	ABM	5x10^5 cells / 1.0 ml	EUR 1375

Human Primary Alveolar Epithelial Cells - Type II
T4116	ABM	5x10^5 cells / 1.0 ml	EUR 3695

Normal Human Primary Small Airway Epithelial Cells
T5515	ABM	5x10^5 cells / 1.0 ml	EUR 875

Human Thyroid PrimaCell™: Thyroid Epithelial Cells
2-96124	CHI Scientific	1 Kit	EUR 1132.11

Normal Human Primary Intestinal Epithelial Cells Ilium
T5528	ABM	5x10^5 cells / 1.0 ml	EUR 875

Human Primary Intra-Hepatic Biliary Epithelial Cells
T4221	ABM	5x10^5 cells / 1.0 ml	Ask for price

Normal Human Primary Intestinal Epithelial Cells Jejunum
T5527	ABM	5x10^5 cells / 1.0 ml	EUR 875

Rat Primary Lung Epithelial Cells
T4800	ABM	5x10^5 cells / 1.0 ml	EUR 625

Normal Human Primary Intestinal Epithelial Cells Duodenem
T5526	ABM	5x10^5 cells / 1.0 ml	EUR 875

Human Airway PrimaCell™3: Normal Bronchial and Tracheal Smooth Muscle Cells Growth Medium
9-46003	CHI Scientific	5 x 100 ml	EUR 151.2

Human Eye PrimaCell™4: Normal Lens Epithelial Cells
2-96060	CHI Scientific	1 Kit	EUR 1132.11

Rat Primary Renal Epithelial Cells
T4936	ABM	5x10^5 cells / 1.0 ml	Ask for price

Human Lung PrimaCell™ 7: Alveolar Epithelial Cells II
2-96122	CHI Scientific	1 Kit	EUR 1132.11

Human Pancreas PrimaCell™ 2: Pancreatic Epithelial Cells
2-96123	CHI Scientific	1 Kit	EUR 1132.11

Rat Primary Bladder Epithelial Cells
T4940	ABM	5x10^5 cells / 1.0 ml	Ask for price

Human Eye PrimaCell™2: Normal Corneal Epithelial Cells
2-96058	CHI Scientific	1 Kit	EUR 1132.11

Human Colon PrimaCell™: Normal Colorectal Epithelial Cells
2-96115	CHI Scientific	1 Kit	EUR 1132.11

Rat Primary Tracheal Epithelial Cells
T4804	ABM	5x10^5 cells / 1.0 ml	Ask for price

Rat Primary Cervical Epithelial Cells
T4952	ABM	5x10^5 cells / 1.0 ml	Ask for price

Rat Primary Prostate Epithelial Cells
T4956	ABM	5x10^5 cells / 1.0 ml	Ask for price

Human Prostate PrimaCell™: Normal Prostate Epithelial Cells
2-96099	CHI Scientific	1 Kit	EUR 1132.11

Human Urethral PrimaCell™: Normal Urethral Epithelial Cells
2-96113	CHI Scientific	1 Kit	EUR 1132.11

Mouse Primary Corneal Epithelial Cells
T4571	ABM	5x10^5 cells / 1.0 ml	EUR 1375

Mouse Primary Bladder Epithelial Cells
T4640	ABM	5x10^5 cells / 1.0 ml	EUR 975

Human Airway PrimaCell™1: Normal Airway Epithelial Cells
2-96001	CHI Scientific	1 Kit	EUR 1132.11

Human Kidney PrimaCell™2: Normal Kidney Epithelial Cells
2-96075	CHI Scientific	1 Kit	EUR 1132.11

Human Ovary PrimaCell™1: Normal Ovarian Epithelial Cells
2-96094	CHI Scientific	1 Kit	EUR 1228.5

Human Ureter PrimaCell™2: Normal Ureter Epithelial Cells
2-96112	CHI Scientific	1 Kit	EUR 1132.11

Rat Primary Esophageal Epithelial Cells
T4912	ABM	5x10^5 cells / 1.0 ml	Ask for price

Mouse Primary Tracheal Epithelial Cells
T4502	ABM	5x10^5 cells / 1.0 ml	Ask for price

Mouse Primary Cervical Epithelial Cells
T4650	ABM	5x10^5 cells / 1.0 ml	Ask for price

Mouse Primary Prostate Epithelial Cells
T4654	ABM	5x10^5 cells / 1.0 ml	Ask for price

Rat Airway PrimaCell™2: Normal Bronchial and Tracheal Smooth Muscle Cells
2-82502	CHI Scientific	1 Kit	EUR 1039.5

Human Bladder PrimaCell™1: Normal Bladder Epithelial Cells
2-96007	CHI Scientific	1 Kit	EUR 1228.5

Human Cervix PrimaCell™1: Normal Cervical Epithelial Cells
2-96044	CHI Scientific	1 Kit	EUR 1132.11

Normal Human Primary Intestinal Epithelial Cells Ascending Colon
T5523	ABM	5x10^5 cells / 1.0 ml	EUR 975

Mouse Primary Esophageal Epithelial Cells
T4612	ABM	5x10^5 cells / 1.0 ml	Ask for price

Mouse Airway PrimaCell™2: Normal Bronchial and Tracheal Smooth Muscle Cells
2-82002	CHI Scientific	1 Kit	EUR 1039.5

Normal Human Primary Intestinal Epithelial Cells Transverse Colon
T5524	ABM	5x10^5 cells / 1.0 ml	EUR 975

Normal Human Primary Intestinal Epithelial Cells Descending Colon
T5525	ABM	5x10^5 cells / 1.0 ml	EUR 975

Human Cervix PrimaCell™4: Normal Endometrial Epithelial Cells
2-96047	CHI Scientific	1 Kit	EUR 1228.5

Human Stomach PrimaCell™: Normal Stomach Mucosa Epithelial Cells
2-96104	CHI Scientific	1 Kit	EUR 1132.11

Primary Rabbit Thymic Epithelial Cells (TEC)
CSI116Rb01	Cloud-Clone	5X10^5	EUR 1160

Human Breast PrimaCell™: Normal Mammary Epithelial Primary Cells
2-96023	CHI Scientific	1 Kit	EUR 1228.5

This study aims to evaluate the antioxidant effects of oregano extract in the human spermatozoa cryopreserved. In the first phase, 13 cement samples with different oregano extract concentrations (0.0, 50, 100, 150, 300, and 500 μg / mL) were cryopreserved to achieve the optimal dose of oregano extract. Then, the motility, feasibility, and integrity of plasma membranes is evaluated. In the second phase, 20 cryopreserved samples in frozen media were equipped with or without Optimal Oregano concentration (100 μg / ml). After melting, motility, ROS levels, lipid peroxidation, and phosphatid berserin (PS) translocation are evaluated. The results showed that oregano extract 100 μg / mL significantly increased total motility in the freezing spermatzoa compared to