Lnc RNA ZFAS1 regulates the proliferation, apoptosis, inflammatory response and autophagy of fibroblast-like synoviocytes via miR-2682-5p/ADAMTS9 axis in Rheumatoid Arthritis
Background: Rheumatoid arthritis (RA) is an autoimmune disease often. Emerging evidence suggests that antisense ZNFX1 RNA1 (ZFAS1) participate in physiological and pathological processes in RA. However, knowledge ZFAS1 in RA is limited, the potential paths ZFAS1 work needs to be investigated further.
Methods: Levels ZFAS1, microRNA (mir) -2682-5p, and ADAM metallopeptidase with thrombospondin type 1 motif 9 (ADAMTS9) were estimated using real-time quantitative polymerase chain reaction (qRT-PCR) assay. 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay was performed to explore the capabilities of cell proliferation in fibroblast-like synoviocytes (FLS-RA). Cell apoptosis was measured by flow cytometry. Also, the rate ADAMTS9, a protein associated with apoptosis, Cleaved-caspase-3 (active large subunit), and autophagy-related protein identified adopt western blot. Enzyme-linked immunosorbent assay (ELISA) was conducted to determine the production of inflammatory cytokines. In addition, the relationship between miR-2682-5p and ZFAS1 or ADAMTS9 verified utilize dual-luciferase reporter assay.
Results: High levels of ZFAS1 and ADAMTS9, and low levels of miR-2682-5p were observed in RA synovial tissue and FLS-RA. Knockdown of ZFAS1 cause a reduction of cell proliferation, inflammation, autophagy, and increased apoptosis in RA-FLS, while this effect was abolished by getting back in the inhibition of miR-2682-5p. In addition, miR-2682-5p effect on the phenotype of cells and the inflammatory response was eliminated by ADAMTS9 upregulation in RA-FLS. Mechanically, ZFAS1 given its role through miR-2682-5p / ADAMTS9 axis in RA.
Conclusion: ZFAS1 / miR-2682-5p / ADAMTS9 axis can modulate the behavior of cells, the inflammatory response in RA-FLS, may provide potential therapeutic targets for the treatment of RA. Keywords: Rheumatoid arthritis; cell autophagy; inflammatory.
Lnc RNA ZFAS1 regulates the proliferation, apoptosis, inflammatory response and autophagy of fibroblast-like synoviocytes via miR-2682-5p/ADAMTS9 axis in Rheumatoid Arthritis
Estrogen downregulates the expression of TAK1 in human fibroblast-like synoviocytes and models of rheumatoid arthritis
Transforming growth factor-β-activated kinase 1 (TAK1), a member of the protein kinase family of mitogen-activated, plays a key role in the pathogenesis and progression of rheumatoid arthritis (RA). Estrogen has been reported previously to delay the progression of arthritis. However, the exact association between TAK1 and estrogen remains elusive. This study demonstrated that TAK1 synoviocytes is upregulated in patients with RA compared to patients with osteoarthritis and healthy controls. In addition, the TAK1 also expressed in cultured fibroblast-like synoviocytes (FLS), and the levels were decreased significantly in 17β-estradiol (E2) -treated cells in a dose-dependent manner
. In addition, administration of E2 significantly decreased expression of TAK1 and attenuated the development of collagen-induced arthritis (CIA). Taken together, the findings from this study suggest that E2 mediates TAK1 decline in both FLS and the CIA, which later resulted in the suppression of pathological processes CIA. Therefore, estrogen may serve as a potential therapeutic agent for the treatment of RA by targeting TAK1.
Description: Human synoviocytes (HS), the predominant cell type of healthy synovial tissue, are fibroblast-like cells. The Synoviocytes form a distinct structure called the synovial lining layer. Electron microscopy revealed extensive cell-to-cell contacts within the lining layer. The Synoviocytes produce synovial fluid components and are responsible for absorption from the joint cavity, and for blood/synovial fluid exchanges. The synoviocytes proliferate, show anchorage-independent growth, and also secrete a variety of effector molecules that promote inflammation and joint destruction and, themselves are part of a complex network of autocrine and paracrine acting factors. The synoviocytes express cadherin-11 providing new insight into synovial tissue organization and morphogenesis and of interest as a therapeutic target.HS from Gentaur Research Laboratories are isolated from human synovium. HS are cryopreserved at passage one cultures and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HS are characterized by their fibroblast-like morphology, growth pattern and immunocytochemistry of CD 90 and fibronectin. HS are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HS are guaranteed for 15 population doublings at the conditions provided by Gentaur Research Laboratories.
Description: Human Fibroblast-Like Synoviocytes (HFLS) are isolated from normal synovial tissues. They are cryopreserved at second passage and can be cultured and propagated at least 5 population doublings.
Description: Human Fibroblast-Like Synoviocytes (HFLS) are isolated from synovial tissues obtained from patients with Rheumatoid Arthritis (RA) / Osteoarthritis (OA). They are cryopreserved at second passage and can be cultured and propagated at least 5 population doubling.
Description: Human Fibroblast-Like Synoviocytes (HFLS) are isolated from synovial tissues obtained from patients with Rheumatoid Arthritis (RA) / Osteoarthritis (OA). They are cryopreserved at second passage and can be cultured and propagated at least 5 population doubling.
COOLCELL SV2 STEMCELL CRYOPRESERVATION SYSTEM INCLUDES THE XT STARTER BENCHTOP COOLER, COOLRACK SV2 AND COOLCELL SV2 CELL FREEZING CONTAINER FOR 12 INJECTABLE VIALS
COOLCELL SV10 STEMCELL CRYOPRESERVATION SYSTEM INCLUDES THE XT STARTER BENCHTOP COOLER, COOLRACK SV10 AND COOLCELL SV10 CELL FREEZING CONTAINER FOR 6 INJECTABLE VIALS
Description: Quantitative sandwich ELISA for measuring Human E3 ubiquitin-protein ligase synoviolin (SYVN1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Recombinant Human E3 ubiquitin-protein ligase synoviolin (SYVN1)
Since this article has been alleged infringement and corresponding study author did not respond to our request to prove the authenticity of the data and figures, “Mir-20a Regulates fibroblast-like synoviocyte proliferation and apoptosis in rheumatoid arthritis, by X.-J. Wei, X.- W. Li, J.-L. Lu, long Z.-X., J.-Q. Liang, S.-B. Wei, C.-X. Lu, Lu W.-Z., published in Eur Rev Med Pharmacol Sci 2017; 21 (17): 3886-3893-PMID: 28,975,975 “has been withdrawn. Publisher apologizes for any inconvenience this may cause.
