The expression of viral vectors in HEK 293 cells is a widely used procedure to obtain sufficient quantities of the desired virus. The manufacturing and purification process of these products leaves the potential for host cell protein (HCP) impurities from HEK 293 cells. HCP impurities can result in toxic or immunological adverse reactions and are therefore desirable to reduce these impurities to the lowest practical levels.

The polyclonal antibodies used in this kit have been generated and affinity purified using a soft lysate of HEK 293 cells to obtain HCPs which are typically in the initial stages of product recovery. This kit can be used as a process development tool to monitor optimal removal of host cell impurities, as well as in routine end-product release testing when comprehensively characterized using samples from your process.

Antibody affinity extraction (AAE) was used to determine reactivity to individual HCPs in a smooth HEK 293 lysate. AAE indicated reactivity at ~ 74% of individual 2-D PAGE fractionated spots presumed to be HCPs. This HEK 293 HCP ELISA Kit, 3G, cat. No. F650S replaces the previous F650R kit and incorporates several performance enhancements. The F650S kit uses a new, broadly reactive antibody that outperforms the previous F650R antibody.

In addition, the F650S kit has higher sensitivity (LOD 0.2 ng/ml; LOQ 1 ng/ml), a shorter detection and sample / standard Ab incubation time (1.5 h), and greater stability. Use the new Sample Diluent, cat. No. I700 with this kit.

Product Information

  • Product Type: ELISA kit
  • Storage: Standards -10 ° C to -30 ° C upon receipt, All other reagents in the kit 2 ° C to 8 ° C
  • Target expression system: HEK 293
  • Species group: Mammals
  • Human species
  • Kit components
  • Stop solution
  • TBS Wash Concentrate, 20X
  • TMB substrate
  • Anti-HEK 293: HRP Conjugate
  • HEK 293 HCP (A-F) Standards
  • Anti-HEK 293 Coated Microtiter Strips

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