Experiments were carried out at ten Chiaa. 10% dimethylsulphoxide in hydroxyethyl 6% starch solution is used as cryoprotectant; All Chiaa is cooled at a level controlled and stored in the liquid nitrogen vapor phase (-194 ° C). Two Disbursement Protocols are tested: (1) placing Chiaa in a water bath at 37 ° C, and (2) Chiaa is disbursed in a controlled environment at 5 ° C. All samples undergo analysis under the scanning electron microscope. Testing of Chiaa’s mechanical properties is evaluated on a single-made strain test engine specifically made. Longitudinal and circular samples were prepared from each Chiaa tested.

Results: Ultrastructoral analysis revealed that the five Chiaa was disbursed during the Disbursement Protocol 1 which showed more damage to the subendotial structure when compared to the disbursed sample in the protocol 2. Mechanical properties: 1-Longitudinal Disbursement Protocol UTS 2, 53 ± 0, 47 MPa on relative strain 1, 27 ± 0, 12 and around UTS 1, 94 ± 0, 27 MPa on the relative strain 1, 33 ± 0, 09. Disbursement Protocol 2-Longitudinal Tensile Strain (UTS) 2, 42 ± 0, 34 MPa on relative strain 1, 32 ± 0, 09 and circular utiat 1, 98 ± 0, 26 MPa on the relative strain of 1, 29 ± 0, 07. Comparing UTS does not show statistical differences between disbursement methods.

Expand the use of test challenges as Biomarkers repair DNA in the human population: protocols for fresh blood samples and cryopreserved, and for different challenges agents

Deficiencies in DNA (DDRR) damage response (DDRR) can cause serious pathological results; Therefore, having the ability to determine individual DDRRs will increase concerns in the assessment of health risks and in determining individual responses to cancer therapy. However, most methods for evaluating DDRR are not fully suitable for population studies. Test challenges have received acceptance for this purpose. The test traditionally uses X-rays as a challenge agent and mononuclear cell peripheral blood isolated (PBMC) as cell specimens. To increase the use of the test, the purpose of this investigation is to use processed blood samples differently, to hire other challenges agents with various induction mechanisms of DNA damage / improvement, and to produce protocols to detect different DDRR capacities.

Fresh and frozen blood samples are challenged by bleomycin, methyl methanesulfonate (MMS) and ultraviolet light. Induction significant damage after all treatments, and progressive DDRR and depends on time observed. There was no significant difference obtained in the capacity of DDRR of blood samples all fresh or frozen compared to PBMC, except that fresh blood samples showed the capacity of DDRR induced by MMS higher than PBMC. The results of this study indicate that the test of comets can be used as routine biomarkers of DDRR capacity in human biomonitor studies, and that all blood is also a biomatrix that is useful for this test. Data collected allows us to recommend various protocols for test-comets that are useful for evaluating DDRR capacity in several main DNA repair lines. As a result, the usefulness of testing the challenges can be greatly expanded.

Micronucleus cytokinesis-block test on fresh blood mononuclear cells and isolated human cryopreserved

The Cytokinesis-Block Micronucleus (CBMN) test is a standard method used for genotoxicity studies. Conventional overall blood culture (WBC) is often used for this test, although this test can also be carried out on isolated blood mononuclear cell culture (PBMC). However, the standardization of the protocol for the CBMN PBMC test has not been widely investigated. The purpose of this study was to optimize the reliable CBMN test protocol for fresh peripheral blood mononuclear cells and cryopreserved (PBMC), and to compare the results of Micronuclei (MNI) between WBC and PBMC culture.

The CBMN G0 test is carried out in all blood, new isolated, and PBMC cryopreserved from healthy human blood samples and five samples of radiosensitive patients. Cells hit by 220 kv x-ray in vitro ranging from 0.5 to 2 gy. The Optimized PBMC CBMN test shows adequate repetition and small inter-individual variability. MNI value is significantly higher for WBC than for fresh PBMC. In addition, the Cryopreservation of PBMC resulted in an increase in significant MNI values, while different Cryopreservation time did not have a significant impact. In conclusion, our standard CBMN test on fresh PBMC and cryopreserved can be used for genotoxicity, biological dosimmetry, and radiosensitivity assessment.

Bilaminar Engineering with Corriocer Advanced Flap and Cryopreserved Human Amniotic Membranes in the treatment of gingiva recession

Gingiva recession is usually treated with surgical therapy that involves the use of connective network autograft to thicken gingiva network. It has an important biological impact on patients because they will have surgical wounds on two different oral cavity sites. In this case report, we offer an alternative technique for the treatment of Gingiva recession class Miller. A 40-year-old woman patient has been treated with a combination of Bilaminar and Flap Flap technique in a coron to cover the Miller I Gingiva recession with the addition of the cryopreserved human amniotic membrane.

Human Primary Nasal Epithelial Cells
T4014	ABM	5x10^5 cells / 1.0 ml	EUR 1375

Human Primary Thymic Epithelial Cells
T4980	ABM	5x10^5 cells / 1.0 ml	EUR 975

Human Primary Mammary Epithelial Cells
T4036	ABM	5x10^5 cells / 1.0 ml	EUR 625

Human Primary Bladder Epithelial Cells
T4161	ABM	5x10^5 cells / 1.0 ml	Ask for price

Human Primary Corneal Epithelial Cells
T4058	ABM	5x10^5 cells / 1.0 ml	EUR 1475

Primary Human Prostate Epithelial Cells
CSC-C9039J	Creative Bioarray	One Frozen vial	Ask for price

Human Primary Prostate Epithelial Cells
T4078	ABM	5x10^5 cells / 1.0 ml	EUR 625

Human Primary Amniotic Epithelial Cells
T4109	ABM	5x10^5 cells / 1.0 ml	EUR 625

Human Bronchial/Tracheal Epithelial Cells
ABC-TC3448	AcceGen	1 vial	Ask for price
Description: Human Bronchial/Tracheal Epithelial Cells, when grown in LIBro Medium, provide an ideal serum-free culture model for the accurate studies of toxicity, cystic fibrosis, asthma, pathogenesis, pharmacology or airway wound healing. HBTEC are cultured without retinoic acid and cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our HBTEC are quality tested in LIBro B/T Medium to ensure optimal growth over a period of at least 15 population doublings at rates equal to or greater than other commercially available media. Cell Features:HBTEC are cryopreserved as primary cells. Cells are isolated from human bronchi/trachea and expanded once in culture vessels before being harvested for cryopreservation.HSAEC are cryopreserved after primary cells are isolated from human lung tissue and expanded once in culture vessels before being harvested for cryopreservation. Human Airway Epithelial Cells are not exposed to retinoic acid during isolation or expansion.Human Airway Epithelial Cells are extensively tested for quality and optimal performance.Gentaur guarantees performance and quality.

Human Primary Placental Epithelial Cells
T4076	ABM	5x10^5 cells / 1.0 ml	EUR 625

Human Primary Bronchial Epithelial Cells
T4006	ABM	5x10^5 cells / 1.0 ml	EUR 625

Immortalized Human Tracheal Epithelial Cells
T0489	ABM	1x106 cells / 1.0 ml	EUR 2750

Human Primary Esophageal Epithelial Cells
T4230	ABM	5x10^5 cells / 1.0 ml	EUR 1375

Human Primary Small Airway Epithelial Cells
T4008	ABM	5x10^5 cells / 1.0 ml	EUR 625

C57BL/6 Mouse Primary Tracheal and Bronchial Epithelial Cells
ABC-TC4285	AcceGen	1 vial	Ask for price
Description: C57BL/6 Mouse Primary Tracheal and Bronchial Epithelial Cells from Gentaur are isolated from tracheal and bronchial tissue of pathogen-free laboratory mice. C57BL/6 Mouse Primary Tracheal and Bronchial Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur’ Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5×106 cells per ml and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM-1 (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.

Human Primary Mammary Epithelial Cells (HMEpC)
P0006038	Addexbio	One Frozen vial	EUR 600

Human Primary COPD Bronchial Epithelial Cells
T5429	ABM	5 x 10^5 / 1.0 ml	Ask for price

Human Primary Renal Cortical Epithelial Cells
T4088	ABM	5x10^5 cells / 1.0 ml	EUR 975

Human Primary Colonic Epithelial cells (HCoEpC)
P0009028	Addexbio	One Frozen vial	EUR 650

Normal Human Primary Broncial Epithelial Cells
T5511	ABM	5x10^5 cells / 1.0 ml	EUR 875

Human Primary Cervical Epithelial Cells (HCxEpC)
P0008008	Addexbio	One Frozen vial	EUR 650

Human Primary Prostate Epithelial Cells (HPrEpC)
P0019010	Addexbio	One Frozen vial	EUR 700

Human Primary Ovarian Surface Epithelial Cells
T4198	ABM	5x10^5 cells / 1.0 ml	EUR 975

Human Primary Retinal Pigment Epithelial Cells
T4057	ABM	5x10^5 cells / 1.0 ml	EUR 1375

Human Airway PrimaCell™2: Normal Bronchial and Tracheal Epithelial Cells Growth Medium
9-46002	CHI Scientific	5 x 100 ml	EUR 151.2

Human Primary Asthma Bronchial Epithelial Cells
T5463	ABM	1 x 10^6 / 1.0 ml	Ask for price

Normal Human Primary Bronchial Epithelial Cells
T5510	ABM	5x10^5 cells / 1.0 ml	EUR 875

Human Primary Alveolar Epithelial Cells - Type II
T4116	ABM	5x10^5 cells / 1.0 ml	EUR 3695

Human Pulmonary Bronchial/Tracheal Epithelial Cells
cAP-0124	Angio Proteomie	1Frozen Vial	EUR 594

Rat Tracheal Epithelial Cells
ABC-TC4233	AcceGen	1 vial	Ask for price
Description: Rat Primary Tracheal Epithelial Cells from Gentaur are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Primary Tracheal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen.Rat Primary Tracheal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Primary Tracheal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Human Primary Emphysema Bronchial Epithelial Cells
T5473	ABM	5 x 10^5 / 1.0 ml	Ask for price

Normal Human Primary Small Airway Epithelial Cells
T5515	ABM	5x10^5 cells / 1.0 ml	EUR 875

Human Thyroid PrimaCell™: Thyroid Epithelial Cells
2-96124	CHI Scientific	1 Kit	EUR 1132.11

Rat Airway PrimaCell™1: Normal Bronchial and Tracheal Epithelial Cells
2-82501	CHI Scientific	1 Kit	EUR 943.11

Normal Human Primary Intestinal Epithelial Cells Ilium
T5528	ABM	5x10^5 cells / 1.0 ml	EUR 875

Human Primary Intra-Hepatic Biliary Epithelial Cells
T4221	ABM	5x10^5 cells / 1.0 ml	Ask for price

Mouse Airway PrimaCell™1: Normal Bronchial and Tracheal Epithelial Cells
2-82001	CHI Scientific	1 Kit	EUR 943.11

Normal Human Primary Intestinal Epithelial Cells Jejunum
T5527	ABM	5x10^5 cells / 1.0 ml	EUR 875

Rat Primary Lung Epithelial Cells
T4800	ABM	5x10^5 cells / 1.0 ml	EUR 625

Normal Human Primary Intestinal Epithelial Cells Duodenem
T5526	ABM	5x10^5 cells / 1.0 ml	EUR 875

Human Eye PrimaCell™4: Normal Lens Epithelial Cells
2-96060	CHI Scientific	1 Kit	EUR 1132.11

Rat Primary Renal Epithelial Cells
T4936	ABM	5x10^5 cells / 1.0 ml	Ask for price

Human Lung PrimaCell™ 7: Alveolar Epithelial Cells II
2-96122	CHI Scientific	1 Kit	EUR 1132.11

Human Primary Pulmonary Fibrosis Bronchial Epithelial Cells
T5462	ABM	1 x 10^6 / 1.0 ml	Ask for price

Normal Human Primary Intestinal Epithelial Cells Transverse Colon
T5524	ABM	5x10^5 cells / 1.0 ml	EUR 975

Human Pancreas PrimaCell™ 2: Pancreatic Epithelial Cells
2-96123	CHI Scientific	1 Kit	EUR 1132.11

Rat Primary Bladder Epithelial Cells
T4940	ABM	5x10^5 cells / 1.0 ml	Ask for price

Human Eye PrimaCell™2: Normal Corneal Epithelial Cells
2-96058	CHI Scientific	1 Kit	EUR 1132.11

Human Colon PrimaCell™: Normal Colorectal Epithelial Cells
2-96115	CHI Scientific	1 Kit	EUR 1132.11

Rat Primary Cervical Epithelial Cells
T4952	ABM	5x10^5 cells / 1.0 ml	Ask for price

Rat Primary Prostate Epithelial Cells
T4956	ABM	5x10^5 cells / 1.0 ml	Ask for price

Human Prostate PrimaCell™: Normal Prostate Epithelial Cells
2-96099	CHI Scientific	1 Kit	EUR 1132.11

Human Urethral PrimaCell™: Normal Urethral Epithelial Cells
2-96113	CHI Scientific	1 Kit	EUR 1132.11

Rat Primary Bronchial Epithelial Cells
T4803	ABM	5x10^5 cells / 1.0 ml	Ask for price

Mouse Primary Corneal Epithelial Cells
T4571	ABM	5x10^5 cells / 1.0 ml	EUR 1375

Mouse Primary Bladder Epithelial Cells
T4640	ABM	5x10^5 cells / 1.0 ml	EUR 975

Human Airway PrimaCell™1: Normal Airway Epithelial Cells
2-96001	CHI Scientific	1 Kit	EUR 1132.11

Human Kidney PrimaCell™2: Normal Kidney Epithelial Cells
2-96075	CHI Scientific	1 Kit	EUR 1132.11

Human Ovary PrimaCell™1: Normal Ovarian Epithelial Cells
2-96094	CHI Scientific	1 Kit	EUR 1228.5

Human Ureter PrimaCell™2: Normal Ureter Epithelial Cells
2-96112	CHI Scientific	1 Kit	EUR 1132.11

ACE2-GFP Human Pulmonary Bronchial/Tracheal Epithelial Cells
HPEC003	Neuromics	each	EUR 1165.32

Rat Primary Esophageal Epithelial Cells
T4912	ABM	5x10^5 cells / 1.0 ml	Ask for price

Mouse Primary Cervical Epithelial Cells
T4650	ABM	5x10^5 cells / 1.0 ml	Ask for price

Mouse Primary Prostate Epithelial Cells
T4654	ABM	5x10^5 cells / 1.0 ml	Ask for price

BALB/c Mouse Tracheal Epithelial Cells
ABC-TC3108	AcceGen	1 vial	Ask for price
Description: BALB/c Mouse Primary Tracheal Epithelial Cells from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Tracheal Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Culture Complete Growth Medium for 3-7 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Primary Tracheal Epithelial Cells can be used in assays of cell to cell adhesion and migration. Standard biochemical procedures performed with epithelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Human Bladder PrimaCell™1: Normal Bladder Epithelial Cells
2-96007	CHI Scientific	1 Kit	EUR 1228.5

Human Cervix PrimaCell™1: Normal Cervical Epithelial Cells
2-96044	CHI Scientific	1 Kit	EUR 1132.11

C57BL/6 Mouse Tracheal Epithelial Cells
ABC-TC3256	AcceGen	1 vial	Ask for price
Description: C57BL/6 Mouse Primary Tracheal Epithelial Cells from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Tracheal Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Culture Complete Growth Medium for 3-7 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Primary Tracheal Epithelial Cells can be used in assays of cell to cell adhesion and migration. Standard biochemical procedures performed with epithelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Normal Human Primary Intestinal Epithelial Cells Ascending Colon
T5523	ABM	5x10^5 cells / 1.0 ml	EUR 975

Mouse Primary Esophageal Epithelial Cells
T4612	ABM	5x10^5 cells / 1.0 ml	Ask for price

Rat Airway PrimaCell™1: Normal Bronchial and Tracheal Epithelial Cells Growth Medium
9-25001	CHI Scientific	5 x 100 ml	EUR 122.85

Normal Human Primary Intestinal Epithelial Cells Descending Colon
T5525	ABM	5x10^5 cells / 1.0 ml	EUR 975

Human Cervix PrimaCell™4: Normal Endometrial Epithelial Cells
2-96047	CHI Scientific	1 Kit	EUR 1228.5

Human Stomach PrimaCell™: Normal Stomach Mucosa Epithelial Cells
2-96104	CHI Scientific	1 Kit	EUR 1132.11

Human Primary ∆F508/∆1152H Cystic Fibrosis Bronchial Epithelial Cells
T5425	ABM	5 x 10^5 / 1.0 ml	Ask for price

Primary Rabbit Thymic Epithelial Cells (TEC)
CSI116Rb01	Cloud-Clone	5X10^5	EUR 1160

Mouse Airway PrimaCell™1: Normal Bronchial and Tracheal Epithelial Cells Growth Medium
9-32001	CHI Scientific	5 x 100 ml	EUR 122.85

Human Breast PrimaCell™: Normal Mammary Epithelial Primary Cells
2-96023	CHI Scientific	1 Kit	EUR 1228.5

Human Esophagus PrimaCell™1: Normal Esophageal Epithelial Cells
2-96055	CHI Scientific	1 Kit	EUR 1228.5

Human Eye PrimaCell™5: Normal Retinal Pigment Epithelial Cells
2-96061	CHI Scientific	1 Kit	EUR 1228.5

Primary Rabbit Corneal Epithelial Cells (CEC)
CSI019Rb01	Cloud-Clone	5X10^5	EUR 1670

Primary Rat Esophageal Epithelial Cells (EEC)
CSI105Ra01	Cloud-Clone	5X10^5	EUR 850

Human Lens Epithelial Cells
ABC-TC3669	AcceGen	1 vial	Ask for price
Description: Human Lens Epithelial Cells from Gentaur Research Laboratories are isolated from the human lens. HLEpiC are cryopreserved at primary culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HLEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, cytokeratin-19 and fibronectin. HLEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HLEpiC are guaranteed to further culture in the conditions provided by Gentaur Research Laboratories.

Human Oral Epithelial Cells
ABC-TC4365	AcceGen	1 vial	Ask for price
Description: Cells are only guaranteed with purchase of Gentaur Media, Extra Cellular Matrix, Trypsin EDTA, 1X PBS, and freezing media for the appropriate cell culture, for 30 days from the date of shipment.

Human Lens Epithelial Cells
CSC-7764W	Creative Bioarray	One Frozen vial	Ask for price

Human Kidney PrimaCell™7: Normal Renal Tubular Epithelial Cells
2-96080	CHI Scientific	1 Kit	EUR 1228.5

Rat Primary Ovarian Surface Epithelial Cells
T4955	ABM	5x10^5 cells / 1.0 ml	Ask for price

Human Nasal Epithelial Cells
ABC-TC3719	AcceGen	1 vial	Ask for price
Description: The cells are isolated from normal human adult tissues of the airways (nasal mucosa, trachea, bronchi, and distal respiratory tract), breast (mammary gland), kidney (whole kidney and renal cortex), and the placenta (amniotic membrane). Shortly after isolation, all Gentaur Human Epithelial Cells are cryopreserved at passage 2 (P2) . Each cryo vial contains more than 500,000 viable cells after thawing. Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

Human Renal Epithelial Cells
ABC-TC3782	AcceGen	1 vial	Ask for price
Description: Human Renal Epithelial Cells(Human kidney epithelial cells) are derived from whole kidneys that have been dissociated into single cells and purified in culture. Human kidney epithelial cells are from a single donor. These are highly purified epithelial cells as determined by specific epithelial cell markers. Human kidney epithelial cells enable researchers to study the development and biochemical function of the kidneys. In addition, they are an ideal cell for studying absorption and secretion that occurs in the kidneys. Development period: Prenatal

Human Nasal Epithelial Cells
CSC-C1511	Creative Bioarray	One Frozen vial	Ask for price

Human Renal Epithelial Cells
ALHE35	Alphabioregen	1ml frozen Vial	EUR 620

Human Primary DF508/DF508 Cystic Fibrosis Bronchial Epithelial Cells
T5423	ABM	5 x 10^5 / 1.0 ml	Ask for price

Human Primary DF508/N1303K Cystic Fibrosis Bronchial Epithelial Cells
T5424	ABM	5 x 10^5 / 1.0 ml	Ask for price

Human Primary DF508/S549N Cystic Fibrosis Bronchial Epithelial Cells
T5427	ABM	1 x 10^6 / 1.0 ml	Ask for price

Human Tonsil Epithelial Cells
ABC-TC3834	AcceGen	1 vial	Ask for price
Description: Tonsil epithelium is a heterogeneous lymphoid organ containing two different types of actively differentiating epithelia, the lining stratified squamous epithelium and the reticulated crypt, or lymphoepithelium. The tonsil crypts represent a specialized compartment, important in the immunological functions of the tonsil because of the immediate proximity of the epithelial and lymphoid tissues. In Situ staining of tonsil epithelium suggests that expression of the simple epithelial keratins K8, K18 and K19 is located to the crypt epithelial cells. Cell culture of tonsil epithelium produces heterogeneous squamous epithelial culture in which differentiation appears to be ongoing and keratin expression patterns are consistent with those found in tonsil epithelial cells in situ. HTonEpiC from Gentaur Research Laboratories are isolated from human normal tonsil tissue. HTonEpiC are cryopreserved at passage one culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HTonEpiC are characterized by immunofluorescent method with cytokeratine antibodies. HTonEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HTonEpiC are guaranteed to further culture at the conditions provided by Gentaur Research Laboratories.

Human Thymic Epithelial Cells
ABC-TC5517	AcceGen	1 vial	Ask for price
Description: Thymic epithelial cells (TyEpiC) are vital regulators of thymocyte development and T lymphocyte (T-cell) tolerance. The thymus contains two types of TyEpiC, cortical and medullary thymic epithelial cells, which regulate the positive selection of thymocytes and the negative selection of autoreactive T-cells. The inability of TyEpiC to remove autoreactive T-cells in the thymus can affect self-tolerance and contribute to the development of autoimmune diseases such as myasthenia gravis, type 1 diabetes, rheumatoid arthritis, and multiple sclerosis [3]. Studies have shown that changes in the expression of tumor necrosis factor receptor family members and transcription factors Foxn1 and autoimmune regulator (Aire) can alter the thymic microenvironment and self-tolerance. Human TyEpiC are an excellent in vitro model to study the mechanisms that modulate TyEpiC functionality and to develop targeted treatments for autoimmune disorders.

Normal Human Primary Intrahepatic Biliary Epithelial Cells (Cholagioctyes)
T5555	ABM	1x10^6 cells / 1.0 ml	EUR 1085

Primary Rabbit Esophageal Epithelial Cells (EEC)
CSI105Rb01	Cloud-Clone	5X10^5	EUR 1260

Human Colonic Epithelial Cells
ABC-TC3570	AcceGen	1 vial	Ask for price
Description: The colorectum is a major organ for both malignant and nonmalignant diseases. Cells that line the colonic mucosal surface form a major mechanical barrier that separates the host’s internal milieu from the external environment. In addition to the well-established role of epithelial cells in ion transport, these cells appear to function as an integral component of the mucosal immune system. Human colon epithelial cells can process and present antigens to T cells in vitro, and can be stimulated to express HLA class II and intercellular adhesion molecules in vivo. They also respond to a broad array of cytokines with altered gene expression and growth characteristics. In addition, normal colonic epithelial cells were found to express the integrin α3, α5, α6, β1 and β4 chains. HCoEpiC from Gentaur Research Laboratories are isolated from human colonic tissue. HCoEpiC are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HCoEpiC are characterized by immunofluorescent method with antibodies to CK18 and CK19. HCoEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCoEpiC are guaranteed to further culture for 15 population doublings in the conditions provided by Gentaur Research Laboratories.

Human Corneal Epithelial Cells
ABC-TC3577	AcceGen	1 vial	Ask for price
Description: The cornea is a unique tissue for two reasons: the transparency function and the synthesis of most of its proteins due to the cornea being an avascular tissue. The cornea consists of three distinct cell layers, the outer epithelium, the inner endothelium and the central stroma (the keratocytes). The corneal epithelium plays a role in the innate immune response by sensing the presence of pathogens and providing signals that activate the corneal defense system. It also highly expresses aldehyde dehydrogenase to protect against UV- and 4-hydroxynonenal-induced cellular damage. Corneal epithelial basal cell proliferation is controlled by a host of cytokines, e.g., epidermal growth factor, that activate their cognate receptors in the deeper layers of the epithelium. Instead of testing products in the eyes of animals, cultured corneal epithelial cells could be used for toxicology tests in in vitro models and for studying the molecular mechanisms involved in regulating human corneal epithelial cell differentiation.HCEpiC from Gentaur Research Laboratories are isolated from human cornea. HCEpiC are cryopreserved from passage one culture. Each vial contains 5×10^5 cells in 1 ml volume. HCEpiC are characterized by their cobble stone morphology in serum-free culture and immunofluorescent method with antibody to CK-18 and -19. HCEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCEpiC are guaranteed to further expand for 10 population doublings at the condition provided by Gentaur Research Laboratories.

Human Urothel Epithelial Cells
ABC-TC3860	AcceGen	1 vial	Ask for price
Description: The HUEPC cultures are produced on the base of human tissues. The HUEPC are isolated according to known procedures. Then primary cultures are produced. After further culturing, the HUEPC are frozen in a computer controlled freezing unit.

Human Bladder Epithelial Cells
ABC-TC4367	AcceGen	1 vial	Ask for price
Description: Human Primary Bladder Epithelial Cells from Gentaur are isolated from normal human bladder tissue. Human Primary Bladder Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaurandrsquo; Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5×10^6 cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.

Human Stomach Epithelial Cells
ABC-HP021X	AcceGen	1 vial	Ask for price
Description: Human Stomach Epithelial Cells are isolated from normal human stomach tissue. Cells are cryopreserved at passage 3 and delivered frozen.

Human Stomach Epithelial Cells
CSC-C9381W	Creative Bioarray	One Frozen vial	Ask for price

Human Colonic Epithelial Cells
CSC-C1488	Creative Bioarray	One Frozen vial	Ask for price

Human Bladder Epithelial Cells
ALHE36	Alphabioregen	1ml frozen Vial	EUR 620

Human Corneal Epithelial Cells
cAP-0211	Angio Proteomie	1Frozen Vial	EUR 594

Human Airway PrimaCell™2: Normal Bronchial and Tracheal Epithelial Cells Growth Supplements with Serum (for 500 ml medium)
9-47002	CHI Scientific	1 Set	EUR 321.3

Immortalized Human Cystic Fibrosis Tracheobronchial Epithelial Cells (CFT1)
T0681	ABM	1x106 cells / 1.0 ml	EUR 686.4

Human Eye PrimaCell™4: Normal Lens Epithelial Cells Growth Medium
9-46060	CHI Scientific	5 x 100 ml	EUR 151.2

Human Amniotic Epithelial Cells
ABC-TC3486	AcceGen	1 vial	Ask for price
Description: Human amniotic membrane is composed of an epithelial cell layer, a basement membrane and an avascular matrix. The amniotic epithelial cells (AEC) are formed from epiblasts on the 8th day after fertilization. A probable result of their embryonic origin, AEC lack major histocompatibility complex antigens and have been used for allotranplantation to treat patients with lysosomal diseases. Studies have shown that AEC have multiple functions such as synthesis and release of acetylcholine and catecholamine as well as expressing mRNA coding for DA receptors and DA transporters. They express neuronal and glial cell marks, produce basic fibroblast growth factors and hepatocyte growth factors and transform growth factor-beta. Human AEC has been suggested as an appropriate human cell model for studying DA release and uptake processes, receptor signal transduction and exploring newly developed drugs acting at these receptors.HAEpiC from Gentaur Research Laboratories are isolated from human amniotic membranes. HAEpiC are cryopreserved at passage one and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HAEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, -19 and vimentin. HAEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAEpiC are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories.

Human Prostate Epithelial Cells
ABC-TC3995	AcceGen	1 vial	Ask for price
Description: Human Prostate Epithelial Cells (HPrEC), when grown in LIPro Medium, provides an ideal serum-free culture model, without retinoic acid, for the study of prostate cells. Common uses for HPrEC are studies of hormonal regulation of the prostate, regulation and control of the secretory function of prostate cells and as a control for the study of prostate cancer. HPrEC are cryopreserved as secondary cells to ensure the highest viability and plating efficiency. Our HPrEC are quality tested in LIPro Medium to ensure optimal serum-free growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented media. Cell Features:HPrEC are cryopreserved as secondary cells, e.g. cells are isolated fromhuman prostate tissue and expanded twice in culture vessels before beingharvested for cryopreservation.HPrEC proliferate in a serum-free, retinoic acid-free medium withoutphenol red or antimicrobials when cultured in ProstaLifeTM medium.HPrEC are extensively tested for quality and optimal performance.Gentaur guarantees performance and quality.

Human Cervical Epithelial Cells
ABC-TC5466	AcceGen	1 vial	Ask for price
Description: Cervical Epithelial Cells have been isolated, plated, and expanded in culture vessels three times before being harvested for cryopreserved to ensure the highest purity, viability and plating efficiency. HCxEC are quality tested in Epithelial Cell Medium to ensure optimal morphology and growth over a period of at least 5 population doublings. Cervical Epithelial Cells are not exposed to antimicrobials or phenol red when cultured in Epithelial Cell Medium. Gentaur offers antimicrobials and phenol red, but they are not required for eukaryotic cell proliferation.

Human Alveolar Epithelial Cells
CSC-7845W	Creative Bioarray	One Frozen vial	Ask for price

Human Prostate Epithelial Cells
ALHE25	Alphabioregen	1ml frozen Vial	EUR 620

Human Alveolar Epithelial Cells
cAP-0208	Angio Proteomie	1Frozen Vial	EUR 643.5

ACE2-GFP Expressing Human Pulmonary Bronchial/Tracheal Epithelial Cells
cAP-0124ACE2-GFP	Angio Proteomie	1Frozen Vial	EUR 841.5

C57BL/6 Mouse Primary Thymus Epithelial Cells
ABC-TC5502	AcceGen	1 vial	Ask for price
Description: C57BL/6 Mouse Primary Thymus Epithelial Cells are isolated from thymus tissue of pathogen-free laboratory mice. C57BL/6 Mouse Primary Thymus Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur's Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM-1 (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.

Human Bronchial Epithelial Cells
ABC-TC3528	AcceGen	1 vial	Ask for price
Description: The epithelium is the interface between the body and the external environment and covers all exterior surfaces and interior lumina. Depending on the tissue of origin, the functions of epithelial cells are diverse and include absorption, secretion, protection, transcellular transport, and sensation.Gentaur offers a range of Epithelial Cells produced at Gentaur's cell culture facility. The cells are isolated from normal human adult tissues of the airways (nasal mucosa, trachea, bronchi, and distal respiratory tract), breast (mammary gland), kidney (whole kidney and renal cortex), and the placenta (amniotic membrane).Shortly after isolation, all Gentaur Human Epithelial Cells are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

Human Placental Epithelial Cells
ABC-TC3753	AcceGen	1 vial	Ask for price
Description: The epithelium is the interface between the body and the external environment and covers all exterior surfaces and interior lumina. Depending on the tissue of origin, the functions of epithelial cells are diverse and include absorption, secretion, protection, transcellular transport, and sensation.Gentaur offers a range of Epithelial Cells produced at Gentaur's cell culture facility. The cells are isolated from normal human adult tissues of the airways (nasal mucosa, trachea, bronchi, and distal respiratory tract), breast (mammary gland), kidney (whole kidney and renal cortex), and the placenta (amniotic membrane).Shortly after isolation, all Gentaur Human Epithelial Cells are cryopreserved at passage 2 (P2) using Gentaur's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Gentaur.

Human Laryngeal Epithelial Cells
ABC-TC5518	AcceGen	1 vial	Ask for price
Description: Laryngeal Epithelial Cells are cultured without retinoic acid and cryopreserved as primary cells to ensure the highest viability and plating efficiency.

Human Bronchial Epithelial Cells
CSC-C4388X	Creative Bioarray	One Frozen vial	Ask for price

Human Bronchial Epithelial Cells
ALHE47	Alphabioregen	1ml frozen Vial	EUR 620

Immortalized Human Cystic Fibrosis Tracheobronchial Epithelial Cells-SV40
CSC-I2271Z	Creative Bioarray	One Frozen vial	Ask for price

Human Thyroid PrimaCell™: Normal Thyroid Epithelial Cells Growth Medium
9-46124	CHI Scientific	5 x 100 ml	EUR 151.2

Human Esophageal Epithelial Cells
ABC-TC3612	AcceGen	1 vial	Ask for price
Description: The human esophagus is lined by a non-keratinizing, moist stratified squamous epithelium whose apical cell membranes and intercellular junctional complexes combine to produce an effective permeability barrier against the influx of luminal content. In particular, the barrier created by these structures limits exposure of the surface cells' basolateral cell membranes and entire membrane of cells of the deeper layers to the wide swings in osmolality occurring regularly within the esophageal lumen. Histologically, the esophageal epithelium consists of two zones, the basal and differentiated zones. Cellular proliferation is limited to the basal zone, and cells are thought to migrate from this area towards the esophageal lumen. Migration is associated with the initiation of differentiation and the sequential expression of differentiation markers. The availability of human esophageal epithelial cell culture provides an excellent in vitro model in the study of the physiology of esophageal epithelium and the mechanisms of the esophageal carcinogenesis.HEEC from Gentaur Research Laboratories are isolated from the human esophagus. HEEC are cryopreserved on passage one culture and delivered frozen. Each vial contains 5×10^5 cells in 1 ml volume. HEEC are characterized by immunofluorescent method with antibodies to cytokeratine-8, -18 and -19. HEEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEEC are guaranteed to further expand for 15 population doublings in the condition provided by Gentaur Research Laboratories.

Human Pancreatic Epithelial Cells
ABC-TC3744	AcceGen	1 vial	Ask for price
Description: Human Pancreatic Epithelial Cell were initiated by elutriation of dispase dissociated normal pancreatic tissue.These cells were originated using Complete Serum-Free Medium Kit With AcceSup™, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5×10^6 cells. This vial will initiate a Passage 4 cell culture in a 75cm2 flask.

Human Intestinal Epithelial Cells
ABC-TC3879	AcceGen	1 vial	Ask for price
Description: Cryopreserved InEpC are truly primary cells representing both villi (enterocytes, goblet, and enteroendocrine cells) and crypts structures. In combination with human intestinal myofibroblasts (InMyoFib).

Human Esophageal Epithelial Cells
CSC-7783W	Creative Bioarray	One Frozen vial	Ask for price

Human Pancreatic Epithelial Cells
CSC-8246W	Creative Bioarray	One Frozen vial	Ask for price

Primary Rat Renal Tubular Epithelial Cells (RTEC)
CSI061Ra01	Cloud-Clone	5X10^5	EUR 980

Human Eye PrimaCell™2: Normal Corneal Epithelial Cells Growth Medium
9-46058	CHI Scientific	5 x 100 ml	EUR 151.2

Human Primary DF508/621+1G>T Cystic Fibrosis Bronchial Epithelial Cells
T5426	ABM	5 x 10^5 / 1.0 ml	Ask for price

Human Endometrial Epithelial Cells
ABC-TC4601	AcceGen	1 vial	Ask for price
Description: Proliferation and Morphology: Normal morphology for 5 population doublings, minimum 70% viability when thawed from cryopreservation. Normal Human Endometrial (Uterine) Epithelial Cells provide an ideal serum-free culture model for many areas of research. These cells may be used to study cellular physiology of the reproductive tract, the cellular response to infectious agents, and other areas of research, Endometrial Epithelial Cells have been isolated, plated, and expanded in culture vessels three times before being harvested for cryopreservation to ensure the highest purity, viability and plating efficiency.

Primary Rat Renal Cortical Epithelial Cells (RCEC)
CSI067Ra01	Cloud-Clone	5X10^5	EUR 830

Human Airway PrimaCell™1: Normal Airway Epithelial Cells Growth Medium
9-46001	CHI Scientific	5 x 100 ml	EUR 151.2

Human Kidney PrimaCell™2: Normal Kidney Epithelial Cells Growth Medium
9-46075	CHI Scientific	5 x 100 ml	EUR 151.2

Human Ovary PrimaCell™1: Normal Ovarian Epithelial Cells Growth Medium
9-46094	CHI Scientific	5 x 100 ml	EUR 151.2

Human Ureter PrimaCell™2: Normal Ureter Epithelial Cells Growth Medium
9-46112	CHI Scientific	5 x 100 ml	EUR 151.2

Human Conjunctival Epithelial Cells
ABC-TC3573	AcceGen	1 vial	Ask for price
Description: The conjunctival epithelium is a stratified, non-keratinized epithelium. It can be distinguished by the corneal epithelium by the expression of different cytokeratins, mucins and by the presence of glycocalix. Decrease or loss of mucin/glycocalix production generates squamous metaplasia, which may lead to dry eye and ocular surface diseases. Human conjunctival epithelial cells represent potential target cells for mast cell mediators. They respond to histamine by generating inositol phosphates, mobilizing intracellular calcium, and then secreting proinflammatory cytokines like interleukin-6 and interleukin-8.HConEC from Gentaur Research Laboratories are isolated from human conjunctiva. HConEC are cryopreserved from passage one culture. Each vial contains 5×10^5 cells in 1 ml volume. HConEC are characterized by their cobble stone morphology in serum-free culture and immunofluorescent method with antibody to CK-18 and -19. HConEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HConEC are guaranteed to further expand for 10 population doublings at the condition provided by Gentaur Research Laboratories.

Human Conjunctival Epithelial Cells
CSC-7802W	Creative Bioarray	One Frozen vial	Ask for price

Human Prostate PrimaCell™: Normal Prostate Epithelial Cells Growth Medium
9-46099	CHI Scientific	5 x 100 ml	EUR 151.2

Human Urethral PrimaCell™: Normal Urethral Epithelial Cells Growth Medium
9-46113	CHI Scientific	5 x 100 ml	EUR 151.2

Human Colon PrimaCell™: Normal Colorectal Epithelial Cells Growth Medium
9-46115	CHI Scientific	5 x 100 ml	EUR 151.2

Human Intestine PrimaCell™1: Normal Intestinal Mucosa Epithelial Cells
2-96071	CHI Scientific	1 Kit	EUR 1132.11

Rat Thyroid PrimaCell™: Thyroid Epithelial Cells
2-82598	CHI Scientific	1 Kit	EUR 1039.5

Primary Mouse Renal Tubular Epithelial Cells (RTEC)
CSI061Mu01	Cloud-Clone	5X10^5	EUR 960

Immortalized Rat Tracheal Epithelial Cells (SPOC-1)
T0733	ABM	1x10^6 cells / 1.0 ml	EUR 3950

Primary Canine Renal Tubular Epithelial Cells (RTEC)
CSI061Ca01	Cloud-Clone	5X10^5	EUR 1250

Primary Rabbit Renal Tubular Epithelial Cells (RTEC)
CSI061Rb01	Cloud-Clone	5X10^5	EUR 1260

Primary Mouse Renal Cortical Epithelial Cells (RCEC)
CSI067Mu01	Cloud-Clone	5X10^5	EUR 810

Human Nasopharyngeal Epithelial Cells
cAP-0222	Angio Proteomie	1Frozen Vial	EUR 4455

Human Bladder PrimaCell™1: Normal Bladder Epithelial Cells Growth Medium
9-46007	CHI Scientific	5 x 100 ml	EUR 151.2

Human Cervix PrimaCell™1: Normal Cervical Epithelial Cells Growth Medium
9-46044	CHI Scientific	5 x 100 ml	EUR 151.2

Mouse Thyroid PrimaCell™: Thyroid Epithelial Cells
2-82103	CHI Scientific	1 Kit	EUR 1039.5

Primary Caprine Renal Tubular Epithelial Cells (RTEC)
CSI061Cp01	Cloud-Clone	5X10^5	EUR 1360

Human amniotic membranes have been used to thicken keratin networks. The human amniotic membrane in combination with Bilaminar and Flap Flap Techniques Coron has produced aesthetic and functionally satisfying results, covering the gingiva recession and restoring natural colors and gingival thickness. Bilaminar techniques with flap and cryopreserved cryopreserved cryopreserved membrane are a valid alternative in treating Gingival recession of Miller I and II classes and reducing biological morbidity of multiple interventions.