Improvement of in situ Follicular Activation and Early Development in Cryopreserved Human Ovarian Cortical Tissue by Co-Culturing with Mesenchymal Stem Cells.

Improvement of in situ Follicular Activation and Early Development in Cryopreserved Human Ovarian Cortical Tissue by Co-Culturing with Mesenchymal Stem Cells.

Follicle losses and network degeneration are major challenges in the ovarian tissue culture system. Mesenchymal (MSC) stem cells emit the cocktail of growth and cytokines that support adjacent cells and networks. The purpose of this study was to investigate the impact of the human bone marrow (HBM) -MSC, as culture cells, in the development of follicular humans in ovarian cortical tissue (October). For this purpose, the OCT fraction was properly cultured along with HBM-MSC for 8 days and compared to October monoculture. During the cultural period, survival and development of ovarians in October was evaluated using histological observation, gene expression associated with follicular development, and estradiol production.

Furthermore, cell proliferation and apoptosis are assessed. The results showed that there was no significant difference in ovarian follicles which were preserved with normal morphology between the two groups. However, the percentage of follicular development, as well as the expression of follicular development gene, increased significantly in cultural groups compared to monoculture groups. On the other hand, compared to the monoculture group, the culture group showed a significant increase in cell proliferation, indicated by the expression of KI67 gene, and a dramatic decline in the percentage of apoptotic cells, revealed by the Tunel test. These findings indicate that the HBM-MSC co-cultivation with October can increase the activation of follicles and the development of initial follicles in the human ovarian network culture system.

The increase in the efficacy of Vivo from the clinical class of cryopreserved human hepatocytes in mice with acute liver failure.

Clinical clinical hepatocytes of short-term efficacy has been shown; However, some of the main limitations, mainly due to lack of organs, the lack of quality of insulated cells and low cells after transplantation, must be resolved to increase its efficacy in clinical applications. Cellular stress during isolation causes loss of unexpected cell attachment capabilities, which can be exacerbated by cryopreservation and disbursement. In this work, we focus on the use of good manufacturing practices (GMP) solutions compared to standard cryopreservation media, Wisconsin University media, for the purpose of improving the functional quality of cells and its ability to instill in vivo, with the idea of ​​establishing biobank from human hepatocytes Cryopreserved available for use their clinical.

We evaluate not only cell viability but also certain liver function indicators of functional performance of cells such as attachment efficiency, urogenic capabilities, phase and II enzyme enzyme activities and specific adhesion molecular expressions in vitro. In addition, we also assess and compare the efficacy of in vivo from hepatocytes of human hepatocytes in different media in the animal model of acute liver failure. Humatocytes of Cryopreserved Humans in GMP solutions new offers in vitro functionality better and in vivo compared to those who are cryopreserved in standard media. Overall, the results showed that the GMP solution tested newly maintained better liver function and, most importantly, showed better results in Vivo, which could imply an increase in the long-term efficacy when used in patients.

The genetic diagnosis of β-thalassemia preplantation uses short tandem repetition on cryopreserved human blastocythis.

This study aims to evaluate the application of short tandem repetitions (STRS) for the diagnosis of genetic prequlantation (PGD) β-thalassemia. This is a prospective study that appears at the Pregnant Hospital and Liuzhou Child Health. From May to December 2016, eight couples formed from two β-thalassemia operators underwent a procedure and PGD fertilization (IVF) in vitro. All couples and four family members / couples undergo blood testing. Genome amplification of all trophy cell cells is done. PCR products are used for link analysis of 15 strokus. Of the eight couples, 147 embryos were obtained and 86 blastokistas were formed. DNA from 82 Blastokista was successfully reinforced (95.4% amplification efficiency).

Improvement of in situ Follicular Activation and Early Development in Cryopreserved Human Ovarian Cortical Tissue by Co-Culturing with Mesenchymal Stem Cells.

Eighty Blastokista obtained a definite diagnosis. Among them, 24 blastokistas were diagnosed as normal, 38 blastokistas were diagnosed as heterozygous for β-thalassemia, and 18 homozygous blastokistas or heterozygous compounds. Two patients received a melting embryo and both had clinical pregnancies. These results indicate that in the PGD settings for β-thalassemia, after several displacement amplifications, the reverse dot hybridization combined with strs can be an effective, accurate, and practical clinical strategy to increase β-thalassemia detection in pairs that are at risk of transferring embryo. These results must be validated in a larger cohort.

Is the 7th day of the 7th culture for fertilizing in vitro from the oocyte of cryopreserved / warm man?

Human embryos are usually cultured to the Blastokyst stage on day 5 or 6 after insemination. However, some embryos grew slowly and reached the Blastokyst stage on Day 7. The level of direct birth. Acceptable birthday has been reported after the 7-blastocista day transfer is produced from the fresh oockey in vitro fertilization (IVF). It is not known whether an extended embryonic culture until the 7th day is needed for more ivf ocyte ivf to get a more transfer blastocist. In this study, 455 oocytes of 57 cycles were warmed, inseminated, and the resulting embryo was cultured by day 7 to test the development after the expanded culture.

Human Peripheral Blood CD3+ T Cells, Cryopreserved

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Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved

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Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved

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Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved

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Human CD3+ T Cells (Pan T Cells), Cryopreserved

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Human  Peripheral  Blood  CD14+ Monocytes, Cryopreserved / Untouched

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Positively Selected Normal Peripheral Blood CD19+ B Cells, Cryopreserved

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Human  Peripheral  Blood  CD14+ Monocytes, Cryopreserved / Positive selection

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Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Cryopreserved

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Human  Peripheral  Blood  CD4+  T  Lymphocytes, Cryopreserved

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Human CellExp? HGF, Human Recombinant

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Human CellExp? SCF, Human Recombinant

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Human CellExp? TPO, Human Recombinant

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Human CellExp? TPO, Human Recombinant

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Human CellExp? sCD14, Human Recombinant

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Human CellExp? PCSK9, human recombinant

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Human CellExp? CD80, human recombinant

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Human CellExp? CD80, human recombinant

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Human CellExp? CD47, human recombinant

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Human CellExp? TNFRII, human recombinant

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Human CellExp? SERPIND1, human recombinant

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Human CellExp? VTCN1, human recombinant

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Human CellExp? VTCN1, human recombinant

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Human CellExp? CD23, human recombinant

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Human CellExp? EPHB4, human recombinant

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Human CellExp? EPHB4, human recombinant

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Human CellExp? TFPI, human recombinant

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Human CellExp? CD155, human recombinant

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Human CellExp? VLDLR, human recombinant

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Fifty-one blastokista of the 16 cycles of being used to examine the embryo Aneuploidies Day 7 Blastokista accounted for 15.6% of the total blastokist. The proportion of the top quality of the blastokist is lower on the 7th day than on day 5 or 6. However, there is no difference observed at the level of blastokyst aneuploid between days 5, 6 and 7. Similar clinical pregnancies, ongoing pregnancy and embryonic implantation rates Obtained after the 7th day of the Blastocyst transfer compared to day 5 or 6 blastocyst transfers. These results indicate that the embryo of the oocyte heating cycle must be cultured until 7 days if they do not reach the Blastokyst stage on the 6th day so that the number of blastokistas that can be used can be increased.

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