Inhibitory Effect of Tetramerized Single-Chain Variable Fragment of Anti-Cyclic Citrullinated Peptide Antibodies on the Proliferation, Activation, and Secretion of Cytokines of Fibroblast-Like Synoviocytes in Rheumatoid Arthritis In Vitro Co-Culture System
Tetramerized single chain variable fragment (scFv) anti-cyclic citrullinated peptide (TeAb-CCP) is built tetramerized scFv anti-cyclic peptide citrullinated (CCP) antibody to p53 domain tetrameric, aimed to investigate the effect on fibroblast-like synoviocytes (FLSs) proliferation, migration, invasion, and production of inflammatory mediators in the co-culture system in vitro peripheral mononuclear cells (PBMC) and FLSs. TeAb-CCP constructed by modifying the monovalent scFv antibodies to PKC with tetrameric domain p53 to increase affinity. FLSs isolated and cultured from rheumatoid arthritis (RA) patients and control subjects.
A co-culture system peripheral mononuclear cells (PBMC) and FLSs used. FLSs proliferation, migration and invasion were measured by MTT, the initial tests, and Transwell chamber. The supernatant was measured for cytokines, chemokines, metalloproteinases, and anti-CCP antibodies by Luminex liquid phase protein chip and ELISA. TeAb-CCP FLSs significantly inhibit proliferation in a dose-dependent fashion, with maximal action at a concentration of 100 mg / ml on day 7 in a co-culture with PBMCs and FLSs, but not the same as just FLSs.
TeAb-CCP was significantly suppressed FLSs migration and invasive ability compared with controls. Significantly lower levels of interleukin (IL) -6, IL-8, RANKL, a protein arginine deiminase (PAD) -2, PAD4, metalloproteinase (MMP) -1 and MMP-3 and anti-CCP antibodies found in the supernatant co -budaya TeAb-CCP group. Instead, transforming growth factor-β (TGF-β) and tissue inhibitor of metalloproteinase-2 (TIMP-2) increased significantly in the group TeAb-CCP.
There is no significant difference of IL-1a, IL-10, IL-17, TNF, VEGF and FGF was found between the two groups. As a blocking antibody, TeAb-CCP can significantly inhibit RA PBMC to produce pro-inflammatory mediators, and furthermore, inhibit proliferation, activation, migration, and invasion in vitro FLSs. In turn, it is suggested that a modified self citrullinated epitopes may be a new target for the treatment of RA.
Inhibitory Effect of Tetramerized Single-Chain Variable Fragment of Anti-Cyclic Citrullinated Peptide Antibodies on the Proliferation, Activation, and Secretion of Cytokines of Fibroblast-Like Synoviocytes in Rheumatoid Arthritis In Vitro Co-Culture System
Mst1 promote mitochondrial dysfunction and apoptosis in oxidative stress-induced rheumatoid arthritis synoviocytes
In this study, we investigated the role of macrophage stimulating 1 (mst1) SIRT1 and AMPK-signaling pathway in oxidative stress-induced mitochondrial dysfunction and apoptosis seen in rheumatoid arthritis associated fibroblast-like synoviocytes (RA-FLSs). Mst1 mRNA and protein expression was significantly higher in hydrogen peroxide (H2O2) -treated RA-FLSs of untreated control. H2O2 treatment induced mitochondrial apoptotic pathway by activating caspase3 / 9 and Bax in RA-FLSs.
In addition, H2O2 treatment significantly reduced the mitochondrial membrane potential and mitochondrial state 3 and state 4 respiration, but increased reactive oxygen species (ROS). Mst1 silencing significantly reduce oxidative stress induced mitochondrial dysfunction and apoptosis in RA-FLSs. SIRT1 expression was reduced significantly in H2O2-treated RA-FLSs, but higher in H2O2-treated mst1-RA-FLSs silenced.
Pretreatment with selisistat (SIRT1-specific inhibitor) or compound C (AMPK antagonist) significantly reduces the survival and function of mitochondria in H2O2-treated mst1-RA-FLSs silenced by inhibiting the function or expression of SIRT1 SIRT1, respectively.
Description: A sandwich quantitative ELISA assay kit for detection of Human Fibroblast Growth Factor Receptor Like Protein 1 (FGFRL1) in samples from tissue homogenates or other biological fluids.
Human Fibroblast Growth Factor Receptor Like Protein 1 (FGFRL1) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Fibroblast Growth Factor Receptor Like Protein 1 (FGFRL1) in samples from tissue homogenates or other biological fluids.
Human Fibroblast Growth Factor Receptor Like Protein 1 (FGFRL1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fibroblast Growth Factor Receptor Like Protein 1 (FGFRL1) in Tissue homogenates and other biological fluids.
Human Fibroblast Growth Factor Receptor Like Protein 1 (FGFRL1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fibroblast Growth Factor Receptor Like Protein 1 (FGFRL1) in Tissue homogenates and other biological fluids.
Human Fibroblast Growth Factor Receptor Like Protein 1 (FGFRL1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fibroblast Growth Factor Receptor Like Protein 1 (FGFRL1) in Tissue homogenates and other biological fluids.
Human Fibroblast Growth Factor Receptor Like Protein 1 (FGFRL1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fibroblast Growth Factor Receptor Like Protein 1 (FGFRL1) in Tissue homogenates and other biological fluids.
Human Fibroblast Growth Factor Receptor Like Protein 1 (FGFRL1) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Fibroblast Growth Factor Receptor Like Protein 1 (FGFRL1) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Human Fibroblast Growth Factor Receptor Like Protein 1 (FGFRL1) ELISA Kit
Description: A Rabbit polyclonal antibody against Rat Fibroblast Growth Factor Receptor Like Protein 1 (FGFRL1). This antibody is labeled with APC-Cy7.
Rat Bladder PrimaCell2: Normal Bladder Fibroblasts
Description: A sandwich ELISA kit for detection of Fibroblast Growth Factor Receptor Like Protein 1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Rat Lung PrimaCell1: Normal Lung Fibroblasts Growth Medium
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast surface protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast surface protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast surface protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Fibroblast Growth Factor Receptor Like Protein 1 (FGFRL1) Polyclonal Antibody (Human)
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast growth factor 23 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast growth factor 23 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast growth factor 23 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast Growth Factor 19 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast Growth Factor 19 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast Growth Factor 19 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast growth factor 18 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast growth factor 18 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast growth factor 18 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Fibroblast Growth Factor 7 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Fibroblast Growth Factor 7 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Fibroblast Growth Factor 7 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Fibroblast Growth Factor 9 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Fibroblast Growth Factor 9 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Fibroblast Growth Factor 9 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Fibroblast growth factor 10 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Fibroblast growth factor 10 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Fibroblast growth factor 10 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast Growth Factor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast Growth Factor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast Growth Factor 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast growth factor 5 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast growth factor 5 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast growth factor 5 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast growth factor 15 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast growth factor 15 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast growth factor 15 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Fibroblast growth factor 16 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Fibroblast growth factor 16 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Fibroblast growth factor 16 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Basic Fibroblast growth factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Basic Fibroblast growth factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Basic Fibroblast growth factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast Growth Factor β1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast Growth Factor β1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast Growth Factor β1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Fibroblast Growth Factor Receptor Like Protein 1 (FGFRL1) Polyclonal Antibody (Human), FITC
Description: A sandwich ELISA for quantitative measurement of Rat Acidic Fibroblast Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Acidic Fibroblast Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Acidic Fibroblast Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Lung PrimaCell3: Normal Pulmonary Artery Fibroblasts Growth Medium
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Fibroblast growth factor 23 in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Fibroblast Growth Factor 6 (FGF6) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Fibroblast Growth Factor 6 (FGF6) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Fibroblast Growth Factor 6 (FGF6) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Fibroblast Growth Factor 6 (FGF6) in serum, plasma and other biological fluids.
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Rat Fibroblast Growth Factor 6 (FGF6)Serum, plasma and other biological fluids
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Fibroblast Growth Factor 9 (FGF9) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Fibroblast Growth Factor 9 (FGF9) in serum, plasma and other biological fluids.
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Rat Fibroblast Growth Factor 9 (FGF9)Serum, plasma and other biological fluids
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Fibroblast Growth Factor 3 (FGF3) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Fibroblast Growth Factor 3 (FGF3) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Fibroblast Growth Factor 3 (FGF3) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Fibroblast Growth Factor 3 (FGF3) in serum, plasma and other biological fluids.
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Rat Fibroblast Growth Factor 3 (FGF3)Serum, plasma and other biological fluids
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Fibroblast Growth Factor 4 (FGF4) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Fibroblast Growth Factor 4 (FGF4) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Fibroblast Growth Factor 4 (FGF4) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Fibroblast Growth Factor 4 (FGF4) in serum, plasma and other biological fluids.
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Rat Fibroblast Growth Factor 4 (FGF4)Serum, plasma and other biological fluids
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Fibroblast Growth Factor 9 (FGF9) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Fibroblast Growth Factor 9 (FGF9) in serum, plasma and other biological fluids.
These findings suggest that oxidative stress associated upregulation and activation mst1 promote mitochondrial dysfunction and apoptosis in RA-FLSs by inhibiting AMPK-SIRT1 pathway signaling. This shows mst1 axis-AMPK-SIRT1 is a potential target for the treatment of RA.
Shelby
