The level of disbursement of iliaca artery of the human kriopreserved allografts (CHIAA) directly affects the fatigue of structural changes that occur during this process.

The level of disbursement of iliaca artery of the human kriopreserved allografts (CHIAA) directly affects the fatigue of structural changes that occur during this process.

Experiments were carried out at ten Chiaa. 10% dimethylsulphoxide in hydroxyethyl 6% starch solution is used as cryoprotectant; All Chiaa is cooled at a level controlled and stored in the liquid nitrogen vapor phase (-194 ° C). Two Disbursement Protocols are tested: (1) placing Chiaa in a water bath at 37 ° C, and (2) Chiaa is disbursed in a controlled environment at 5 ° C. All samples undergo analysis under the scanning electron microscope. Testing of Chiaa’s mechanical properties is evaluated on a single-made strain test engine specifically made. Longitudinal and circular samples were prepared from each Chiaa tested.

Results: Ultrastructoral analysis revealed that the five Chiaa was disbursed during the Disbursement Protocol 1 which showed more damage to the subendotial structure when compared to the disbursed sample in the protocol 2. Mechanical properties: 1-Longitudinal Disbursement Protocol UTS 2, 53 ± 0, 47 MPa on relative strain 1, 27 ± 0, 12 and around UTS 1, 94 ± 0, 27 MPa on the relative strain 1, 33 ± 0, 09. Disbursement Protocol 2-Longitudinal Tensile Strain (UTS) 2, 42 ± 0, 34 MPa on relative strain 1, 32 ± 0, 09 and circular utiat 1, 98 ± 0, 26 MPa on the relative strain of 1, 29 ± 0, 07. Comparing UTS does not show statistical differences between disbursement methods.

Expand the use of test challenges as Biomarkers repair DNA in the human population: protocols for fresh blood samples and cryopreserved, and for different challenges agents

Deficiencies in DNA (DDRR) damage response (DDRR) can cause serious pathological results; Therefore, having the ability to determine individual DDRRs will increase concerns in the assessment of health risks and in determining individual responses to cancer therapy. However, most methods for evaluating DDRR are not fully suitable for population studies. Test challenges have received acceptance for this purpose. The test traditionally uses X-rays as a challenge agent and mononuclear cell peripheral blood isolated (PBMC) as cell specimens. To increase the use of the test, the purpose of this investigation is to use processed blood samples differently, to hire other challenges agents with various induction mechanisms of DNA damage / improvement, and to produce protocols to detect different DDRR capacities.

Fresh and frozen blood samples are challenged by bleomycin, methyl methanesulfonate (MMS) and ultraviolet light. Induction significant damage after all treatments, and progressive DDRR and depends on time observed. There was no significant difference obtained in the capacity of DDRR of blood samples all fresh or frozen compared to PBMC, except that fresh blood samples showed the capacity of DDRR induced by MMS higher than PBMC. The results of this study indicate that the test of comets can be used as routine biomarkers of DDRR capacity in human biomonitor studies, and that all blood is also a biomatrix that is useful for this test. Data collected allows us to recommend various protocols for test-comets that are useful for evaluating DDRR capacity in several main DNA repair lines. As a result, the usefulness of testing the challenges can be greatly expanded.

Micronucleus cytokinesis-block test on fresh blood mononuclear cells and isolated human cryopreserved

The Cytokinesis-Block Micronucleus (CBMN) test is a standard method used for genotoxicity studies. Conventional overall blood culture (WBC) is often used for this test, although this test can also be carried out on isolated blood mononuclear cell culture (PBMC). However, the standardization of the protocol for the CBMN PBMC test has not been widely investigated. The purpose of this study was to optimize the reliable CBMN test protocol for fresh peripheral blood mononuclear cells and cryopreserved (PBMC), and to compare the results of Micronuclei (MNI) between WBC and PBMC culture.

The level of disbursement of iliaca artery of the human kriopreserved allografts (CHIAA) directly affects the fatigue of structural changes that occur during this process.

The CBMN G0 test is carried out in all blood, new isolated, and PBMC cryopreserved from healthy human blood samples and five samples of radiosensitive patients. Cells hit by 220 kv x-ray in vitro ranging from 0.5 to 2 gy. The Optimized PBMC CBMN test shows adequate repetition and small inter-individual variability. MNI value is significantly higher for WBC than for fresh PBMC. In addition, the Cryopreservation of PBMC resulted in an increase in significant MNI values, while different Cryopreservation time did not have a significant impact. In conclusion, our standard CBMN test on fresh PBMC and cryopreserved can be used for genotoxicity, biological dosimmetry, and radiosensitivity assessment.

Bilaminar Engineering with Corriocer Advanced Flap and Cryopreserved Human Amniotic Membranes in the treatment of gingiva recession

Gingiva recession is usually treated with surgical therapy that involves the use of connective network autograft to thicken gingiva network. It has an important biological impact on patients because they will have surgical wounds on two different oral cavity sites. In this case report, we offer an alternative technique for the treatment of Gingiva recession class Miller. A 40-year-old woman patient has been treated with a combination of Bilaminar and Flap Flap technique in a coron to cover the Miller I Gingiva recession with the addition of the cryopreserved human amniotic membrane.

Fibroblasts/Epithelial Cells Antibody

abx413036-25ug 25 ug
EUR 360

Retinal Pigment Epithelial Cells

RPE001 500,000 Cells
EUR 1545.6

Human Thyroid PrimaCell: Thyroid Epithelial Cells

2-96124 1 Kit Ask for price

Human Renal Proximal Tubule Epithelial Cells

SC00A1-PTEC 500,000 Cells
EUR 1585.2

Immortalized Human Pterygium Epithelial Cells - SV40

T0360 1x106 cells / 1.0 ml Ask for price

Immortalized Human Conjuctival Epithelial Cells - SV40

T0361 1x106 cells / 1.0 ml Ask for price

Immortalized Human Nasal Epithelial Cells - SV40

T0440 1x106 cells / 1.0 ml Ask for price

Immortalized Human Bronchial Epithelial Cells - SV40

T0486 1x106 cells / 1.0 ml
EUR 1243.2

Immortalized Human Bronchial Epithelial Cells-hTERT

T0496 1x106 cells / 1.0 ml
EUR 1243.2

Immortalized Human Placental Epithelial Cells - SV40

T0530 1x106 cells / 1.0 ml Ask for price

Immortalized Human Amniotic Epithelial Cells - SV40

T0531 1x106 cells / 1.0 ml Ask for price

Immortalized Human Retinal Pigment Epithelial Cells

T0571 1x106 cells / 1.0 ml
EUR 756

Immortalized Human Lens Epithelial Cells - SV40

T0573 1x106 cells / 1.0 ml
EUR 895.2

Immortalized Human Renal Cortical Epithelial Cells

T0614 1x106 cells / 1.0 ml Ask for price

Immortalized Human Renal Medullary Epithelial Cells

T0615 1x106 cells / 1.0 ml Ask for price

Immortalized Human Nasopharyngeal Epithelial Cells (NP69SV40T)

T0634 1x106 cells / 1.0 ml Ask for price

Immortalized Human Bronchial Epithelial Cells (HBE1)

T0680 1x106 cells / 1.0 ml
EUR 686.4

Immortalized Human Ovarian Epithelial Cells - SV40

T1074 1x106 cells / 1.0 ml Ask for price

Fibroblasts/Epithelial Cells Antibody (RPE)

abx413037-100tests 100 tests
EUR 811.2

Fibroblasts/Epithelial Cells Antibody (RPE)

abx413038-25tests 25 tests
EUR 427.2

Human Airway PrimaCell1: Normal Airway Epithelial Cells

2-96001 1 Kit Ask for price

Human Bladder PrimaCell1: Normal Bladder Epithelial Cells

2-96007 1 Kit Ask for price

Human Cervix PrimaCell1: Normal Cervical Epithelial Cells

2-96044 1 Kit Ask for price

Human Cervix PrimaCell4: Normal Endometrial Epithelial Cells

2-96047 1 Kit Ask for price

Human Esophagus PrimaCell1: Normal Esophageal Epithelial Cells

2-96055 1 Kit Ask for price

Human Eye PrimaCell2: Normal Corneal Epithelial Cells

2-96058 1 Kit Ask for price

Human Eye PrimaCell4: Normal Lens Epithelial Cells

2-96060 1 Kit Ask for price

Human Kidney PrimaCell2: Normal Kidney Epithelial Cells

2-96075 1 Kit Ask for price

Human Ovary PrimaCell1: Normal Ovarian Epithelial Cells

2-96094 1 Kit Ask for price

Human Prostate PrimaCell: Normal Prostate Epithelial Cells

2-96099 1 Kit Ask for price

Human Ureter PrimaCell2: Normal Ureter Epithelial Cells

2-96112 1 Kit Ask for price

Human Urethral PrimaCell: Normal Urethral Epithelial Cells

2-96113 1 Kit Ask for price

Human Colon PrimaCell: Normal Colorectal Epithelial Cells

2-96115 1 Kit Ask for price

Human Pancreas PrimaCell 2: Pancreatic Epithelial Cells

2-96123 1 Kit Ask for price

Immortalized Human Mammary MUC+ESA+ Epithelial Cells

T0453 1x106 cells / 1.0 ml Ask for price

Immortalized Human Mammary Epithelial Cells (HMEC 2.6)

T0454 1x106 cells / 1.0 ml
EUR 1243.2

Immortalized Human Small Airway Epithelial Cells - SV40

T0485 1x106 cells / 1.0 ml
EUR 1243.2

Immortalized Human Bronchial Epithelial Cells-hTERT+CDK4

T0497 1x106 cells / 1.0 ml
EUR 1243.2

Immortalized Human Bronchial Epithelial Cells-hTERT/BMI

T0498 1x106 cells / 1.0 ml
EUR 1243.2

Immortalized Human Esophageal Epithelial Cells (NE2-hTERT)

T0632 1x106 cells / 1.0 ml
EUR 1243.2

Immortalized Human Nasopharyngeal Epithelial Cells (NPEC1/Bmi1)

T0765 1x106 cells / 1.0 ml Ask for price

Immortalized Human Nasopharyngeal Epithelial Cells (NPEC2/Bmi1)

T0766 1x106 cells / 1.0 ml Ask for price

Epithelial Dissociation System 11 (Epithelial Cells), Mouse and Rat

4-20261 ea Ask for price

Mouse Thyroid PrimaCell: Thyroid Epithelial Cells

2-82103 1 Kit Ask for price

Rat Thyroid PrimaCell: Thyroid Epithelial Cells

2-82598 1 Kit Ask for price

Immortalized Mouse Mammary Epithelial Cells (iMMEC)

T0451 1x106 cells / 1.0 ml Ask for price

Immortalized Mouse Prostate Epithelial Cells (iMPEC)

T0607 1x106 cells / 1.0 ml Ask for price

Immortalized Ovarian Surface Epithelial Cells (iMOSEC)

T0608 1x106 cells / 1.0 ml Ask for price

Human Breast PrimaCell: Normal Mammary Epithelial Primary Cells

2-96023 1 Kit Ask for price

Human Eye PrimaCell5: Normal Retinal Pigment Epithelial Cells

2-96061 1 Kit Ask for price

Human Intestine PrimaCell1: Normal Intestinal Mucosa Epithelial Cells

2-96071 1 Kit Ask for price

Human Kidney PrimaCell7: Normal Renal Tubular Epithelial Cells

2-96080 1 Kit Ask for price

Human Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells

2-96104 1 Kit Ask for price

Human Lung PrimaCell 7: Alveolar Epithelial Cells II

2-96122 1 Kit Ask for price

Immortalized Human Mammary Epithelial Cells (HMEC 2.6) - SV40

T0455 1x106 cells / 1.0 ml
EUR 1243.2

Immortalized Human Tonsillar Epithelial Cells (E6/E7 HTE)

T0660 1x106 cells / 1.0 ml Ask for price

Immortalized Human Cystic Fibrosis Tracheobronchial Epithelial Cells (CFT1)

T0681 1x106 cells / 1.0 ml
EUR 686.4

Mouse Bladder PrimaCell1: Normal Bladder Epithelial Cells

2-82006 1 Kit Ask for price

Mouse Cervix PrimaCell1: Normal Cervical Epithelial Cells

2-82023 1 Kit Ask for price

Mouse Cervix PrimaCell4: Normal Endometrial Epithelial Cells

2-82026 1 Kit Ask for price

Mouse Colon PrimaCell: Normal Colorectal Epithelial Cells

2-82028 1 Kit Ask for price

Mouse Esophagus PrimaCell1: Normal Esophageal Epithelial Cells

2-82035 1 Kit Ask for price

Mouse Eye PrimaCell2: Normal Corneal Epithelial Cells

2-82038 1 Kit Ask for price

Mouse Eye PrimaCell4: Normal Lens Epithelial Cells

2-82040 1 Kit Ask for price

Mouse Kidney PrimaCell2: Normal Kidney Epithelial Cells

2-82057 1 Kit Ask for price

Mouse Ovary PrimaCell1: Normal Ovarian Epithelial Cells

2-82077 1 Kit Ask for price

Mouse Prostate PrimaCell: Normal Prostate Epithelial Cells

2-82083 1 Kit Ask for price

Mouse Ureter PrimaCell: Normal Ureter Epithelial Cells

2-82092 1 Kit Ask for price

Mouse Urethral PrimaCell: Normal Urethral Epithelial Cells

2-82093 1 Kit Ask for price

Mouse Pancreas PrimaCell 2: Pancreatic Epithelial Cells

2-82102 1 Kit Ask for price

Rat Bladder PrimaCell1: Normal Bladder Epithelial Cells

2-82506 1 Kit Ask for price

Rat Cervix PrimaCell1: Normal Cervical Epithelial Cells

2-82524 1 Kit Ask for price

Rat Cervix PrimaCell4: Normal Endometrial Epithelial Cells

2-82527 1 Kit Ask for price

Rat Colon PrimaCell1: Normal Colorectal Epithelial Cells

2-82529 1 Kit Ask for price

Rat Esophagus PrimaCell1: Normal Esophageal Epithelial Cells

2-82536 1 Kit Ask for price

Rat Eye PrimaCell2: Normal Corneal Epithelial Cells

2-82539 1 Kit Ask for price

Rat Eye PrimaCell4: Normal Lens Epithelial Cells

2-82541 1 Kit Ask for price

Rat Kidney PrimaCell2: Normal Kidney Epithelial Cells

2-82556 1 Kit Ask for price

Rat Ovary PrimaCell1: Normal Ovarian Epithelial Cells

2-82576 1 Kit Ask for price

Rat Prostate PrimaCell: Normal Prostate Epithelial Cells

2-82580 1 Kit Ask for price

Rat Ureter PrimaCell: Normal Ureter Epithelial Cells

2-82588 1 Kit Ask for price

Rat Urethral PrimaCell: Normal Urethral Epithelial Cells

2-82589 1 Kit Ask for price

Rat Pancreas PrimaCell 2: Pancreatic Epithelial Cells

2-82597 1 Kit Ask for price

Immortalized Baby Mouse Kidney Epithelial Cells (iBMK)

T0082 1x106 cells / 1.0 ml Ask for price

Immortalized Mouse Pancreas Epithelial Cells-Conditionally (IMPE)

T0156 1x106 cells / 1.0 ml Ask for price

Immortalized Rat Conjunctival Epithelial Cells (CJ4.1A) - SV40

T0368 1x106 cells / 1.0 ml Ask for price

Immortalized Rat Conjunctival Epithelial Cells (CJ4.3) - SV40

T0369 1x106 cells / 1.0 ml Ask for price

Immortalized Mouse Lung Epithelial Cells (MLE-15)

T0495 1x106 cells / 1.0 ml
EUR 686.4

Immortalized Mouse Colonic Epithelial Cells-Conditionally (YAMC)

T0567 1x106 cells / 1.0 ml Ask for price

Immortalized Mouse Stomach Epithelial Cells-Conditionally (ImSt)

T0569 1x106 cells / 1.0 ml Ask for price

Mouse Anti Human Fibroblasts/Epithelial Cells Monoclonal Antibody,RPE

DMABT-49595MH 25 TEST
EUR 514.8

Human Airway PrimaCell2: Normal Bronchial and Tracheal Epithelial Cells

2-96002 1 Kit Ask for price

Human Airway PrimaCell1: Normal Airway Epithelial Cells Growth Medium

9-46001 5 x 100 ml Ask for price

Human Bladder PrimaCell1: Normal Bladder Epithelial Cells Growth Medium

9-46007 5 x 100 ml Ask for price

Human Cervix PrimaCell1: Normal Cervical Epithelial Cells Growth Medium

9-46044 5 x 100 ml Ask for price

Human Cervix PrimaCell4: Normal Endometrial Epithelial Cells Growth Medium

9-46047 5 x 100 ml Ask for price

Human Esophagus PrimaCell1: Normal Esophageal Epithelial Cells Growth Medium

9-46055 5 x 100 ml Ask for price

Human Eye PrimaCell2: Normal Corneal Epithelial Cells Growth Medium

9-46058 5 x 100 ml Ask for price

Human Eye PrimaCell4: Normal Lens Epithelial Cells Growth Medium

9-46060 5 x 100 ml Ask for price

Human Kidney PrimaCell2: Normal Kidney Epithelial Cells Growth Medium

9-46075 5 x 100 ml Ask for price

Human Ovary PrimaCell1: Normal Ovarian Epithelial Cells Growth Medium

9-46094 5 x 100 ml Ask for price

Human Prostate PrimaCell: Normal Prostate Epithelial Cells Growth Medium

9-46099 5 x 100 ml Ask for price

Human Ureter PrimaCell2: Normal Ureter Epithelial Cells Growth Medium

9-46112 5 x 100 ml Ask for price

Human Urethral PrimaCell: Normal Urethral Epithelial Cells Growth Medium

9-46113 5 x 100 ml Ask for price

Human Colon PrimaCell: Normal Colorectal Epithelial Cells Growth Medium

9-46115 5 x 100 ml Ask for price

Human Thyroid PrimaCell: Normal Thyroid Epithelial Cells Growth Medium

9-46124 5 x 100 ml Ask for price

Human Prostate Tissue Preparation Buffer: Normal Prostate Epithelial Cells

9-80099 1 x 100 ml Ask for price

Human Urethral Tissue Preparation Buffer: Normal Urethral Epithelial Cells

9-80113 1 x 100 ml Ask for price

Human Colon Tissue Preparation Buffer: Normal Colorectal Epithelial Cells

9-80297 1 x 100 ml Ask for price

Human Thyroid Tissue Preparation Buffer: Normal Thyroid Epithelial Cells

9-80317 1 x 100 ml Ask for price

Immortalized Human Mammary Epithelial Progenitor (K5+/K19+) Cells - hTERT

T0452 1x106 cells / 1.0 ml Ask for price

Immortalized Human Endocervical Epithelial Cells- HPV E6/E7 (A2EN)

T0595 1x106 cells / 1.0 ml
EUR 825.6

Mouse Breast PrimaCell: Normal Mammary Epithelial Primary Cells

2-82021 1 Kit Ask for price

Mouse Eye PrimaCell5: Normal Retinal Pigment Epithelial Cells

2-82041 1 Kit Ask for price

Mouse Intestine PrimaCell3: Normal Intestinal Mucosa Epithelial Cells

2-82053 1 Kit Ask for price

Mouse Kidney PrimaCell8: Normal Renal Tubular Epithelial Cells

2-82063 1 Kit Ask for price

Mouse Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells

2-82088 1 Kit Ask for price

Mouse Lung PrimaCell 7: Alveolar Epithelial Cells II

2-82101 1 Kit Ask for price

Rat Breast PrimaCell: Normal Mammary Epithelial Primary Cells

2-82522 1 Kit Ask for price

Rat Eye PrimaCell5: Normal Retinal Pigment Epithelial Cells

2-82542 1 Kit Ask for price

Rat Intestine PrimaCell1: Normal Intestinal Mucosa Epithelial Cells

2-82552 1 Kit Ask for price

Rat Kidney PrimaCell8: Normal Renal Tubular Epithelial Cells

2-82562 1 Kit Ask for price

Rat Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells

2-82585 1 Kit Ask for price

Rat Lung PrimaCell 7: Alveolar Epithelial Cells II

2-82596 1 Kit Ask for price

Immortalized Mouse Small Intestinal Epithelial Cells-Conditionally (MSIE)

T0568 1x106 cells / 1.0 ml Ask for price

Immortalized Mouse Prolactin-Responsive Mammary Epithelial Cells (HC11)

T0672 1x106 cells / 1.0 ml Ask for price

Human Breast PrimaCell: Normal Mammary Epithelial Primary Cells Growth Medium

9-46023 5 x 100 ml Ask for price

Human Eye PrimaCell5: Normal Retinal Pigment Epithelial Cells Growth Medium

9-46061 5 x 100 ml Ask for price

Human Intestine PrimaCell1: Normal Intestinal Mucosa Epithelial Cells Growth Medium

9-46071 5 x 100 ml Ask for price

Human Kidney PrimaCell7: Normal Renal Tubular Epithelial Cells Growth Medium

9-46080 5 x 100 ml Ask for price

Human Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells Growth Medium

9-46104 5 x 100 ml Ask for price

Human Pancreas PrimaCell 2: Normal Pancreatic Epithelial Cells Growth Medium

9-46123 5 x 100 ml Ask for price

Human Airway Tissue Preparation Buffer 1: Normal Airway Epithelial Cells

9-80001 1 x 100 ml Ask for price

Human Bladder Tissue Preparation Buffer 1: Normal Bladder Epithelial Cells

9-80007 1 x 100 ml Ask for price

Human Breast Tissue Preparation Buffer: Normal Mammary Epithelial Primary Cells

9-80023 1 x 100 ml Ask for price

Human Cervix Tissue Preparation Buffer 1: Normal Cervical Epithelial Cells

9-80044 1 x 100 ml Ask for price

Human Cervix Tissue Preparation Buffer 4: Normal Endometrial Epithelial Cells

9-80047 1 x 100 ml Ask for price

Human Esophagus Tissue Preparation Buffer 1: Normal Esophageal Epithelial Cells

9-80055 1 x 100 ml Ask for price

Human Eye Tissue Preparation Buffer 2: Normal Corneal Epithelial Cells

9-80058 1 x 100 ml Ask for price

Human Eye Tissue Preparation Buffer 4: Normal Lens Epithelial Cells

9-80060 1 x 100 ml Ask for price

Human Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80075 1 x 100 ml Ask for price

Human Ovary Tissue Preparation Buffer 1: Normal Ovarian Epithelial Cells

9-80094 1 x 100 ml Ask for price

Human Stomach Tissue Preparation Buffer: Normal Stomach Mucosa Epithelial Cells

9-80104 1 x 100 ml Ask for price

Human Ureter Tissue Preparation Buffer 2: Normal Ureter Epithelial Cells

9-80112 1 x 100 ml Ask for price

Human Pancreas Tissue Preparation Buffer 2: Normal Pancreatic Epithelial Cells

9-80316 1 x 100 ml Ask for price

Mouse Airway PrimaCell1: Normal Bronchial and Tracheal Epithelial Cells

2-82001 1 Kit Ask for price

Rat Airway PrimaCell1: Normal Bronchial and Tracheal Epithelial Cells

2-82501 1 Kit Ask for price

Rat Bladder PrimaCell1: Normal Bladder Epithelial Cells Growth Medium

9-25006 5 x 100 ml Ask for price

Rat Cervix PrimaCell1: Normal Cervical Epithelial Cells Growth Medium

9-25024 5 x 100 ml Ask for price

Rat Cervix PrimaCell4: Normal Endometrial Epithelial Cells Growth Medium

9-25027 5 x 100 ml Ask for price

Rat Colon PrimaCell1: Normal Colorectal Epithelial Cells Growth Medium

9-25029 5 x 100 ml Ask for price

Rat Esophagus PrimaCell1: Normal Esophageal Epithelial Cells Growth Medium

9-25036 5 x 100 ml Ask for price

Rat Eye PrimaCell2: Normal Corneal Epithelial Cells Growth Medium

9-25039 5 x 100 ml Ask for price

Rat Eye PrimaCell4: Normal Lens Epithelial Cells Growth Medium

9-25041 5 x 100 ml Ask for price

Rat Kidney PrimaCell2: Normal Kidney Epithelial Cells Growth Medium

9-25056 5 x 100 ml Ask for price

Rat Ovary PrimaCell1: Normal Ovarian Epithelial Cells Growth Medium

9-25076 5 x 100 ml Ask for price

Human amniotic membranes have been used to thicken keratin networks. The human amniotic membrane in combination with Bilaminar and Flap Flap Techniques Coron has produced aesthetic and functionally satisfying results, covering the gingiva recession and restoring natural colors and gingival thickness. Bilaminar techniques with flap and cryopreserved cryopreserved cryopreserved membrane are a valid alternative in treating Gingival recession of Miller I and II classes and reducing biological morbidity of multiple interventions.

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